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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (09): 1530-1537.doi: 10.3724/SP.J.1006.2013.01530

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of Maize opaque-2 Near-isogenic Lines and Rapid Detection of Lysine Content

CHEN Yan1,SONG Li-Ya2,ZHOU Zhi-Qiang1,ZHANG De-Gui1,YAN Na1,ZHANG Shi-Huang1,LI Xin-Hai1,HAO Zhuan-Fang1,WENG Jian-Feng1,BAI Li1,LI Ming-Shun1,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing 100081, China; 2 Beijing Key Laboratory of Plant Resources Research and Development / Beijing Technology and Business University, Beijing 100048, China
  • Received:2012-12-18 Revised:2013-04-22 Online:2013-09-12 Published:2013-07-09
  • Contact: 李明顺, E-mail: mshunli@aliyun.com
  • About author:李明顺, E-mail: mshunli@aliyun.com

Abstract:

The germplasm of high lysine maize in China is narrow, while the opaque-2 (o2) mutant gene can largely increase the lysine level in maize, so it is important to create opaque-2 near-isogenic lines (o2-NILs) by molecular marker assisted selection (MAS). There are two key problems in creating such lines, one is how to introgress the o2 gene into normal receptor lines correctly, and the other is the fast and convenient way to monitor the lysine content. In our study, the closely linked co-dominant SSR marker phi057 was used to detect the polymorphism between the maize donor and receptor lines, and the specific and co-dominant marker was used to construct o2-NILs. Meanwhile, we tested the lysine content of 18s group of o2-NILs by our own improved dye binding lysine (DBL) method. The results demonstrated that the normal maize inbred lines existed polymorphism, moreover, polymorphism also existed among QPM donors CA339 and Shandong2548, the marker phi057 was effective to construct o2-NILs, using different QPM donors. The lysine content varied in different genetic backgrounds, which was from 0.223% to 0.368% among normal maize inbred lines, and from 0.373% to 0.527% among o2-NILs. The increment of lysine content in o2-NILs ranged from 13% to 74%. We included that the SSR marker phi057 can select the mutant genotype of o2 gene correctly, and the improved DBL method is effective to be used in high lysine maize breeding.

Key words: High lysine maize, Molecular marker assisted selection (MAS), o2-NILs, Dye binding lysine (DBL), Lysine

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