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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (11): 1967-1973.doi: 10.3724/SP.J.1006.2010.01967

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Changes of Cell Physiology and Texture of Tobacco Leaf during Bulk Flue- Curing

SONG Zhao-Peng1,2,GONG Chang-Rong2,WU Sheng-Jiang2,LIU Wen-Bo3,HE Fan2,LIU Chuan2,ZHANG Yong-Gang2,ZHANG Wei-Jiang1,4,*   

  1. 1 College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; 2 College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China; 3 China Tobacco Industrial Company of Zhejiang, Hangzhou 310000, China; 4 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-01-12 Revised:2010-06-28 Online:2010-11-12 Published:2010-08-30
  • Contact: ZHANG Wei-Jian,E-mail:zhangweij@caas.net.cn

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Abstract: During recent years, the bulk curing barn has rapidly developed and greatly promoted the production of flue-cured tobacco in China. However, some problems, such as the lightening colours, the smoothness and close structure of tobacco leaf, occurred along with the popularizing of flue-curing barn application. In order to meet the quality requirement of the raw material of Chinese-style cigarette, it has been urgent to solve those problems for the tobacco production with high-quality. The changes of the cell wall hydrolases and components play an important role in affecting the physiological changes of post-harvest plant. The differences in the texture of tobacco leaves would affect the organizational structure, morphostructure, internal components and usability. In order to optimize flue-curing technology and to improve the usability of tobacco leaves, we conducted the experiment to investigate the changes of cell physiology and texture of upper tobacco leaves of Zhongyan 100 along with wet and dry bulb temperatures during flue-curing using the electric-heated flue-curing barn designed and made by Henan Agricultural University. The results showed that the respiration rate of tobacco leaf peaked at 38℃ with a value of carbon dioxide content of about 1.80% during curing, and then the respiration rate progressively decreased since 48℃. The activities of pectinesterase and cellulase were higher during 38~54℃ than other temperature conditions. The pectin methylesterases (PME) and cellulase activities were the highest at 42℃, while the polygalacturonase activity (PG) was highest at 48℃. The PG and PME promoted the hydrolysis process of protopectin. Meanwhile, the protopectin, the total pectin and cellulose contents steadily decreased during curing. Texture testing parameters (except cohesiveness) of tobacco leaves changed significantly at the temperatures from 38℃ to 48℃. Among them hardness and chewiness had a maximum value at 42℃, tensile and shear force got their maximum values at 38℃. Cohesiveness, tensile and shear force reached the minimum value at 48℃ during flue-curing. The correlation between the soluble pectin and resilience was significantly negative while the correlations of protopectin, the total pectin, cellulose with resilience, tensile were significantly or highly significantly positive (P<0.05). These results indicated that the texture of tobacco leaves became mealy just due to the decrease of the cell binding force in a way during flue-curing. In other words, it could be resulted from the constant degradation of cell wall substances. This research indicated that dynamic change of shear force parameter was more intuitive. Resilience and tensile could be used as texture parameters to study the change of cell physiology of tobacco leaves. The main factors to make a significant difference in texture of tobacco leaves were the changes of cell physiology during flue-curing.

Key words: Flue-curing, Flue-cured tobacco, Cell physiology, Texture

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