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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1302-1309.doi: 10.3724/SP.J.1006.2010.01302


Molecular Marker-Assisted Selection for Corn o2 Introgression Lines with o16 Gene

ZHANG Wen-Long1,2,4,YANG Wen-Peng2,3,*,CHEN Zhi-Wei1,2,WANG Ming-Chun2,YANG Liu-Qi2,CAI Yi-Lin1   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; 2 Guizhou Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China; 3 Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China; 4 Guizhou Seed Administration Station, Guizhou Agricultural Committee, Guiyang 550001, China
  • Received:2010-02-23 Revised:2010-04-22 Online:2010-08-12 Published:2010-06-11

Abstract: The opaque-2 (o2) mutant is commonly used as a donor to breed high lysine corn. But the highest lysine content of the o2 line as well as the resultant hybrids is about 0.4%. So, it is necessary to increase lysine content for the staple food consumed by human and animals. QCL3021 line carrying opaque-16 (o16) gene is another high lysine mutant. This study was performed to transfer o16 gene into o2 line through hybridization between QCL3021 (donor) and o2 line Taixi 19 (receiver) followed by multiple backcrossing. The foreground selection was performed using simple sequence repeats (SSR) marker umc1066 for o2 gene and umc1141 for o16 gene in every backcrossing and subsequent inbred generation. Selected plants were then screened for recovery of genetic background using SSR markers covering the whole genome. Grain lysine content was determined by dye-binding lysine (DBL) method. Plants with high lysine content and higher genetic background recovery ratio were eventually chosen. In BC2F4 generation, 17 family lines which carry both o2 and o16 genes were identified, with the genetic background recovery ratio of 92%–95%, indicating that the genome of these lines was highly identical with o2 corn line. Lysine content of the selected plants was 0.469%–0.599%, which was an average increase of 122.63% for normal corn,22.33% for the high parent line Taixi 19, and 65.86% for the low parent line QCL3021. This study demonstrated that molecular marker-assisted selection (MAS) for corn o2 introgression lines that were infiltrated with o16 gene was successful in improving grain lysine content. The established procedure can be applied in high lysine corn breeding and genetic improvement.

Key words: Molecular marker-assisted selection, Bakcross, High-lysine mutant, o16, o2, Corn

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