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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (05): 763-776.doi: 10.3724/SP.J.1006.2017.00763


Effects of Plant Growth Regulators on Fiber Growth and Development in Colored Cotton Ovule Culture in vitro

ZHANG Xiao-Meng**,LIU Song-Jiang**,GONG Wen-Fang,SUN Jun-Ling,PANG Bao-Yin,DU Xiong-Ming*   

  1. Institute of Cotton Research of Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang 455000, China
  • Received:2016-07-14 Revised:2017-01-21 Online:2017-05-12 Published:2017-02-17
  • Contact: Du Xiongming, E-mail: dxm630723@163.com E-mail:18738237057@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31601353).


Naturally colored cotton is an ideal textile raw material for the environmental protection and human health. The ovule of cotton varieties Z1-61, Lyumian CC28 and RT-baixu (CK) was cultured in the media with different concentrations of plant growth regulators (MeJA, SHAM, BR, BRz, FLD, ETH, CoCl2, PAL inhibitor, 4CL inhibitor, Urea, and Chl). After 30 days, the fiber color, fiber length, ovule fresh weight, ovule dry weight and fiber dry weight were determined and analyzed with Duncan's new multiple range method. Under the treatments of salicylhydroxamic, phenylalanine ammonia solution enzyme inhibitor and 4CL inhibitor, the color of Z1-61 and Lyumian CC28 cotton fiber was light, and the higher the concentration of the Salicylhydroxamic acid, the lighter of the cotton fiber color. BRz (2.5 μmol L–1) was conducive to the pigment accumulation in brown cotton fiber, while did not have any significant effect on the pigment appearance of green cotton. Both FLD and CoCl2 made the color of brown cotton fiber lighter, while CoCl2 had little influence on the green color cotton fiber and inhibited the formation of callus. Moreover, salicylhydroxamic acid, BRz, FLD, CoCl2, phenylalanine ammonia solution enzyme inhibitor or 4CL inhibitor decreased the fiber length, ovule fresh weight, fiber dry weight and ovule dry weight. In addition, 5 g L-1 urea or 1 mg L-1 Chl were advantageous to the brown cotton fiber pigment synthesis. In conclusion, the growth regulators tested in this study revealed the correlations among pigments synthesis, hormone, flavonoid metabolism and protein metabolism, which would be useful for developing colored cotton cultivars.

Key words: Colored cotton, Fiber growth, Pigment synthesis, Plant growth regulators

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