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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (07): 1187-1199.doi: 10.3724/SP.J.1006.2013.01187


Association Analysis of Yield Traits with Molecular Markers in Huang-Huai River Valley Winter Wheat Region, China

ZHANG Guo-Hua1,GAO Ming-Gang1,2,ZHANG Gui-Zhi1,SUN Jin-Jie1,JIN Xue-Mei1,WANG Chun-Yang1,ZHAO Yan1,LI Si-Shen1,*   

  1. 1 State Key Laboratory of Crop Biology / Agronomy College of Shandong Agricultural University, Tai’an 271018, China; 2 Department of Biological and Agricultural Engineering, Weifang University, Weifang 261061, China
  • Received:2013-01-10 Revised:2013-03-11 Online:2013-07-12 Published:2013-04-23
  • Contact: 李斯深, E-mail: ssli@sdau.edu.cn, Tel: 0538-8242903 E-mail:ssli@sdau.edu.cn


Grain yield is an important breeding target in wheat, which is controlled by complex genetic factors and strongly influenced by environments. In this study, the yield and yield-related traits of 128 wheat varieties/lines from the Huang-Huai River Valley Winter Wheat Region were evaluated in four growing environments. A total of 64 SSR, 27 EST-SSR and 47 functional markers were used to genotype these varieties/lines. Ninety-one SSR and EST-SSR markers produced 315 alleles, with 2–7 alleles on each locus and an average of 3.5. Forty-seven functional markers produced 107 alleles, with 2–5 alleles per locus and an average of 2.3. A total of 49 markers were significantly associated (P ≤ 0.005) with yield traits. Of which, 38 loci were associated with the same trait when analyzed by using the phenotypic values in multiple environments or using the average values, and 16 loci were associated with at least two traits simultaneously. Some favorable alleles associated with yield traits in multiple environments were discovered, such as Ax2*-null and UMN19*-A362 for reducing plant height, barc21-A220 for increasing spikelet length, gpw2111-A156 for increasing fertile spikelet number per spike, swes65-A120 for increasing spikelet number per spike, VRN-A1*-A1068 for increasing spike number, cfd5-A215 for increasing kernel number per spike, and wmc626-A170 for increasing thousand-kernel weight. These results may provide useful information for marker-assisted selection in wheat breeding programs for yield traits.

Key words: Wheat, Molecular marker, Yield-related traits, Association analysis, Allele

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