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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1967-1973.doi: 10.3724/SP.J.1006.2010.01967

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Changes of Cell Physiology and Texture of Tobacco Leaf during Bulk Flue- Curing

SONG Zhao-Peng1,2,GONG Chang-Rong2,WU Sheng-Jiang2,LIU Wen-Bo3,HE Fan2,LIU Chuan2,ZHANG Yong-Gang2,ZHANG Wei-Jiang1,4,*   

  1. 1 College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; 2 College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China; 3 China Tobacco Industrial Company of Zhejiang, Hangzhou 310000, China; 4 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-01-12 Revised:2010-06-28 Online:2010-11-12 Published:2010-08-30
  • Contact: ZHANG Wei-Jian,E-mail:zhangweij@caas.net.cn

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Abstract: During recent years, the bulk curing barn has rapidly developed and greatly promoted the production of flue-cured tobacco in China. However, some problems, such as the lightening colours, the smoothness and close structure of tobacco leaf, occurred along with the popularizing of flue-curing barn application. In order to meet the quality requirement of the raw material of Chinese-style cigarette, it has been urgent to solve those problems for the tobacco production with high-quality. The changes of the cell wall hydrolases and components play an important role in affecting the physiological changes of post-harvest plant. The differences in the texture of tobacco leaves would affect the organizational structure, morphostructure, internal components and usability. In order to optimize flue-curing technology and to improve the usability of tobacco leaves, we conducted the experiment to investigate the changes of cell physiology and texture of upper tobacco leaves of Zhongyan 100 along with wet and dry bulb temperatures during flue-curing using the electric-heated flue-curing barn designed and made by Henan Agricultural University. The results showed that the respiration rate of tobacco leaf peaked at 38℃ with a value of carbon dioxide content of about 1.80% during curing, and then the respiration rate progressively decreased since 48℃. The activities of pectinesterase and cellulase were higher during 38~54℃ than other temperature conditions. The pectin methylesterases (PME) and cellulase activities were the highest at 42℃, while the polygalacturonase activity (PG) was highest at 48℃. The PG and PME promoted the hydrolysis process of protopectin. Meanwhile, the protopectin, the total pectin and cellulose contents steadily decreased during curing. Texture testing parameters (except cohesiveness) of tobacco leaves changed significantly at the temperatures from 38℃ to 48℃. Among them hardness and chewiness had a maximum value at 42℃, tensile and shear force got their maximum values at 38℃. Cohesiveness, tensile and shear force reached the minimum value at 48℃ during flue-curing. The correlation between the soluble pectin and resilience was significantly negative while the correlations of protopectin, the total pectin, cellulose with resilience, tensile were significantly or highly significantly positive (P<0.05). These results indicated that the texture of tobacco leaves became mealy just due to the decrease of the cell binding force in a way during flue-curing. In other words, it could be resulted from the constant degradation of cell wall substances. This research indicated that dynamic change of shear force parameter was more intuitive. Resilience and tensile could be used as texture parameters to study the change of cell physiology of tobacco leaves. The main factors to make a significant difference in texture of tobacco leaves were the changes of cell physiology during flue-curing.

Key words: Flue-curing, Flue-cured tobacco, Cell physiology, Texture

[1]Wang W-F(王卫峰), Chen J-H(陈江华), Song Z-P(宋朝鹏), Gong C-R(宫长荣). The research progress on bulk curing barn. Chin Tob Sci (中国烟草科学), 2005, 26(3): 12–14 (in Chinese with English abstract)
[2]Xu X-H(徐秀红), Sun F-S(孙福山), Wang Y(王永), Zhao B(赵兵), Liu J-Y(刘建永), Deng Q-Q(邓启强), Ding Z-Y(丁志勇). Current situation and further direction on research and application of bulk curing barn in China. Chin Tob Sci (中国烟草科学), 2008, 29(4): 54–56 (in Chinese with English abstract)
[3]Hang Y(黄勇), Zhou J-H(周冀衡), Yang H-Q(杨虹琦), Liu J-L(刘建利), Wang Y(王勇), Zhou Y-H(周义和), Huang Y(黄琰), He X-L(何小力). Cytological observation of leaf and chloroplast during the flue-cured tobacco maturation. Acta Agron Sin (作物学报), 2006, 32(11): 1767–1770 (in Chinese with English abstract)
[4]Wang Y-L(王艳丽), Liu G-S(刘国顺). Effects of phosphorus fertilizer rates on cell wall matter content and thickness of tobacco leaf. Tob Sci Technol (烟草科技), 2005, (5): 41–44 (in Chinese with English abstract)
[5]Goulao L F, Santos J, Sousa I, Oliveira C M. Patterns of enzymatic activity of cell wall-modifying enzymes during growth and ripening of apples. Postharvest Biol Tec, 2007, 43: 307–318
[6]Deng Y, Wu Y, Li Y F. Changes in firmness, cell wall composition and cell wall hydrolases of grapes stored in high oxygen atmospheres. Food Res Int, 2005, 38: 769–776
[7]Wang R-X(王瑞新). Tobacco Chemistry (烟草化学). Beijing: China Agriculture Press, 2003. pp 26–44, 156–167 (in Chinese)
[8]Yan K-Y(闫克玉), Yan H-X(闫洪洋), Li X-B(李兴波), Mao W-L(毛文龙), Bo Y-C(薄云川). Comparative analysis of cell wall substances in flue-cured tobacco leaf. Tob Sci Technol (烟草科技), 2005, (10): 6–11 (in Chinese with English abstract)
[9]Yan K-Y(闫克玉), Liu F-Z(刘凤珠). Degrading enzyme in cell wall substances of tobacco. Biotechnol (生物技术), 2001, 11(4): 19–22 (in Chinese with English abstract)
[10]Li W-F(李卫芳), Zhang M-N(张明农), Lin P-Z (林培章), Li H-L(李汉玲). Varation of the respiratory rate and drying rate during the flue curing of tobacco. J Nanjing Norm Univ (Nat Sci) (南京师大学报·自然科学版), 2000, 23(4): 112–115 (in Chinese with English abstract)
[11]Zhang X-Y(张晓远), Bi Q-W(毕庆文), Wang J(汪健), Wang H-M(王海明), Li G(黎根), Liu G-S(刘国顺). Effects of temperature, humidity, and duration of yellowing stage on respiration rate and chemistry of upper leaves. Tob Sci Technol (烟草科技), 2009, (6): 56–59 (in Chinese with English abstract)
[12]Wang N-R(王能如), Li Z-H(李章海), Xu Z-H(徐增汉), Li W-F(李卫芳), Xu Q-K(徐庆凯). Changes of thickness and dissecting structure of upper leaves of flue-cured tobacco during curing. Tob Sci Technol (烟草科技), 2005, (9): 29–31 (in Chinese with English abstract)
[13]Pan X-J(潘秀娟), Tu K(屠康). Comparison of texture properties of post-harvested apples using texture profile analysis. Trans CSAE (农业工程学报), 2005, 21(3): 166–170 (in Chinese with English abstract)
[14]Jin C H, Suo B, Kan J, Wang H M, Wang Z J. Changes in cell wall polysaccharide of harvested peach fruit during storage. J Plant Physiol Mol Biol, 2006, 32: 657–664
[15]Lin F-D(林芳栋), Jiang Z-J(蒋珍菊), Liao S(廖珊), You J(游娟), Li C-X(李朝学). The summary of texture analyzer and its application in the evaluation for food quality. Life Sci Instr (生命科学仪器), 2009, 7(5): 61–63 (in Chinese with English abstract)
[16]Rastogi V K, Nguyen L T, Balasubramanian V M. Effect of pretreatments on carrot texture after thermal and pressure-assisted thermal processing. J Food Eng, 2008, 88: 541–547
[17]Segade S R, Rolle L, Gerbi V, Orriols I. Phenolic ripeness assessment of grape skin by texture analysis. J Food Compos Anal, 2008, 21: 644–649
[18]Bai B-Z(白宝璋), Kong X-S(孔祥生), Wang Y-K(王玉昆). Plant Physiology—Experiment Course II (植物生理学——实验教程下). Beijing: China Agricultural Technology Press, 1996. p 13 (in Chinese)
[19]Ning Z-X(宁正祥). Food Ingredient Analysis (食品成分分析手册). Beijing: China Light Industry Press, 1998. pp 45–49, 690–693 (in Chinese)
[20]Han Y-S(韩雅珊). A Laboratory Guidance of Food Chemistry (食品化学实验指导). Beijing: Beijing Agricultural University Press, 1996. pp 4–5, 32–41, 109–111 (in Chinese)
[21]Hagerman A E, Austin P J. Continuous spectrophotometric assay for plant pectinmethylesterase. J Agric Food chem, 1986, 34: 440–444
[22]Fischer R L, Bennett A B. Role of cell wall hydrolases in fruit ripening. Annu Rev Plant Physiol Plant Mol Biol, 1991, 42: 675–703
[23]Wei H-P(魏和平), Li R Q(利容千), Wang J B(王建波), Wu G L(吴甘霖), Wu Y(吴彦). Ultrastructural localization of cellulase activity in lytic root cortical cell during aerenchyma formation of flooded maize. Acta Agron Sin (作物学报), 2006, 32(1): 84–87 (in English with Chinese abstract)
[24]Gong C-R(宫长荣), Zhou Y-H(周义和), Yang H-W(杨焕文). Introduction for three stage curing of flue-cured tobacco (烤烟三段式烘烤导论). Beijing: Science Press, 2005. pp 92–94, 62–185 (in Chinese)
[25]Holcroft D M, Lin H T, Ketsa S. Harvesting and storage. In: Menzel C, Mitra S K, Waite G eds. Litchi and Longan: Botany, Cultivation and Uses. Wallingford, UK: CAB International, 2005. pp 273–295
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