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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (07): 1179-1186.doi: 10.3724/SP.J.1006.2013.01179

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Resistance to Verticillium Wilt in Cotton by Reciprocal Grafting and Real-time Quantitative PCR

HAO Jun-Jie1,**,*,HU Yu-Wei1,**,GUO Xiao-Qin2,ZhAO Fu-An3,JIA Xin-He4,GUO Li-Juan1,ZHANG Zhi-Xing1,WANG Qing-Dong5,*   

  1. 1 Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Ministry of Agriculture / Henan Key Laboratory for Control of Crop Diseases and Insect Pests / Plant Protection Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2 College of Chemical and Food Engineering, Zhongzhou University, Zhengzhou 450044, China; 3 Industrial Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 4 Zhengzhou Institute of Agriculture and Forestry Sciences, Zhengzhou 450002, China; 5 Department of Biology Engineering, Zhengzhou University, Zhengzhou 450001, China
  • Received:2012-11-29 Revised:2013-03-11 Online:2013-07-12 Published:2013-04-23
  • Contact: 郝俊杰, E-mail: haojjds@yahoo.com.cn; 王庆东, E-mail: qdwang@zzu.edu.cn

Abstract:

In an attempt to better understand the resistance mechanism of controlling cotton Verticillium wilt by locating responsible for resistance in the plant, reciprocal grafts were made between resistant (Gossypium barbadense, Hai 7124 and Pima 90) and susceptible (G. hirsutum, Jimian 11) genotypes. The grafting combinations were inoculated with Verticillium dahliae. Quantitative monitoring of colonization of V. dahliae in different parts of the plant was analyzed using Real-time quantitative PCR (qPCR). When comparing resistant/susceptible or susceptible/resistant grafted combinations with their resistant and susceptible self-graft checks, respectively, disease index of reciprocal grafts and IC (infection coefficient) values of different parts of the plant were nearly within a range between their corresponding checks, respectively, indicating the pathogens could be transferred through the graft union in reciprocal grafts. The disease index was significantly related with average IC values in different parts of plant in different cotton types. Susceptible/resistant grafted combinations showed basal stem of resistant genotypes had an important role on inhibiting translocation of the pathogens, and resistant/susceptible grafted combinations also showed upper stem above graft union with the resistant ability to V. dahliae in resistant genotypes, which indicated that the resistant genotype G. barbadense has the whole-plant of resistance to V. dahliae. In addition, it seems that all parts of the plant in susceptible genotypes (G. hirsutum) were susceptible to V. dahliae.

Key words: Cotton Verticillium wilt, Reciprocal grafting, Real-time quantitative PCR

[1]Heinz R, Lee S W, Saparno A, Nazar R N, Robb J. Cyclical systemic colonization in Verticillium-infected tomato. Physiol Mol Plant Pathol, 1998, 52: 385–96



[2]Fradin E F, Zhang Z, Juarez Ayala J C, Castroverde C D M, Nazar R N, Robb J, Liu C M, Thomma B P H J. Genetic dissection of verticillium wilt resistance mediated by tomato Ve1. Plant Physiol, 2009, 150: 320–332



[3]Shen Q-Y(沈其益). Basic Studies and Control of Cotton Disease (棉花病害基础研究与防治). Beijing: Science Press, 1992 (in Chinese)



[4]Hao J-J(郝俊杰), Ma Q-X(马奇祥), Liu H-M(刘焕民), Jia X-H(贾新合), Dong Z-D(董中东), Liu S-M(刘书梅), Cui X-W(崔小伟), Zhang Z-X(张志新). Effects of grafting cotton on verticillium wilt resistance, yield and fiber quality of cotton. Sci Agric Sin (中国农业科学), 2010, 43(19): 3974–3980 (in Chinese with English abstract)



[5]Rusk N. Grafting as a potent molecular tool. Nat Methods, 2009, 6: 484



[6]Kim M, Canio W, Kessler S, Sinha N. Developmental changes due to long-distance movement of a homeobox fusion transcript in tomato. Science, 2001, 293: 287–289



[7]Haywood V, Yu T S, Huang N C, Lucas W J. Phloem long-distance trafficking of GIBBERELLIC ACID-INSENSITIVE RNA regulates leaf development. Plant J, 2005, 42: 49–68



[8]Martin A, Adam H, Díaz-Mendoza M, Zurczak M, González-Schain N D, Suárez-López P. Graft-transmissible induction of potato tuberization by the microRNA miR172. Development, 2009, 136: 2873–2881



[9]Park S W, Kaimoyo E, Kumar D, Mosher S, Klessig D F. Methyl salicylate is a critical mobile signal for plant systemic acquired resistance. Science, 2007, 318: 113–116



[10]Bachman M S, Nickell C D. Use of reciprocal grafting to study brown stem rot resistance in soybean. Phytopathology, 1999, 89: 59–63



[11]Mueller D S, Li S, Hartman G L, Pedersen W L. Use of aeroponic chambers and grafting to study partial resistance to Fusarium solani f. sp. glycines in soybean. Plant Dis, 2002, 86: 1223–1226



[12]Ma P(马平). A new inoculation method for Verticillium wilt on cotton and its application in valuating pathogenesis and host resistance. Acta Phytopathol Sin (植物病理学报), 2004, 34(6): 536–541 (in Chinese with English abstract)



[13]Atallah Z K, Bae J, Jansky S H. Multiplex real-time quantitative PCR to detect and quantify Verticillium dahliae colonization in potato lines that differ in response to Verticillium wilt. Phytopathology, 2007, 97: 865–872



[14]Valsesia G, Gobbin D, Patocchi A, Vecchione A, Pertot I, Gessler C. Development of a high-throughput method for quantification of Plasmopara viticola DNA in grapevine leaves by means of quantitative real-time polymerase chain reaction. Phytopathology, 2005, 95: 672–678



[15]Eynck C, Koopmann B, Grunewaldt-Stöcker G, Karlovsky P, von Tiedemann A. Differential interactions of Verticillium longisporum and Verticillium dahliae with Brassica napus detected with molecular and histological techniques. Eur J Plant Pathol, 2007, 118: 259–274



[16]Dong H, Niu Y, Li W, Zhang D. Effects of cotton rootstock on endogenous cytokinins and abscisic acid in xylem sap and leaves in relation to leaf senescence. J Exp Bot, 2008, 59: 1295−1304



[17]Wen T(文婷), Li P-B(李朋波), Jiang S-Q(姜淑琴), Pu J-F(蒲剑锋), Wang Y-M(王玉美), Liu W-X(刘文欣), Hua J-P(华金平). Study on cotton resistance to Verticillium wilt by grafting. Sci Agric Sin (中国农业科学), 2011, 44(24): 5130−5136 (in Chinese with English abstract)



[18]Vallad G E, Subbarao K V. Colonization of resistant and susceptible lettuce cultivars by a green fluorescent protein-tagged isolate of Verticillium dahliae. Phytopathology, 2008, 98: 871−885



[19]Dan H, Ali-Khan S T, Robb J. Use of quantitative PCR diagnostics to identify tolerance and resistance to Verticillium dahliae in potato. Plant Dis, 2001, 85: 700−705



[20]Larsen R C, Vandemark G J, Hughes T J. Development of a real-time polymerase chain reaction assay for quantifying Verticillium alboatrum DNA in resistant and susceptible alfalfa. Phytopathology, 2007, 97: 1519−1525



[21]Mercado-Blancoa J, Collado-Romeroa M, Parrilla-Araujoa S, Rodriguez-Juradoa D, Jimenez-Diaz R M. Quantitative monitoring of colonization of olive genotypes by Verticillium dahliae pathotypes with real-time polymerase chain reaction. Physiol Mol Plant Pathol, 2003, 63: 91–105



[22]Goud J C, Termorshuizen A J. Quality of methods to quantify microsclerotia of Verticillium dahliae in soil. Eur J Plant Pathol, 2003, 109: 523–53
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