玉米o16基因回交渗入o2系的分子标记辅助选择

doi:10.3724/SP.J.1006.2010.01302

Abstract

Abstract: The opaque-2 (o2) mutant is commonly used as a donor to breed high lysine corn. But the highest lysine content of the o2 line as well as the resultant hybrids is about 0.4%. So, it is necessary to increase lysine content for the staple food consumed by human and animals. QCL3021 line carrying opaque-16 (o16) gene is another high lysine mutant. This study was performed to transfer o16 gene into o2 line through hybridization between QCL3021 (donor) and o2 line Taixi 19 (receiver) followed by multiple backcrossing. The foreground selection was performed using simple sequence repeats (SSR) marker umc1066 for o2 gene and umc1141 for o16 gene in every backcrossing and subsequent inbred generation. Selected plants were then screened for recovery of genetic background using SSR markers covering the whole genome. Grain lysine content was determined by dye-binding lysine (DBL) method. Plants with high lysine content and higher genetic background recovery ratio were eventually chosen. In BC₂F₄ generation, 17 family lines which carry both o2 and o16 genes were identified, with the genetic background recovery ratio of 92%–95%, indicating that the genome of these lines was highly identical with o2 corn line. Lysine content of the selected plants was 0.469%–0.599%, which was an average increase of 122.63% for normal corn,22.33% for the high parent line Taixi 19, and 65.86% for the low parent line QCL3021. This study demonstrated that molecular marker-assisted selection (MAS) for corn o2 introgression lines that were infiltrated with o16 gene was successful in improving grain lysine content. The established procedure can be applied in high lysine corn breeding and genetic improvement.

Key words: Molecular marker-assisted selection, Bakcross, High-lysine mutant, o16, o2, Corn

ZHANG Wen-Long, YANG Wen-Peng, CHEN Zhi-Wei, WANG Ming-Chun, YANG Liu-Qi, CAI Yi-Lin. Molecular Marker-Assisted Selection for Corn o2 Introgression Lines with o16 Gene[J].Acta Agron Sin, 2010, 36(08): 1302-1309.

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Molecular Marker-Assisted Selection for Corn o2 Introgression Lines with o16 Gene

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