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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (3): 317-329.doi: 10.3724/SP.J.1006.2020.92036

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Interactive effects of the photoperiod-/thermo-sensitive genic male sterile genes tms5 and pms3 in rice

Fang-Quan WANG1,2,Fang-Jun FAN1,2,Shi-Jian XIA1,Shou-Yu ZONG1,Tian-Qing ZHENG3,Jun WANG1,2,Wen-Qi LI1,2,Yang XU1,2,Zhi-Hui CHEN1,2,Yan-Jie JIANG1,2,Ya-Jun TAO1,2,Wei-Gong ZHONG1,2,Jie YANG1,2,*()   

  1. 1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement/Jiangsu High Quality Rice R&D Center, Nanjing 210014, Jiangsu, China;
    2. Jiangsu Co-innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China
    3. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-07-08 Accepted:2019-09-26 Online:2020-03-12 Published:2019-10-14
  • Contact: Jie YANG E-mail:yangjie168@aliyun.com
  • Supported by:
    This study was supported by the National Major Project for Developing New GM Crops(2018ZX08001-02B);the Jiangsu Province Natural Science Foundation(BK20171326);and the Jiangsu Province Key Research and Development Program (Modern Agriculture)(BE2018388);and the Jiangsu Province Key Research and Development Program (Modern Agriculture)(BE2017368)

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Abstract:

The tms5 and pms3 are two photoperiod-/thermo-sensitive genic male sterile genes, and their functional sites are clarified. However, the interactive effects of tms5 and pms3 in two-line sterile lines are still unclear. In this study, the functional markers AS-TMS5 and CAPS-PMS3 were designed, according to the functional sites of tms5 and pms3 respectively. The three genotypes of tms5 and pms3 were accurately distinguished by AS-TMS5 and CAPS-PMS3. The relationship of the phenotype and genotype in the F2 population of Pei’ai 64S/9311, Guangzhan 63S/Xianghui 47 and Yueguang S/Ninghui 108 were analyzed respectively. The tms5 was the major gene in Guangzhan 63S and Yueguang S, while pms3 was a non-independent gene in Pei’ai 64S and Yueguang S. By the phenotype and genotype analysis of the F2:3 population of Yueguang S/Ninghui 108, the plants carrying pms3 almost were fertile, while the plants carrying tms5 showed sterility, and had higher transition temperature. Furthermore, the sterility of the plants carrying tms5 and pms3 might have lower transition temperature than those carrying tms5. Pyramiding of tms5 and pms3 provides an efficient scheme to breed photoperiod-/thermo-sensitive genic male sterile lines, which have lower transition temperature and safer production.

Key words: tms5, pms3, functional marker, photoperiod-sensitive male sterility, thermo-sensitive male sterility

Table 1

Primer of function marker for TMS5 and PMS3 genes"

分子标记
Molecular marker		 引物名称
Primer name		 引物序列
Primer sequence (5′-3′)		 产物长度
Product length (bp)
AS-TMS5 T5n-F CCACCGGGTCGGCCGAAGGC 302
T5m-F CCACCGGGTCGGCCGAAGTA
T5-R AGCTCGAAGAGGCGCTCCAC
CAPS-PMS3 PMS3-F GCTTTCCCAGGATGCACATA 410
PMS3-R GTTTGCTCCATTGGTTAGGC

Fig. 1

Temperature chart of experimental plot in August and September of 2015"

Fig. 2

Detection of rice materials (lines) by AS-TMS5 and CAPS-PMS3 marker 1-24 represent Nipponbare, 9311, Nanjing 16, Xianghui 47, Ninghui 108, Nongken 58S, Annong S-1, Zhu 1S, Pei’ai 64S, Guangzhan 63S, Yueguang S, N111S, C815S, Y58S, 509S, Wuxiang S, Shen 08S, 1206S, 1208S, L126S, L128S, Feng 39S, 1892S, and L816S."

Fig. 3

Sequencing analysis of mutant site in TMS5 gene The polymorphic sites present in (a) and (b) are before the CDS of TMS5 gene; (c) site represents the start coding site; (d) site is the functional mutant site."

Fig. 4

Sequencing analysis of mutant site in PMS3 gene"

Table 2

Relationship between genotype and fertility of Yueguang S/Ninghui 108 F2 population"

编号
No.		 AS-
TMS5		 CAPS-
PMS3		 表型
Phenotype		 编号
No.		 AS-
TMS5		 CAPS-
PMS3		 表型
Phenotype		 编号
No.		 AS-
TMS5		 CAPS-
PMS3		 表型
Phenotype
Yueguang S + + S 26 * * F 54 - * F
Ninghui 108 - - F 27 * * F 55 * * F
F1 * * F 28 * - F 56 * * F
1 + * S 29 * * F 57 * + F
2 + * S 30 - + F 58 * * F
3 + * S 31 * * F 59 * * F
4 + * S 32 * * F 60 * * F
5 + + S 33 * - F 61 * + F
6 + * S 34 - - F 62 * + F
7 + * S 35 * - F 63 * * F
8 + - S 36 * * F 64 - * F
9 + * S 37 * * F 65 * - F
10 + * S 38 * * F 66 * + F
11 + * S 39 - * F 67 * + F
12 + + S 40 * * F 68 * * F
13 + + S 41 * - F 69 * + F
14 + - S 42 * - F 70 * - F
15 + * S 43 * * F 71 - + F
16 + + S 44 * * F 72 * - F
17 + - S 45 - + F 73 * * F
18 + + S 46 * + F 74 - - F
19 + * S 47 * - F 75 - * F
20 + * S 48 - - F 76 * + F
21 + * S 49 * * F 77 * - F
22 + * S 50 - * F 78 - * F
23 * - F 51 * - F 79 - - F
24 * - F 52 * - F
25 * * F 53 * * F

Fig. 5

Detection based on AS-TMS5 and CAPS-PMS3 markers for part of Yueguang S/Ninghui 108 F2 population P1: Yueguang S; P2: Ninghui 108; F1: hybrid of Yueguang S/Ninghui 108; 1-45: part of Yueguang S/Ninghui 108 F2 population."

Table 3

Fertility of Yueguang S/Ninghui 108 F2:3 population"

株系
Line		 基因型
Genotype		 第1期 Phase I 第2期 Phase II
抽穗期
Heading date
(month/day)		 总数
Total number		 不育株
No. of sterile plants		 可育株
No. of fertile plants		 抽穗期
Heading date
(month/day)		 总数
Total
number		 不育株
No. of sterile plants		 可育株
No. of fertile plants
S1 TTPP 8/26 11 0 11 9/3 10 0 10
S2 TTPP 8/27 11 0 11 9/9 10 0 10
S3 TTPP 9/11 11 0 11 9/25 10 0 10
S4 TTpp 8/28 11 0 11 9/3 10 0 10
S5 TTpp 9/5 11 0 11 9/14 11 0 11
S6 TTpp 8/20 11 0 11 9/9 11 0 11
S7 TTpp 9/7 11 0 11 9/19 11 0 11
S8 TTpp 9/7 11 1 10 9/22 11 0 11
S9 ttPP 8/28 11 11 0 9/16 11 6 5
S10 ttPP 8/30 11 11 0 9/17 11 2 9
S11 ttPP 9/1 11 11 0 9/17 10 5 5
S12 ttpp 8/23 11 11 0 9/15 10 9 1
S13 ttpp 8/24 11 11 0 9/10 10 10 0
S14 ttpp 8/30 11 11 0 9/13 11 11 0
S15 ttpp 9/10 10 10 0 9/19 9 9 0

Fig. 6

Fertility of F2:3 population from Yueguang S/Ninghui 108 A: the anther morphology and pollen fertility; white bar, 1 mm; black bar, 100 μm; B: the fertility of panicle; bar, 2 cm."

Supplementary table 1

The relationship between genotype and fertility of Pei’ai64S/9311 F2 population"

编号
No.		 CAPS-
PMS3		 表型
Phenotype		 编号
No.		 CAPS-
PMS3		 表型
Phenotype		 编号
No.		 CAPS-
PMS3		 表型
Phenotype
培矮64S Pei’ai 64S + S 20 * F 42 + F
9311 - F 21 * F 43 + F
F1 * F 22 + F 44 + F
1 + S 23 - F 45 * F
2 * S 24 * F 46 * F
3 + S 25 * F 47 + F
4 + S 26 * F 48 + F
5 * S 27 + F 49 * F
6 + S 28 * F 50 * F
7 * S 29 - F 51 - F
8 + S 30 * F 52 - F
9 + S 31 - F 53 * F
10 * F 32 - F 54 * F
11 - F 33 * F 55 * F

Supplementary table 1

The relationship between genotype and fertility of Pei’ai64S/9311 F2 population"

编号
No.		 CAPS-
PMS3		 表型
Phenotype		 编号
No.		 CAPS-
PMS3		 表型
Phenotype		 编号
No.		 CAPS-
PMS3		 表型
Phenotype
12 * F 34 * F 56 * F
13 + F 35 * F 57 * F
14 + F 36 - F 58 + F
15 * F 37 * F 59 - F
16 * F 38 + F 60 - F
17 + F 39 * F 61 * F
18 - F 40 - F 62 + F
19 + F 41 * F

Supplementary table 2

Relationship between genotype and fertility of Guangzhan 63S/Xianghui 47 F2 population"

编号
No.		 AS-
TMS5		 表型
Phenotype		 编号
No.		 AS-
TMS5		 表型
Phenotype		 编号
No.		 AS-
TMS5		 表型
Phenotype
广占63S Guangzhan 63S + S 29 + S 60 * F
湘恢47 Xianghui 47 - F 30 * F 61 * F
F1 * F 31 - F 62 * F
1 + S 32 * F 63 * F
2 + S 33 * F 64 * F
3 + S 34 - F 65 * F
4 + S 35 * F 66 - F
5 + S 36 - F 67 * F
6 + S 37 * F 68 * F
7 + S 38 - F 69 * F
8 + S 39 * F 70 - F
9 + S 40 * F 71 * F
10 + S 41 * F 72 * F
11 + S 42 * F 73 * F
12 + S 43 * F 74 - F
13 + S 44 * F 75 - F
14 + S 45 * F 76 * F
15 + S 46 * F 77 - F
16 + S 47 * F 78 * F
17 - S 48 * F 79 * F
18 + S 49 * F 80 - F
19 + S 50 * F 81 * F
20 + S 51 * F 82 * F
21 + S 52 * F 83 * F
22 + S 53 * F 84 * F
23 + S 54 - F 85 * F
24 + S 55 * F 86 - F
25 + S 56 - F 87 * F
26 + S 57 * F 88 - F
27 + S 58 * F 89 * F
28 + S 59 - F

Supplementary fig. 1

Sunshine time of experimental plot in August and September of 2015"

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[1] CHEN Ri-Rong,ZHOU Yan-Biao,WANG Dai-Jun,ZHAO Xin-Hui,TANG Xiao-Dan,XU Shi-Chong,TANG Qian-Ying,FU Xing-Xue,WANG Kai,LIU Xuan-Ming,YANG Yuan-Zhu. CRISPR/Cas9-mediated editing of the thermo-sensitive genic male-sterile gene TMS5 in rice [J]. Acta Agronomica Sinica, 2020, 46(8): 1157-1165.
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