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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (07): 1179-1186.doi: 10.3724/SP.J.1006.2013.01179


Analysis of Resistance to Verticillium Wilt in Cotton by Reciprocal Grafting and Real-time Quantitative PCR

HAO Jun-Jie1,**,*,HU Yu-Wei1,**,GUO Xiao-Qin2,ZhAO Fu-An3,JIA Xin-He4,GUO Li-Juan1,ZHANG Zhi-Xing1,WANG Qing-Dong5,*   

  1. 1 Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Ministry of Agriculture / Henan Key Laboratory for Control of Crop Diseases and Insect Pests / Plant Protection Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2 College of Chemical and Food Engineering, Zhongzhou University, Zhengzhou 450044, China; 3 Industrial Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 4 Zhengzhou Institute of Agriculture and Forestry Sciences, Zhengzhou 450002, China; 5 Department of Biology Engineering, Zhengzhou University, Zhengzhou 450001, China
  • Received:2012-11-29 Revised:2013-03-11 Online:2013-07-12 Published:2013-04-23
  • Contact: 郝俊杰, E-mail: haojjds@yahoo.com.cn; 王庆东, E-mail: qdwang@zzu.edu.cn


In an attempt to better understand the resistance mechanism of controlling cotton Verticillium wilt by locating responsible for resistance in the plant, reciprocal grafts were made between resistant (Gossypium barbadense, Hai 7124 and Pima 90) and susceptible (G. hirsutum, Jimian 11) genotypes. The grafting combinations were inoculated with Verticillium dahliae. Quantitative monitoring of colonization of V. dahliae in different parts of the plant was analyzed using Real-time quantitative PCR (qPCR). When comparing resistant/susceptible or susceptible/resistant grafted combinations with their resistant and susceptible self-graft checks, respectively, disease index of reciprocal grafts and IC (infection coefficient) values of different parts of the plant were nearly within a range between their corresponding checks, respectively, indicating the pathogens could be transferred through the graft union in reciprocal grafts. The disease index was significantly related with average IC values in different parts of plant in different cotton types. Susceptible/resistant grafted combinations showed basal stem of resistant genotypes had an important role on inhibiting translocation of the pathogens, and resistant/susceptible grafted combinations also showed upper stem above graft union with the resistant ability to V. dahliae in resistant genotypes, which indicated that the resistant genotype G. barbadense has the whole-plant of resistance to V. dahliae. In addition, it seems that all parts of the plant in susceptible genotypes (G. hirsutum) were susceptible to V. dahliae.

Key words: Cotton Verticillium wilt, Reciprocal grafting, Real-time quantitative PCR

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