Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2025, Vol. 51 ›› Issue (8): 2152-2163.doi: 10.3724/SP.J.1006.2025.53017

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of nitrogen fertilizer reduction measures on yield and nitrogen use efficiency of spring maize in Jianghuai region

YOU Gen-Ji1,XIE Hao1,LIANG Yu-Wen1,LI Long2,WANG Yu-Ru1,JIANG Chen-Yang1,GUO Jian1,LI Guang-Hao1,*,LU Da-Lei1   

  1. 1 Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops / College of Agriculture, Yangzhou University, Yangzhou 225009, Jiangsu, China; 2 Suqian Zhongjiang Seed Industry Co., Ltd., Suqian 223800, Jiangsu, China
  • Received:2025-03-03 Revised:2025-04-25 Accepted:2025-04-25 Online:2025-08-12 Published:2025-05-06
  • Supported by:
    This study was supported by the Jiangsu Agriculture Science and Technology Innovation Fund (CX [23] 3117), the National Natural Science Foundation of China (32101828), the Jiangsu Agricultural Industry Technology System of China (JATS [2023] 454), the Priority Academic Programme Development of Jiangsu Higher Education Institutions, and the Blue Project of Yangzhou University.

PDF

21

Abstract:

Diversified substitution strategies for nitrogen (N) fertilizer reduction and efficiency enhancement are key measures for implementing fertilizer reduction initiatives, promoting green and high-quality development of maize production, and ensuring food security. This study investigated the effects of different N fertilizer reduction measures on the yield, N uptake, and utilization of spring maize (Zea mays L.) in Jianghuai region, using the widely cultivated cultivar Jiangyu 877 in the Huanghuaihai region of China. Six N application treatments were established: no N fertilizer (CK), conventional N application (N225), reduced N application (N180), and reduced N application combined with either a nitrification inhibitor (N180+D), attapulgite (N180+T), or biological bone powder (N180+B). The effects of these treatments on yield and its components, leaf area index (LAI), SPAD value, aboveground dry matter and N accumulation, N translocation, and nitrogen use efficiency (NUE) were analyzed. Results showed that ear length, ear diameter, grains per ear, 1000-grain weight, and overall yield were significantly higher under N225 compared to N180. However, the yields under N180+D, N180+T, and N180+B were improved relative to N180 and showed no significant difference compared to N225. These treatments also increased LAI at silking and milk stages, and enhanced SPAD values of ear leaves at the milk stage. Compared with N180, aboveground dry matter and N accumulation under N180+D, N180+T, and N180+B increased by 20.3%, 18.1%, and 21.7%, and by 14.5%, 5.1%, and 21.5%, respectively. Moreover, these treatments promoted dry matter and N translocation and improved the harvest index. Compared with N225, the average NUE of N180+D, N180+T, and N180+B increased by 17.3%, 10.0%, and 22.9% in 2023 and 2024. In conclusion, a 20% reduction in N combined with the application of nitrification inhibitors, attapulgite, or biological bone powder can stabilize yield while improving nitrogen use efficiency, providing both theoretical insight and technical support for green, cost-effective, and efficient maize production.

Key words: spring maize, yield, nitrogen fertilizer reduction, matter accumulation, nitrogen uptake and utilization

[1] 赵小强, 方鹏, 彭云玲, 高巧红, 曾文静, 任斌. 基于两个相关群体的玉米6个穗部性状QTL定位. 农业生物技术学报, 2018, 26: 729–742.

Zhao X Q, Fang P, Peng Y L, Gao Q H, Zeng W J, Ren B. QTL mapping for six ear-related traits based on two maize (Zea mays) related populations. J Agric Biotechnol, 2018, 26: 729–742 (in Chinese with English abstract).

[2] 李少昆, 赵久然, 董树亭, 赵明, 李潮海, 崔彦宏, 刘永红, 高聚林, 薛吉全, 王立春, 等. 中国玉米栽培研究进展与展望. 中国农业科学, 2017, 50: 1941–1959.

Li S K, Zhao J R, Dong S T, Zhao M, Li C H, Cui Y H, Liu Y H, Gao J L, Xue J Q, Wang L C, et al. Advances and prospects of maize cultivation in China. Sci Agric Sin, 2017, 50: 1941–1959 (in Chinese with English abstract).

[3] Luo N, Meng Q F, Feng P Y, Qu Z R, Yu Y H, Liu D L, Müller C, Wang P. China can be self-sufficient in maize production by 2030 with optimal crop management. Nat Commun, 2023, 14: 2637.

[4] 刘弋菊, 孔箐锌, 苏胜宝. 玉米氮素代谢机制的研究进展. 玉米科学, 2009, 17(1): 135–138.

Liu Y J, Kong Q X, Su S B. Study progress on maize nitrogen metabolism. J Maize Sci, 2009, 17(1): 135–138 (in Chinese with English abstract).

[5] Charles J Godfray H, Beddington J R, Crute I R, Haddad L, Lawrence D, Muir J F, Pretty J, Robinson S, Thomas S M, Toulmin C. Food security: the challenge of feeding 9 billion people. Science, 2010, 327: 812–818.

[6] Chen X P, Cui Z L, Fan M S, Vitousek P, Zhao M, Ma W Q, Wang Z L, Zhang W J, Yan X Y, Yang J C, et al. Producing more grain with lower environmental costs. Nature, 2014, 514: 486–489.

[7] 高雪健, 李广浩, 陆卫平, 陆大雷. 控释尿素与普通尿素配施对糯玉米产量和氮素吸收利用的影响. 植物营养与肥料学报, 2022, 28: 1614–1625.

Gao X J, Li G H, Lu W P, Lu D L. Effects of mixing controlled-release and normal urea on yield, nitrogen absorption and utilization in waxy maize. J Plant Nutr Fert, 2022, 28: 1614–1625 (in Chinese with English abstract).

[8] Sun C, Chen L, Zhai L M, Liu H B, Wang K, Jiao C, Shen Z Y. National assessment of nitrogen fertilizers fate and related environmental impacts of multiple pathways in China. J Clean Prod, 2020, 277: 123519.

[9] 周伟, 吕腾飞, 杨志平, 孙红, 杨莨杰, 陈勇, 任万军. 氮肥种类及运筹技术调控土壤氮素损失的研究进展. 应用生态学报, 2016, 27: 3051–3058.

Zhou W, Lyu T F, Yang Z P, Sun H, Yang L J, Chen Y, Ren W J. Research advances on regulating soil nitrogen loss by the type of nitrogen fertilizer and its application strategy. Chin J Appl Ecol, 2016, 27: 3051–3058 (in Chinese with English abstract).

[10] 曹兵, 高玮, 李洪杰, 杜梦扬, 王学霞, 陈延华, 倪小会, 赵萌, 谷佳林, 董淑祺, 等. 控释掺混肥对小麦-玉米轮作体系产量、氮肥利用效率及氨挥发的影响. 植物营养与肥料学报, 2024, 30: 873–885.
Cao B, Gao W, Li H J, Du M Y, Wang X X, Chen Y H, Ni X H, Zhao M, Gu J L, Dong S Q, et al. Effects of blended application of coated and common urea on yield, nitrogen use efficiency and ammonia volatilization in winter wheat–summer maize rotation system. J Plant Nutr Fert, 2024, 30: 873–885 (in Chinese with English abstract).

[11] 刘亚龙, 王鹏飞, 于爱忠, 王玉珑, 尚永盼, 杨学慧, 尹波, 张冬玲, 王凤. 绿肥还田条件下减氮对河西绿洲灌区玉米产量及N2O排放的影响. 作物学报, 2025, 51: 771–784.

Liu Y L, Wang P F, Yu A Z, Wang Y L, Shang Y P, Yang X H, Yin B, Zhang D L, Wang F. Effects of nitrogen reduction on maize yield and N2O emission under green manure returning in Hexi oasis irrigation area. Acta Agron Sin, 2025, 51: 771–784 (in Chinese with English abstract).

[12] 邓江茹, 刘文娟, 马琨, 贾彪, 姚举上. 减氮和控释肥替代对宁夏引黄灌区玉米产量及土壤无机氮的影响. 农业环境科学学报, 2024, 43: 2069–2079.

Deng J R, Liu W J, Ma K, Jia B, Yao J S. Impact of nitrogen reduction and controlled-release fertilizers on maize yields and soil inorganic nitrogen in the Yellow River irrigation area of Ningxia. J Agro Environ Sci, 2024, 43: 2069–2079 (in Chinese with English abstract). 

[13] 张忠庆, 高强. 硝化抑制剂2-氯-6-三氯甲基吡啶在农业中应用研究进展及其影响因素. 中国土壤与肥料, 2022, (4): 249–258.

Zhang Z Q, Gao Q. Effects of nitrification inhibitor nitrapyrin application in agricultural ecosystems and influencing factors: a review. Soil Fert Sci China, 2022, (4): 249–258 (in Chinese with English abstract).

[14] 冯志珍, 颜宏, 卢雨欣, 徐倩, 白亚妮, 赵文娟. 凹凸棒石基复合材料土壤改良效果研究: 以荒漠绿洲农田土壤为例. 中国环境科学, 2023, 43: 5328–5338.

Feng Z Z, Yan H, Lu Y X, Xu Q, Bai Y N, Zhao W J. Effects of attapulgite-based composites on soil improvement: take desert-oasis farmland soil as an example. China Environ Sci, 2023, 43: 5328–5338 (in Chinese with English abstract).

[15] 陶玲, 张倩, 张雪彬, 周雅琦, 孙梦洁, 任珺. 凹凸棒石-污泥共热解生物炭对玉米苗期生长特性和重金属富集效应的影响. 农业环境科学学报, 2020, 39: 1512–1520.

Tao L, Zhang Q, Zhang X B, Zhou Y Q, Sun M J, Ren J. Influence of biochar prepared by co-pyrolysis with attapulgite and sludge on maize growth and heavy metal accumulation. J Agron Environ Sci, 2020, 39: 1512–1520 (in Chinese with English abstract).

[16] 纪艺凝, 栾润宇, 王农, 徐应明, 罗文文, 孙约兵. 牛骨粉对Cd污染土壤修复效应和土壤肥力的影响. 环境科学学报, 2019, 39: 1645–1654.

Ji Y N, Luan R Y, Wang N, Xu Y M, Luo W W, Sun Y B. Effect of bovine bone meal on immobilization remediation and fertility of Cd contaminated soil. Acta Sci Circumst, 2019, 39: 1645–1654 (in Chinese with English abstract).

[17] 程前, 李广浩, 陆卫平, 陆大雷. 增密减氮提高夏玉米产量和氮素利用效率. 植物营养与肥料学报, 2020, 26: 1035–1046.

Cheng Q, Li G H, Lu W P, Lu D L. Increasing planting density and decreasing nitrogen rate increase yield and nitrogen use efficiency of summer maize. J Plant Nutr Fert, 2020, 26: 1035–1046 (in Chinese with English abstract).

[18] 孙延亮, 赵俊威, 刘选帅, 李生仪, 马春晖, 王旭哲, 张前兵. 施氮对苜蓿初花期光合日变化、叶片形态及干物质产量的影响. 草业学报, 2022, 31(9): 63–75.

Sun Y L, Zhao J W, Liu X S, Li S Y, Ma C H, Wang X Z, Zhang Q B. Effect of nitrogen application on photosynthetic daily variation, leaf morphology and dry matter yield of alfalfa at the early flowering growth stage. Acta Pratac Sin, 2022, 31(9): 63–75 (in Chinese with English abstract).

[19] 张盼盼, 李川, 张美微, 赵霞, 牛军, 乔江方. 氮肥减施下添加硝化抑制剂对夏玉米氮素累积转运和产量的影响. 中国农业科技导报, 2023, 25(6): 181–189.

Zhang P P, Li C, Zhang M W, Zhao X, Niu J, Qiao J F. Effect of nitrification inhibitor application on nitrogen accumulation and transportation and grain yield of summer maize under reduced nitrogen. J Agric Sci Technol, 2023, 25(6): 181–189 (in Chinese with English abstract).

[20] 张如梦, 李冬佳, 梁雄英, 董汶卿, 唐智萍, 陈国庆, 陈骏, 喻海峰, 王孝忠, 刘蕊, 等. 多形态氮和硝化抑制剂协同供应对土壤氮素转化的影响. 中国土壤与肥料, 2024, (7): 69–78.

Zhang R M, Li D J, Liang X Y, Dong W Q, Tang Z P, Chen G Q, Chen J, Yu H F, Wang X Z, Liu R, et al. Effects of combined applicaion of polymorphic nitrogen and nitrification inhibitors on soil nitrogen transformation. Soil Fert Sci China, 2024, (7): 69–78 (in Chinese with English abstract).

[21] 王亚菲, 石岩. 凹凸棒石在农业生产上应用进展. 耕作与栽培, 2016, 36(2): 69–72.

Wang Y F, Shi Y. The application progress of attapulgite in agricultural production. Tillage Cultiv, 2016, 36(2): 69–72 (in Chinese with English abstract).

[22] 赵文瑞, 孔群芳, 张文娟, 胡程凯, 林雨欣, 陶炳娇, 王国鑫, 彭可睿, 王聪, 赵宽. 骨粉生物质炭对酸性土壤的改良作用. 土壤学报, 2024, 61: 1299–1309.

Zhao W R, Kong Q F, Zhang W J, Hu C K, Lin Y X, Tao B J, Wang G X, Peng K R, Wang C, Zhao K. Alleviating effects of bone meal biochars on acidic soil. Acta Pedol Sin, 2024, 61: 1299–1309 (in Chinese with English abstract).

[23] 刘婷娜, 苏永中, 安芳娇, 牛子儒. 长期不同施肥运筹对玉米产量、氮素转运和土壤氮积累的影响. 中国土壤与肥料, 2024, (4): 108–118.

Liu T N, Su Y Z, An F J, Niu Z R. Effects of long-term different fertilization on maize yield, nitrogen transport and soil nitrogen accumulation. Soil Fert Sci China, 2024, (4): 108–118 (in Chinese with English abstract).

[24] 吕巨智, 石达金, 周勋波, 唐国荣, 李发桥, 贺囡囡, 谢小东, 谭贤杰, 邹成林, 程伟东, 等. 缓控释肥对春玉米干物质积累、产量和经济效益的影响. 山东农业科学, 2023, 55(3): 132–136.

Lyu J Z, Shi D J, Zhou X B, Tang G R, Li F Q, He N N, Xie X D, Tan X J, Zou C L, Cheng W D, et al. Effects of slow and controlled release fertilizer on dry matter accumulation, yield and economic benefit of spring maize. Shandong Agric Sci, 2023, 55(3): 132–136 (in Chinese with English abstract).

[25] 严旖旎, 石晓旭, 刘海翠, 单海勇, 刘旭杰, 张晋, 刘建, 李赢, 杨美英. 一次性施肥方式在夏玉米上的应用效果. 江苏农业科学, 2022, 50(21): 109–114.

Yan Y N, Shi X X, Liu H C, Shan H Y, Liu X J, Zhang J, Liu J, Li Y, Yang M Y. Application effect of one-time fertilization method on summer maize. Jiangsu Agric Sci, 2022, 50(21): 109–114 (in Chinese with English abstract).

[26] Wei S S, Wang X Y, Li G H, Qin Y Y, Jiang D, Dong S T. Plant density and nitrogen supply affect the grain-filling parameters of maize kernels located in different ear positions. Front Plant Sci, 2019, 10: 180.

[27] 朱紫鑫, 张玉璐, 贾靖, 李文璐, 赵露迪, 孟繁港, 盖红梅, 徐学欣, 赵长星. 不同品种(系)彩色小麦干物质积累转运和产量形成分析. 华北农学报, 2023, 38(5): 128–138.

Zhu Z X, Zhang Y L, Jia J, Li W L, Zhao L D, Meng F G, Ge H M, Xu X X, Zhao C X. Analysis of dry matter accumulation and remobilization, and yield formation in different varieties (lines) of colored-grain wheat. Acta Agric Boreali-Sin, 2023, 38(5): 128–138 (in Chinese with English abstract).

[28] 王帅兵, 肖文贤, 夏鸾金玥, 李兰, 彭淑娴, 王克勤, 杨树东. 等高反坡阶对红壤坡耕地作物生长发育的影响. 中国水土保持科学(中英文), 2024, 22(6): 106–115.

Wang S B, Xiao W X, Xia L J Y, Li L, Peng S X, Wang K Q, Yang S D. Effects of contour reverse-slope terrace on crop growth and development in red soil sloping farmland. Sci Soil Water Conser, 2024, 22(6): 106–115 (in Chinese with English abstract).

[29] 郑贝贝, 赵威, 刘松涛, 张亚菲. 不同新型尿素与普通尿素减氮配施对夏玉米氮利用、产量及土壤氮平衡的影响. 江苏农业科学, 2024, 52(2): 90–97.

Zheng B B, Zhao W, Liu S T, Zhang Y F. Effects of reduced nitrogen application of different novel urea and common urea on nitrogen utilization, yield and soil nitrogen balance of summer maize. Jiangsu Agric Sci, 2024, 52(2): 90–97 (in Chinese with English abstract).

[30] 刘秉鑫, 孟浩峰, 李玲玲, 谢军红, 周永杰, 田鑫, 王春艳, 张国超. 有机肥替代基施化肥氮对旱作玉米叶片光合性能、产量及氮肥利用效率的影响. 玉米科学, 2024, 32(9): 96–107.

Liu B X, Meng H F, Li L L, Xie H J, Zhou Y J, Tian X, Wang C Y, Zhang G C. Effects of organic fertilizer substitution for basal chemical nitrogen on photosynthetic performance, yield, and nitrogen use efficiency in rainfed maize. J Maize Sci, 2024, 32(9): 96–107 (in Chinese with English abstract).

[31] Ruiz A, Archontoulis S V, Borrás L. Kernel weight relevance in maize grain yield response to nitrogen fertilization. Field Crops Res, 2022, 286: 108631.

[32] Zhai L C, Xu P, Zhang Z B, Wei B H, Jia X L, Zhang L H. Improvements in grain yield and nitrogen use efficiency of summer maize by optimizing tillage practice and nitrogen application rate. Agron J, 2019, 111: 666–676.

[1] YANG Ting-Ting, CHEN Juan, ABDUL Rehman, LI Jing, YAN Su-Hui, WANG Jian-Lai, LI Wen-Yang. Effects of weak light post-anthesis on dry matter accumulation and translocation, grain yield, and starch quality in soft wheat [J]. Acta Agronomica Sinica, 2025, 51(8): 2204-2219.
[2] LI Yi-Qian, XU Shou-Zhen, LIU Ping, MA Qi, XIE Bin, CHEN Hong. Genome-wide association study of yield components using a 40K SNP array and identification of a stable locus for boll weight in upland cotton (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2025, 51(8): 2128-2138.
[3] FAN You-Zhong, WANG Xian-Ling, WANG Zong-Kai, WANG Chun-Yun, WANG Tian-Yao, XIE Jie, KUAI Jie, WANG Bo, WANG Jing, XU Zheng-Hua, ZHAO Jie, ZHOU Guang-Sheng. Effects of straw incorporation combined with nitrogen management on photosynthetic efficiency and yield of rapeseed following rice [J]. Acta Agronomica Sinica, 2025, 51(8): 2139-2151.
[4] WU Bin, CAO Yong-Gang, HU Fa-Long, YIN Wen, FAN Zhi-Long, FAN Hong, CHAI Qiang. Compensation effect of no-tillage rotation on yield reduction of nitrogen- reduced wheat [J]. Acta Agronomica Sinica, 2025, 51(7): 1959-1968.
[5] WU Liu-Ge, CHEN Jian, ZHANG Xin, DENG Ai-Xing, SONG Zhen-Wei, ZHENG Cheng-Yan, ZHANG Wei-Jian. Changes in yield and quality traits of nationally approved winter wheat varieties in China over last twenty years [J]. Acta Agronomica Sinica, 2025, 51(7): 1814-1826.
[6] LI Qiu-Yun, LI Shi-Gui, FAN Jun-Liang, LIU Hao-Tian, ZHAO Xiao-Bin, LYU Shuo, WANG Yan-Hao, YUE Yun, ZHANG Ning, SI Huai-Jun. Effects of ionic zinc and nano-zinc on physiological characteristics, yield, and quality of potato [J]. Acta Agronomica Sinica, 2025, 51(7): 1838-1849.
[7] ZHAO Jia-Wen, LI Zi-Hong, OU Xing-Yu, WANG Yi-Lang, DING Xiao-Fei, LIANG Yue-Yao, DING Wen-Jin, ZHANG Hai-Peng, MA Shang-Yu, FAN Yong-Hui, HUANG Zheng-Lai, ZHANG Wen-Jing. Effects of nitrogen and potassium fertilizer management on grain yield and quality of weak-gluten wheat [J]. Acta Agronomica Sinica, 2025, 51(7): 1914-1933.
[8] WAN Shu-Bo, ZHANG Jia-Lei, GAO Hua-Xin, WANG Cai-Bin. Advances and prospects of high-yield peanut cultivation in China [J]. Acta Agronomica Sinica, 2025, 51(7): 1703-1711.
[9] LI Bing-Lin, YE Xiao-Lei, XIAO Hong, XIAO Guo-Bin, LYU Wei-Sheng, LIU Jun-Quan, REN Tao, LU Zhi-Feng, LU Jian-Wei. Effects of magnesium fertilization rates on rapeseed yield, magnesium uptake, and yield loss caused by frost damage [J]. Acta Agronomica Sinica, 2025, 51(7): 1850-1860.
[10] HUO Jian-Zhe, YU Ai-Zhong, WANG Yu-Long, WANG Peng-Fei, YIN Bo, LIU Ya-Long, ZHANG Dong-Ling, JIANG Ke-Qiang, PANG Xiao-Neng, WANG Feng. Effect of organic manure substitution for chemical fertilizer on yield, quality, and nitrogen utilization of sweet maize in oasis irrigation areas [J]. Acta Agronomica Sinica, 2025, 51(7): 1887-1900.
[11] DONG Wei-Jin, ZHANG Ya-Feng, LI Qi-Yun, LU Yang, ZHANG Zheng-Kun, SUI Li. Effects of Beauveria bassiana colonization on maize growth and yield under elevated CO2 concentration [J]. Acta Agronomica Sinica, 2025, 51(7): 1874-1886.
[12] CHEN Ru-Xue, SUN Li-Fang, ZHANG Xin-Yuan, MU Hai-Meng, ZHANG Yong-Xin, YUAN Li-Xue, PENG Shi-Le, WANG Zhuang-Zhuang, WANG Yong-Hua. Effects of combined straw returning and microbial inoculant application on carbon-nitrogen metabolism in flag leaves and yield formation in winter wheat [J]. Acta Agronomica Sinica, 2025, 51(7): 1901-1913.
[13] LI Zi-Xiang, HUANG Rong, WANG Zhi-Chao, LI Hong-Yan, TAN Jun-Xing, CHENG Yu, DU Xue-Zhu, SHENG Feng. Effects of poly-γ-glutamate acid on lodging resistance of direct seeding rice [J]. Acta Agronomica Sinica, 2025, 51(6): 1654-1664.
[14] CUI Xin, GU He-He, SONG Yi, ZHANG Zhe, LIU Shi-Shi, LU Zhi-Feng, REN Tao, LU Jian-Wei. Effects of potassium fertilizer application rates on rapeseed yield and potassium absorption and yield reduction caused by frost damage [J]. Acta Agronomica Sinica, 2025, 51(6): 1629-1642.
[15] YAN Shang-Long, WANG Qi-Ming, CHAI Qiang, YIN Wen, FAN Zhi-Long, HU Fa-Long, LIU Zhi-Peng, WEI Jin-Gui. Grain yield and quality of maize in response to dense density and intercropped peas in oasis irrigated areas [J]. Acta Agronomica Sinica, 2025, 51(6): 1665-1675.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wang Lanzhen;Mi Guohua;Chen Fanjun;Zhang Fusuo. Response to Phosphorus Deficiency of Two Winter Wheat Cultivars with Different Yield Components[J]. Acta Agron Sin, 2003, 29(06): 867 -870 .
[2] YANG Jian-Chang;ZHANG Jian-Hua;WANG Zhi-Qin;ZH0U Qing-Sen. Changes in Contents of Polyamines in the Flag Leaf and Their Relationship with Drought-resistance of Rice Cultivars under Water Deficiency Stress[J]. Acta Agron Sin, 2004, 30(11): 1069 -1075 .
[3] Yan Mei;Yang Guangsheng;Fu Tingdong;Yan Hongyan. Studies on the Ecotypical Male Sterile-fertile Line of Brassica napus L.Ⅲ. Sensitivity to Temperature of 8-8112AB and Its Inheritance[J]. Acta Agron Sin, 2003, 29(03): 330 -335 .
[4] Wang Yongsheng;Wang Jing;Duan Jingya;Wang Jinfa;Liu Liangshi. Isolation and Genetic Research of a Dwarf Tiilering Mutant Rice[J]. Acta Agron Sin, 2002, 28(02): 235 -239 .
[5] WANG Li-Yan;ZHAO Ke-Fu. Some Physiological Response of Zea mays under Salt-stress[J]. Acta Agron Sin, 2005, 31(02): 264 -268 .
[6] TIAN Meng-Liang;HUNAG Yu-Bi;TAN Gong-Xie;LIU Yong-Jian;RONG Ting-Zhao. Sequence Polymorphism of waxy Genes in Landraces of Waxy Maize from Southwest China[J]. Acta Agron Sin, 2008, 34(05): 729 -736 .
[7] XING Guang-Nan, ZHOU Bin, ZHAO Tuan-Jie, YU De-Yue, XING Han, HEN Shou-Yi, GAI Jun-Yi. Mapping QTLs of Resistance to Megacota cribraria (Fabricius) in Soybean[J]. Acta Agronomica Sinica, 2008, 34(03): 361 -368 .
[8] Qi Zhixiang;Yang Youming;Zhang Cunhua;Xu Chunian;Zhai Zhixi. Cloning and Analysis of cDNA Related to the Genes of Secondary Wall Thickening of Cotton (Gossypium hirsutum L.) Fiber[J]. Acta Agron Sin, 2003, 29(06): 860 -866 .
[9] ZHENG Yong-Mei;DING Yan-Feng;WANG Qiang-Sheng;LI Gang-Hua;WANG Hui-Zhi;WANG Shao-Hua. Effect of Nitrogen Applied before Transplanting on Tillering and Nitrogen Utilization in Rice[J]. Acta Agron Sin, 2008, 34(03): 513 -519 .
[10] QIN En-Hua;YANG Lan-Fang;. Selenium Content in Seedling and Selenium Forms in Rhizospheric Soil of Nicotiana tabacum L.[J]. Acta Agron Sin, 2008, 34(03): 506 -512 .
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd