Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica

   

Screening and evaluation system for drought resistance in high-oleic acid and common peanut at the germination stage

MA Qun1,**,WANG Zhi-Hao1,**,YAN Lei1,LI Yu-Jiao1,WANG Jia-Qi1,LI Zhao1,LIU Wei1,AI Xin1,MA Qian-Chi1,WANG Xiao-Guang1,ZHONG Chao1,REN Jing-Yao1,LIU Xi-Bo1,ZHAO Shu-Li1,ZHANG He1, ZHAO Xin-Hua1,JIANG Chun-Ji1,WANG Jing1,*,YU Hai-Qiu1,2,*   

  1. 1 College of Agronomy, Shenyang Agricultural University, Shenyang 110161, Liaoning, China; 2 Liaoning Agricultural Vocational and Technical College, Yingkou 115009, Liaoning, China
  • Received:2025-01-27 Revised:2025-07-09 Accepted:2025-07-09 Online:2025-07-16 Published:2025-07-16
  • Supported by:
    This study was supported by the Liaoning Province Science and Technology Plan Joint Plan (2024-MSLH-417), the China Agriculture Research System of MOF and MARA (CARS-13), and the Department of Education Project by Liaoning Province (JYTMS20231293).

PDF

0

Abstract:

Drought is one of the major abiotic stresses limiting peanut production in Northeast China, and cultivating drought-resistant varieties remains the most effective strategy to mitigate its impact. This study aimed to investigate the effects of drought stress on the germination of high-oleic and common peanut varieties, and to evaluate their drought resistance at the germination stage. Polyethylene glycol (PEG-6000) solutions at concentrations of 15.0%, 17.5%, and 20.0% were used to simulate water deficit conditions, while a treatment without PEG served as the control. The most suitable concentration for drought resistance screening was identified, and several drought-related indices were measured during germination. Principal component analysis (PCA), regression analysis, and cluster analysis were employed for a comprehensive evaluation of drought resistance at the germination stage. The results showed that: (1) Under different PEG-6000 treatments, germination traits were not significantly affected at 15.0%, while most traits were significantly inhibited at 20.0%. Therefore, 17.5% PEG-6000 was determined to be the optimal concentration for screening drought resistance in peanut varieties during germination. (2) PCA reduced eight individual drought resistance indicators into three independent composite indices. The drought resistance of 38 peanut varieties was ranked using the membership function method and a comprehensive evaluation D-value. (3) Cluster analysis grouped 19 common and 19 high-oleic acid peanut varieties into four categories based on drought resistance: extremely strong (4 varieties), strong (11 varieties), moderate (12 varieties), and weak (11 varieties), accounting for 10.5%, 28.9%, 31.6%, and 28.9% of the total, respectively. (4) Stepwise regression analysis identified an optimal regression model. Four indicators—germination rate, vigor index, fresh root weight, and dry root weight—were found to be effective for evaluating drought resistance in high-oleic peanut varieties at the germination stage.

Key words: high oleic acid peanut, drought stress, germination, comprehensive evaluation, germplasm screening

[1] 江龙. 基于GIS的区域旱灾系统时空特性分析与脆弱性评估. 合肥工业大学硕士学位论文, 安徽合肥, 2014.
Jiang L. Spatiotemporal Characteristics Analysis and Vulnerability Assessment of Regional Drought System based on GIS. MS Thesis of Hefei University of Technology, Hefei, Anhui, China, 2014 (in Chinese with English abstract).

[2] 刘永惠, 沈一, 沈悦, 梁满, 沙琴, 张旭尧, 陈志德. 花生干旱诱导型启动子AhMYB44-11-Pro的克隆与功能分析. 作物学报, 2024, 50: 2157–2166.

Liu Y H, Shen Y, Shen Y, Liang M, Sha Q, Zhang X Y, Chen Z D. Cloning and functional analysis of drought-inducible promoter AhMYB44-11-Pro in peanut (Arachis hypogaea L.). Acta Agron Sin, 2024, 50: 2157–2166 (in Chinese with English abstract).

[3] 高伟, 吕登宇,苗利娟, 石磊, 黄冰艳, 张毛宁, 房元瑾, 王娟, 齐飞艳, 董文召, 等. 高油酸花生品种脂肪及脂肪酸积累动态分析. 中国油料作物学报, 2023, 45: 629–636.
Gao W, Lyu D Y, Miao L J, Shi L, Huang B Y, Zhang M N, Fang Y J, Wang J, Qi F Y, Dong W Z, et al. Dynamic analysis of fat and fatty acid accumulation in peanut varieties with high oleic acid. Chin J Oil Crop Sci, 2023, 45: 629–636 (in Chinese with English abstract).

[4] 谢安, 孙永罡, 白人海. 中国东北近50年干旱发展及对全球气候变暖的响应. 地理学报, 2003, 58(增刊1): 75–82.

Xie A, Sun Y G, Bai R H. Arid climate trend over northeastern China and its response to global warming. Acta Geogr Sin, 2003, 58(S1): 75–82 (in Chinese with English abstract).

[5] 刘振宏, 李娇, 孙艳云, 林中冠, 马林, 王若男, 王一. 辽宁西部农作物生长季干旱风险及降水满足度研究. 沙漠与绿洲气象, 2020, 14(4): 124–130.

Liu Z H, Li J, Sun Y Y, Lin Z G, Ma L, Wang R N, Wang Y. Study on drought risk in crop growing season and precipitation satisfaction in western Liaoning. Desert Oasis Meteor, 2020, 14(4): 124–130 (in Chinese with English abstract).

[6] 谢华光. 辽宁省农作物生长季降水量的变化及其满足度与干旱风险度. 贵州农业科学, 2022, 50(1): 131–138.

Xie H G. Precipitation variation, precipitation satisfaction and drought risk degree in growth season of crops in Liaoning province. Guizhou Agric Sci, 2022, 50(1): 131–138 (in Chinese with English abstract).

[7] 刘芳, 张哲, 王积军. 我国高油酸花生种植及应用技术研究进展. 中国油料作物学报, 2020, 42: 956–959.

Liu F, Zhang Z, Wang J J. Progress on production and technology development of high-oleic acid peanut in China. Chin J Oil Crop Sci, 2020, 42: 956–959 (in Chinese with English abstract).

[8] Dong W, Lyu H J, Xia G M, Wang M C. Does diacylglycerol serve as a signaling molecule in plants? Plant Signal Behav, 2012, 7: 472–475.

[9] 李猛. 辽宁省干旱发生频率分析及旱灾成因研究. 吉林水利, 2024, (6): 63–68.

Li M. Analysis of drought frequency and study on causes of drought in Liaoning Province. Jilin Water Resour, 2024, (6): 63–68 (in Chinese with English abstract).

[10] Huang A H. Oleosins and oil bodies in seeds and other organs. Plant Physiol, 1996, 110: 1055–1061.

[11] 王才斌, 成波, 郑亚萍, 沙继锋, 李安东, 孙秀山. 温度对花生出苗、幼苗生长及开花的影响. 花生学报, 2003, 32(4): 7–11.

Wang C B, Cheng B, Zheng Y P, Sha J F, Li A D, Sun X S. Effects of temperature to seed emergence, seedling growth and anthesis of peanut. J Peanut Sci, 2003, 32(4): 7–11 (in Chinese with English abstract).

[12] Cao D, Ma Y, Cao Z, Hu S, Li Z, Li Y, Wang K, Wang X, Wang J, Zhao K, et al. Coordinated lipid mobilization during seed development and germination in peanut (Arachis hypogaea L.). J Agric Food Chem, 2024, 72: 3218–3230.

[13] 张智猛, 万书波, 戴良香, 吴正峰, 陈静, 苗华荣. 花生萌芽期水分胁迫品种适应性及抗旱性评价. 干旱地区农业研究, 2009, 27(5): 173–182.

Zhang Z M, Wan S B, Dai L X, Wu Z F, Chen J, Miao H R. A study on appraisal of suitability and drought-resistance of different peanut varieties at germination stage. Agric Res Arid Areas, 2009, 27(5): 173–182 (in Chinese with English abstract).

[14] 金欣欣, 宋亚辉, 苏俏, 杨永庆, 李玉荣, 王瑾. 冀花系列高油酸花生抗旱性鉴定与综合评价. 作物学报, 2025, 51: 797–811.

Jin X X, Song Y H, Su Q, Yang Y Q, Li Y R, Wang J. Identification and comprehensive evaluation of drought resistance in high oleic acid Jihua peanut varieties. Acta Agron Sin, 2025, 51: 797–811 (in Chinese with English abstract).

[15] Khajeh-Hosseini M, Powell A A, Bingham I J. The interaction between salinity stress and seed vigour during germination of soyabean seeds. Seed Sci Technol, 2003, 31: 715–725.

[16] 赵玉坤, 高根来, 王向东, 甄胜虎, 宁慧云, 李宝珠. PEG模拟干旱胁迫条件下玉米种子的萌发特性研究. 农学学报, 2014, 4(7): 1–4.

Zhao Y K, Gao G L, Wang X D, Zhen S H, Ning H Y, Li B Z. Effects of polyethylene glycol (PEG) simulated drought stress on seed germination characters in maize. J Agric, 2014, 4(7): 1–4 (in Chinese with English abstract).

[17] 李培英, 孙宗玖, 阿不来提. PEG模拟干旱胁迫下29份偃麦草种质种子萌发期抗旱性评价. 中国草地学报, 2010, 32(1): 32–39.

Li P Y, Sun Z J, A B. Evaluation of drought resistance of 29 accessions of elytrigria Repens at seed germination stage under PEG-6000 stress. Chin J Grassland, 2010, 32(1): 32–39 (in Chinese with English abstract).

[18] 张智猛, 万书波, 戴良香, 宋文武, 陈静, 苗华荣. 花生品种芽期抗旱性指标筛选与综合性评价. 中国农业科技导报, 2010, 12(1): 85–91.
Zhang Z M, Wan S B, Dai L X, Song W W, Chen J, Miao H R. Index screening and comprehensive evaluation for drought resistance of peanut varieties at germination stage. J Agric Sci Technol, 2010, 12(1): 85–91 (in Chinese with English abstract).

[19] 张高华, 于树涛, 王鹤, 王旭达. 高油酸花生发芽期低温胁迫转录组及差异表达基因分析. 遗传, 2019, 41: 1050–1059.

Zhang G H, Yu S T, Wang H, Wang X D. Transcriptome profiling of high oleic peanut under low temperature during germination. Hereditas, 2019, 41: 1050–1059 (in Chinese with English abstract).

[20] 孙东雷, 卞能飞, 王幸, 邢兴华, 沈一, 徐泽俊, 齐玉军, 王晓军. 高油酸花生萌发期耐冷性综合评价及种质筛选. 核农学报, 2021, 35: 1263–1272.

Sun D L, Bian N F, Wang X, Xing X H, Shen Y, Xu Z J, Qi Y J, Wang X J. Comprehensive evaluation of cold tolerance and germplasm screening of high oleic acid peanut at germination stage. J Nucl Agric Sci, 2021, 35: 1263–1272 (in Chinese with English abstract).

[21] 王欢. 玉米萌芽期耐冷性种质筛选及其全基因组关联分析. 东北农业大学硕士学位论文, 黑龙江哈尔滨, 2019.
Wang H. Germplasm Screening and Genome-wide Association Analysis for Chilling Tolerance during Germination and Budding Stage in Maize. MS Thesis of Northeast Agricultural University, Harbin, Heilongjiang, China, 2019 (in Chinese with English abstract).

[22] 于树涛, 于国庆, 孙泓希, 任亮, 尤淑丽, 王虹, 周文雨, 王传堂. 高油酸花生品种(系)抗旱性综合评价. 分子植物育种, 2021, 19: 2747–2757.

Yu S T, Yu G Q, Sun H X, Ren L, You S L, Wang H, Zhou W Y, Wang C T. Comprehensive evaluation of drought resistance of high oleic acid peanut varieties (lines). Mol Plant Breed, 2021, 19: 2747–2757 (in Chinese with English abstract).

[23] 徐明慧, 于晓东, 马兴林, 关义新. 水分胁迫对玉米萌芽期贮藏物质利用效率的影响. 作物杂志, 2004, (6): 11–13.

Xu M H, Yu X D, Ma X L, Guan Y X. Effect of water stress on utilization efficiency of storage materials in maize germination stage. Crops, 2004, (6): 11–13 (in Chinese).

[24] 王玮, 邹琦, 杨军, 周燮. 水分胁迫条件下抗旱性不同小麦品种芽鞘生长的动态分析. 植物生理学通讯, 1999, 35: 359–362.

Wang W, Zou Q, Yang J, Zhou X. Dynamic analysis of bud sheath growth of wheat varieties with different drought resistance under water stress. Plant Physiol Commun, 1999, 35: 359–362 (in Chinese).

[25] 周广生. 水稻抗旱性早期鉴定指标筛选与节水机理的研究. 华中农业大学博士学位论文, 湖北武汉, 2006.
Zhou G S. Study on the Appraising Indices of Drought Resistance at the Early Growth Stages and Water Saving Mechanism of Rice. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2006 (in Chinese with English abstract).

[26] Nautiyal P C, Ravindra V. Drying and storage method to prolong seed viability and seedling vigour of Rabi-summer-produced groundnut. J Agron Crop Sci, 1996, 177: 123–128.

[27] 刘永惠, 詹成芳, 沈一, 陈志德. 不同花生品种(系)萌发期抗旱性鉴定评价. 植物遗传资源学报, 2016, 17: 233–238.

Liu Y H, Zhan C F, Shen Y, Chen Z D. Identification of drought tolerance in peanut varieties/lines at the germination stage. J Plant Genet Resour, 2016, 17: 233–238 (in Chinese with English abstract).

[28] 李俊明. 玉米耐冷性的数值分类研究. 生物数学学报, 1990, 5(1): 58–61.

Li J M. A numerical taxonomic study on maize cold tolerance. J Biomath, 1990, 5(1): 58–61 (in Chinese with English abstract).

[29] 彭玉琳, 崔晓漫, 张沥曼玲, 次旦卓嘎, 昌西. 不同生育期大麦抗旱性鉴定及抗旱指标筛选. 西北农林科技大学学报(自然科学版), 2024, 52(8): 36–48.
Peng Y L, Cui X M, Zhang L M L, Ci D Z G, Chang X. Identification of drought resistance and selection of drought resistance indicators in barley at different growth periods. J Northwest A&F Univ (Nat Sci Edn), 2024, 52(8): 36–48 (in Chinese with English abstract).

[30] Mateva K I, Chai H H, Mayes S, Massawe F. Root foraging capacity in Bambara groundnut (Vigna subterranea (L.) verdc.) core parental lines depends on the root system architecture during the pre-flowering stage. Plants (Basel), 2020, 9: 645.

[31] 杨秀丽, 宁东贤, 周红琴, 赵玉坤, 杨丽萍, 李楠. 干旱胁迫下EMS诱变花生后代突变体萌发期抗旱性评价. 山西农业科学, 2021, 49: 817–821.

Yang X L, Ning D X, Zhou H Q, Zhao Y K, Yang L P, Li N. Evaluation of drought resistance in the germination period of mutants induced by chemical mutagenesis under drought stress. J Shanxi Agric Sci, 2021, 49: 817–821 (in Chinese with English abstract).

[32] 鲁成凯, 卢家毅, 宋晓峰, 董晓娜, 于田利, 付春, 任建军, 于复欣, 乔利仙. 高油酸花生品种(系)耐碱性评价. 山东农业科学, 2023, 55(9): 25–31.

Lu C K, Lu J Y, Song X F, Dong X N, Yu T L, Fu C, Ren J J, Yu F X, Qiao L X. Evaluation on alkali tolerance of peanut varieties(lines)with high oleic acid. Shandong Agric Sci, 2023, 55(9): 25–31 (in Chinese with English abstract).

[33] 岳丽, 山其米克, 王卉, 再吐尼古丽·库尔班, 毛红艳, 刘敏. 新疆高粱种质资源萌发期抗旱性综合评价. 草地学报, 2024, 32: 553–561.
Yue L, Shan Q M K, Wang H, Zaituniguli·K E B, Mao H Y, Liu M. Comprehensive evaluation of drought resistance of sorghum germplasm resources during germination in Xinjiang. Acta Agrest Sin, 2024, 32: 553–561 (in Chinese with English abstract).

[34] Kim J E, Yu J, Ryu J H, Lee J H, Kim T W. Assessment of regional drought vulnerability and risk using principal component analysis and a Gaussian mixture model. Nat Hazards, 2021, 109: 707–724.

[35] 王明辉, 胡海珍, 殷辉, 熊飞. 三个花生品种主要农艺性状比较及相关和回归分析. 湖北农业科学, 2012, 51: 3950–3952.

Wang M H, Hu H Z, Yin H, Xiong F. Comparison and analysis on main agronomic characters of three peanut varieties. Hubei Agric Sci, 2012, 51: 3950–3952 (in Chinese with English abstract).

[36] 芦振华, 李绍伟, 殷君华, 邓丽, 苗建利, 李阳, 郭敏杰, 胡俊平, 任丽. 高油酸花生开农1768在区域试验中主要性状的变异分析. 中国种业, 2024, (1): 68–71.

Lu Z H, Li S W, Yin J H, Deng L, Miao J L, Li Y, Guo M J, Hu J P, Ren L. Variation analysis of main traits of high oleic acid peanut Kainong 1768 in regional trial. China Seed Ind, 2024, (1): 68–71 (in Chinese).

[1] WANG Mu, ZHUO Ga, ZHA Sang, XIRUO Qu-Zong, DAWA Dondup, GUO Gang-Gang, ZHANG Jing, ZHUO Ga, LHUNDRUP Namgyal. Genetic diversity analysis and comprehensive evaluation of Qingke germplasm based on six phenotypic traits [J]. Acta Agronomica Sinica, 2025, 51(6): 1526-1537.
[2] SONG Song-Quan, TANG Cui-Fang, CHENG Hong-Yan, WANG Cheng-Liang, YUAN Liang-Bing, ZUO Sheng. Endosperm development of cereal crops and its role in seed dormancy and germination [J]. Acta Agronomica Sinica, 2025, 51(5): 1133-1155.
[3] LU Wen-Jia, WANG Jun-Cheng, YAO Li-Rong, ZHANG Hong, SI Er-Jing, YANG Ke, MENG Ya-Xiong, LI Bao-Chun, MA Xiao-Le, WANG Hua-Jun. Genome-wide identification of PRX gene family and analysis of their expressions under drought stress in barley [J]. Acta Agronomica Sinica, 2025, 51(5): 1198-1214.
[4] WANG Lin, CHEN Xiao-Yu, ZHANG Wen-Meng-Long, WANG Si-Qi, CHENG Bing-Yun, CHENG Jing-Qiu, PAN Rui, ZHANG Wen-Ying. Molecular characteristics and functional analysis of HvMYB2 in response to drought stress in barley [J]. Acta Agronomica Sinica, 2025, 51(4): 873-887.
[5] JIN Xin-Xin, SONG Ya-Hui, SU Qiao, YANG Yong-Qing, LI Yu-Rong, WANG Jin. Identification and comprehensive evaluation of drought resistance in high oleic acid Jihua peanut varieties [J]. Acta Agronomica Sinica, 2025, 51(3): 797-811.
[6] JIANG You, MA Xue-Rong, ZHANG Bo, LI Chen-Jian. Evaluation of salt tolerance and screening of salt-tolerant germplasm of Sorghum sudanese during seed germination period [J]. Acta Agronomica Sinica, 2025, 51(3): 835-844.
[7] HOU Tian-Yu, DU Xiao-Jing, ZHAO Zhi-Qiang, REYIM Anwar, YIDAYETULA Abula, BUHALIQIEMU Abulizi, YUAN Jie, ZHANG Yan-Hong, WANG Feng-Bin. Evaluation of cold tolerance of japonica rice varieties at germination stage and construction of identification system [J]. Acta Agronomica Sinica, 2025, 51(3): 812-822.
[8] HUO Ru-Xue, GE Xiang-Han, SHI Jia, LI Xue-Rui, DAI Sheng-Jie, LIU Zhen-Ning, LI Zong-Yun. Functional analysis of the sweetpotato histidine kinase protein IbHK5 in response to drought and salt stresses [J]. Acta Agronomica Sinica, 2025, 51(3): 650-666.
[9] NIE Bo-Tao, LIU De-Quan, CHEN Jian, CUI Zheng-Guo, HOU Yun-Long, CHEN Liang, QIU Hong-Mei, WANG Yue-Qiang. Analysis and comprehensive evaluation of agronomic and quality traits of spring soybean varieties in northern China [J]. Acta Agronomica Sinica, 2024, 50(9): 2248-2266.
[10] YAN Feng, DONG Yang, LI Qing-Quan, ZHAO Fu-Yang, HOU Xiao-Min, LIU Yang, LI Qing-Chao, ZHAO Lei, FAN Guo-Quan, LIU Kai. Comprehensively evaluation on cold tolerance of foxtail millet varieties at germination stage [J]. Acta Agronomica Sinica, 2024, 50(9): 2207-2218.
[11] LIU Yong-Hui, SHEN Yi, SHEN Yue, LIANG Man, SHA Qin, ZHANG Xu-Yao, CHEN Zhi-De. Cloning and functional analysis of drought-inducible promoter AhMYB44-11- Pro in peanut (Arachis hypogaea L.) [J]. Acta Agronomica Sinica, 2024, 50(9): 2157-2166.
[12] LIU Xin-Yue, GUO Xiao-Yang, WANG Xin-Ru, XIN Da-Wei, GUAN Rong-Xia, QIU Li-Juan. Establishment of screening method for salt tolerance at germination stage and identification of salt-tolerant germplasms in soybean [J]. Acta Agronomica Sinica, 2024, 50(8): 2122-2130.
[13] LI Wen-Juan, WANG Li-Min, QI Yan-Ni, ZHAO Wei, XIE Ya-Ping, DANG Zhao, ZHAO Li-Rong, LI Wen, XU Chen-Meng, WANG Yan, ZHANG Jian-Ping. Functional analysis of flax LuWRI1a in response to drought and salt stresses [J]. Acta Agronomica Sinica, 2024, 50(7): 1750-1761.
[14] LI Xiao-Fei, GAO Hua-Wei, GUANG Hui, SHI Yu-Xin, GU Yong-Zhe, QI Zhao-Ming, QIU Li-Juan. Identification and evaluation of atrazine tolerance of soybean germplasm resources at germination stage and screening of excellent germplasm [J]. Acta Agronomica Sinica, 2024, 50(7): 1699-1709.
[15] QIAO Zhi-Xin, ZHANG Jie-Dao, WANG Yu, GUO Qi-Fang, LIU Yan-Jing, CHEN Rui, HU Wen-Hao, SUN Ai-Qing. Difference in germination characteristics of different winter wheat cultivars under drought stress [J]. Acta Agronomica Sinica, 2024, 50(6): 1568-1583.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd