Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 1999, Vol. 25 ›› Issue (05): 536-540.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Cloning and Modification of 16S Promoter of Tobacco Chloroplast Genome and Production of Transgenic Plants

Shan Song;Zhang Zhonglin;Chen Xi;Liu Chunying;Shen Guifang   

  1. Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081
  • Received:1998-12-23 Revised:1999-05-15 Online:1999-09-12 Published:1999-09-12
  • Contact: Shan Song

Abstract: A Nicotiana tabacum chloroplast transformation vector p16T has been constructed on the basis of a mutated 16S rDNA promoter. It contains not only aad A gene regulated by 16S promoter and psbA teminator,but also two homologous fragments,trnH-psbA and trnK. Using biolistic method,the plasmid p16T h

Key words: Nicotiana tabacum, 16S promoter, Chloroplast expression system, Biolistic transformation

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