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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (02): 308-317.doi: 10.3724/SP.J.1006.2015.00308

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Diversity Analysis of Rhizosphere Microflora of Wild R. glutinosa Grown in Monocropping for Different Years

WU Lin-Kun1,2,HUANG Wei-Min1,2,**,WANG Juan-Ying1,2,**,WU Hong-Miao1,2,CHEN Jun1,2,QIN Xian-Jin1,2,ZHANG Zhong-Yi2,LIN Wen-Xiong1,2,*   

  1. 1 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Agricultural Agroecological Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2014-04-18 Revised:2014-12-19 Online:2015-02-12 Published:2014-12-29
  • Contact: 林文雄, E-mail: wenxiong181@163.com

Abstract:

The soils sampled from the four different plots, including the newly planted, the two-year monocultured, the wild R. glutinosa and the control without growing R. glutinosa, were used to study the changes in microbial biomass and community composition using phospholipid fatty acid (PLFA) and terminal restriction fragment length polymorphism (T-RFLP) analyses. PLFA analysis indicated that the soil microbial community composition was significantly different among the R. glutinosa with different years of monoculture. Compared with the newly planted soil, the total PLFA content and the ratio of bacteria/fungus in two-year monocultured soil greatly declined. Further analysis by T-RFLP also displayed the distinct differences in rhizospheric bacterial community structure of R. glutinosa. The microbial compositions from the wild and the newly planted R. glutinosa soils tended to be more similar. It was found that the bacteria including Proteobacteria and Firmicutes were predominant in the wild and newly planted R. glutinosa soils. Some beneficial biocontrol bacteria (such as Bacillus, Pseudomonas, etc.) gathered in the rhizosphere of the wild and newly planted R. glutinosa. However, a large number of pathogenic bacteria bred in the rhizosphere of the two-year monocultured R. glutinosa, such as Clostridium sp., Flexibacter polymorphus and Clostridium ghoni, and the number of beneficial bacteria and cellulose degradation bacteria decreased. Furthermore, qRT-PCR analysis verified that the total number of Pseudomonas was much higher in the wild and newly planted R. glutinosa soils than in the two-year monocultured soil. In conclusion, the pathogenic microbes breed seriously in the rhizospheric soil of wild R. glutinosa under the monoculture regime, and yet the number of beneficial bacteria decline, resulting in weakened ability of wild R. glutinosa to resist the diseases so that the two-year monocultured wild R. glutinosa grows abnormally and its yield is decreased drastically.

Key words: Rehmannia glutinosa, PLFA, T-RFLP, Microbial diversity, Plant rhizosphere

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