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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (3): 411-418.doi: 10.3724/SP.J.1006.2019.84090

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Exogenous MeJA improves cold tolerance of tobacco by inhibiting H2O2 accumulation

Xiao-Han MA,Jie ZHANG,Huan-Wei ZHANG,Biao CHEN,Xin-Yi WEN,Zi-Cheng XU()   

  1. College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2018-07-02 Accepted:2018-10-08 Online:2019-03-12 Published:2019-01-10
  • Contact: Zi-Cheng XU E-mail:zichengxu@126.com
  • Supported by:
    This study was supported by the China National Tobacco Corporation’s Henan Provincial Science and Technology Research Project(201641170024100);This study was supported by the China National Tobacco Corporation’s Henan Provincial Science and Technology Research Project(201641170024099)

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Abstract:

Methyl jasmonate (MeJA) is an elicitor evolving in a variety of physiological and biochemical processes. To explore the effects of exogenous MeJA on tobacco seedlings under low temperature conditions, we used the tobacco variety “Yuyan 10” and sprayed four concentrations (1, 10, 100, 1000 μmol L -1) of MeJA for 3 days on plants. Under low temperature with the treatment under normal temperature as positive and that under low temperature on negative control. The growth indicators, relative electrolyte permeability, photosynthetic pigment content, antioxidant enzyme activity and hormone content of each treatment were measured, showing that the treatment with 10 μmol L -1 methyl jasmonate could reduce the damage of tobacco seedlings caused by low temperature. The effect of H2O2 was then verified by applications of DPI, 10 μmol L -1 MeJA, and DPI+MeJA with the same material and growth period, while low temperature treatment was used as a control. The contents of H2O2, O 2-, CAT, MDA, and ASA-GSH cycle were determined. In conclusion, H2O2 in tobacco plants mainly exists as a poison molecule instead of a second messenger under the treatment of exogenous methyl jasmonate combined with low temperature.

Key words: low temperature stress, tobacco seedlings, MetJA, H2O2 conduction

Table 1

Effect of MeJA on fresh weight and dry weight of shoots and roots in tobacco seedlings under low temperature stress"

处理
Treatment		 鲜重Fresh weight 干重Dry weight
地上部 Aboveground 根 Root 地上部 Aboveground 根 Root
CK 25.52±1.45 a 3.56±0.27 a 3.74±0.52 a 0.30±0.04 a
C0 11.53±1.35 e 1.24±0.08 e 0.59±0.11 d 0.11±0.02 c
C1 12.85±0.52 de 1.13±0.12 de 0.85±0.09 d 0.12±0.05 c
C10 22.58±1.47 b 2.54±0.12 b 2.45±0.08 b 0.23±0.03 b
C100 19.28±0.78 c 2.08±0.04 c 1.84±0.10 c 0.22±0.02 b
C1000 14.03±0.68 d 1.45±0.19 d 0.92±0.09 d 0.11±0.01 c

Fig. 1

Maximum leaf area of each treatment within five days of low temperature treatment Abbreviations are the same as those given in Table 1. Bar superscripted by different letters are significantly different at P < 0.05."

Fig. 2

Relative conductivity and photosynthetic pigment content of each treatment after five days of low temperature treatment Abbreviations are the same as those given in Table 1. Bar superscripted by different letters are significantly different at P < 0.05."

Fig. 3

Hormone content of each treatment after five days of low temperature treatment Abbreviations are the same as those given in Table 1. Bar superscripted by different letters are significantly different at P < 0.05."

Fig. 4

Antioxidant enzyme system activity of each treatment after five days of low temperature treatment Abbreviations are the same as those given in Table 1. Bar superscripted by different letters are significantly different at P < 0.05."

Fig. 5

Active oxygen content, CAT, and MDA contents in treatments under low temperature Bar superscripted by different letters are significantly different at P < 0.05."

Fig. 6

Activities of GR, GSH, APX, ASA in treatments under low temperature Bar superscripted by different letters are significantly different at P < 0.05."

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