不同耐荫性谷子对遮阴的生理响应及转录组分析

doi:10.3724/SP.J.1006.2025.54045

Abstract

Abstract:

To investigate the response mechanisms of foxtail millet cultivars with different shade tolerance to shading stress, Yugu 29 (YG29) and Baogu 25 (BG25) were used as experimental materials. Shading nets were applied during the grain filling stage to simulate shading conditions. We examined the differences between the two cultivars in yield traits, photosynthetic pigments, photosynthetic parameters, antioxidant enzyme activities, and transcriptomic profiles. The results showed that shading stress significantly reduced yield, photosynthetic rate, transpiration rate, and stomatal conductance, while significantly increasing chlorophyll a and b contents as well as the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT). Compared with BG25, YG29 exhibited higher yield, chlorophyll content, photosynthetic rate, and antioxidant enzyme activities, along with a lower chlorophyll a/b ratio, indicating greater tolerance to shading stress. Transcriptome analysis identified 2,961 and 2,966 differentially expressed genes (DEGs) in BG25 and YG29 under shading conditions, respectively. These DEGs were significantly enriched in 24 and 16 Gene Ontology (GO) categories and 13 and 6 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in BG25 and YG29, respectively. Four pathways were commonly enriched in both cultivars: photosynthesis-antenna proteins, porphyrin and chlorophyll metabolism, phenylpropanoid biosynthesis, and cyanoamino acid metabolism. Further analysis of key metabolic pathways revealed that, under shading stress, YG29 showed lower expression levels of chlorophyll degradation-related genes (NOL, HCAR, SGR, PPH, and RCCR), and higher expression levels of genes related to carbon fixation (RBC, RCA, PEPC, MDH, GAPC, and FBA) and antioxidant enzymes (PRX, CAT) compared with BG25. These genes may play critical roles in the enhanced shade tolerance of YG29.

Key words: foxtail millet, shading stress, physiological characteristics, yield, transcriptome

ZHU Can-can, LI Jun-xia, JING Ya, FU Sen-jie, QIN Na, WANG Chun-yi, DAI Shu-tao, WEI Xin, ZHANG Cheng-Yang. Physiological response and transcriptome analysis of foxtail millet with different shading tolerances under shading stress[J].Acta Agronomica Sinica, 0, (): 0-.

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Physiological response and transcriptome analysis of foxtail millet with different shading tolerances under shading stress

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