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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (06): 984-993.doi: 10.3724/SP.J.1006.2014.00984

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Characterization of Tamyb10 Haplotypes and Their Association with Pre-harvest Sprouting Resistance in a Set of Chinese Red-Grained Wheats

WANG Gen-Ping1,2,**,BI Hui-Hui2,**,SUN Yong-Wei2,WANG Cheng-She1,*,XIA Lan-Qin2,*   

  1. 1 College of Agronomy, Northwest A&F University / State Key Laboratory of Crop Stress Biology in Arid Areas, Shanxi Yangling 712100, China; 2Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-11-13 Revised:2014-03-04 Online:2014-06-12 Published:2014-04-09
  • Contact: 夏兰琴, E-mail: xialanqin@caas.cn; 王成社, E-mail: wangcs2008@126.com

Abstract:

Pre-harvest sprouting (PHS) is one of important factors affecting yield and quality of wheat (Triticum aestivum L.). Generally, red-grained wheat has higher PHS resistance than white wheat. Transcription factor Tamyb10 is a strong candidate for R-1 gene and the expression of its homologues determines wheat seed coat color. In this study, we attempted to analyze the relationship between Tamyb10 haplotype and PHS resistance using amplification result of Tamyb10 specific markers and germination index (GI) evaluated in two locations over two years. In the 119 red-grain Chinese wheat varieties collected from different wheat growth zones in China, seven Tamyb10 haplotypes (Tamyb10-A1, Tamyb10-B1, and Tamyb10-D1 locus) were detected, namely baa, aba, bba, aab, bab, abb, and bbb. Tamyb10 haplotypes were associated with GI value. Tamyb10-D1 had the greatest effect on PHS resistance, followed by Tamyb10-B1 gene, and Tamyb10-A1 had the least effect. Tamyb10 haplotypes distribution showed no obvious regional characteristic, butTamyb10 haplotype bbb correlated with high PHS resistance in the Northeast Spring Wheat Region in China.

Key words: Red-grained wheat, Pre-harvest sprouting, Tamyb10haplotype, Wheat growth zone in China

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