Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1425-1434.doi: 10.3724/SP.J.1006.2012.01425

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity and Evolutionary Analysis of Tobacco (Nicotiana tabacum L.) Germplasm Resources Based on ISSR and SRAP Markers

QI Jian-Min1,LIANG Jing-Xia2,CHEN Mei-Xia1,3,XU Jian-Tang1,NIU Xiao-Ping1,ZHOU Dong-Xin4,WANG Tao5,CHEN Shun-Hui6   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science of Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian; 2 Biochemistry Industrial Office of Jining, Jining 272000, China; 3 Department of Biological Engineering of Ningde Normal University, Ningde 352100, China; 4 Fujian Longyan Tobacco Science Institute, Longyan 364000, China; 5Nanping Tobacco Branch Company, Nanping 353000, China; 6 Fujian Tobacco Science Institute, Fuzhou 350003, China
  • Received:2011-12-15 Revised:2012-04-16 Online:2012-08-12 Published:2012-05-11

Abstract: In order to explore on the genetic diversity and genetic evolution among cultivated types and wild species in the genus Nicotiana, 96 tobacco accessions from China and abroad were studied with ISSR and SRAP analysis. Both molecular markers revealed high genetic diversity among species of Nicotiana (genetic similarity = 0.280.58) and low genetic diversity among 91 cultivated tobacco lines (Genetic Similarity = 0.61–0.99).Genetic differentiation index (GST) among species of Nicotiana was 0.83. The order of genetic diversityindex within different cultivated types was cured tobacco>suncured tobacco>burley tobacco>aromatic tobacco. When the similarity coefficient was 0.67 as the cutting line, the cluster results of 96 tobacco accessions based on SRAP and ISSR were as follows: (1) UPGMA analysis showed that all 91 culture varieties clustered into one group separating from N. rustica, N. glutinosa, N. repanda, N. gossei, N. suaveolens. (2) The cured tobacco has close genetic relationship with suncured tobacco, and aromatic tobacco was far away from N. rustica. There was little genetic differentiation between cultivated tobacco lines from China and those from othercountries. (3) The correlation coefficient between genetic distance matrixes based on ISSR and SRAP was 0.68, indicating that the basic results of the two types of markers are consistent. In conclusion, ISSR and SRAP are efficient in generating more accurate information on genetic background, relationship and evolution of tobacco germplasm. The results of this research would be favorable for further practices in tobacco breeding.

Key words: Tobacco (Nicotiana tabacum L.), Sequence-related amplified polymorphism (SRAP), Inter-simple sequence repeat (ISSR), Genetic diversity, Evolution

[1]Coussirat J C. Genetic diversity and varietal identification in the species Nicotiana tabacum by RAPD markers. Annaesddll Tabacum, 1994, 26: 1–7

[2]Coussirat J C. Genetic diversity and varietal identification in Nicotiana tabacum with RAPD markers. Annales du Tabac Section 2, 1994, 26: 1–7

[3]Xu M-H(许明辉), Zheng M-H(郑民慧), Liu Y-Z(刘彦中). Genetic diversity and identification in varieties of Nicotiana tobacum by RAPD marker. Seed (种子), 1998, (5): 23–25 (in Chinese with English abstract)

[4]Yang Y-C(杨友才), Yu S-H(余硕辉), Yin H-Q(尹晗琪). Studies on genetic diversity of 48 tobacco varieties by RAPD analysis. Subtrop Plant Sci (亚热带植物科学), 2008, 37(3): 10–14 (in Chinese with English abstract)

[5]Guo J-P(郭金平), Zhu H-L(朱惠丽), Zhou Y-F(周以飞), Pan D-R(潘大仁), Chen S-H(陈顺辉), Wu S-X(巫升鑫). Genetic diversity and genetic relationship analysis of some flue-cured tobacco germplasms based on RAPD. Chin Tob Sci (中国烟草科学), 2009, 30: 15–18, 24 (in Chinese with English abstract)

[6]Qi J-M(祁建民), Wang T(王涛), Chen S-H(陈顺辉), Zhou D-X(周东新), Fang P-P(方平平), Tao A-F(陶爱芬), Liang J-X(梁景霞), Wu W-R(吴为人). Genetic diversity and genetic relative analysis of tobacco germplasm based on inter-simple sequence repeat (ISSR). Acta Agron Sin (作物学报), 2006, 32: 373–378 (in Chinese with English abstract)

[7]Xiao B-G(肖炳光), Yang B-C(杨本超). Assessment of genetic diversity among tobacco germplasms by ISSR markers. Sci Agric Sin (中国农业科学), 2007, 40(10): 2153–2161 (in Chinese with English abstract)

[8]Fu B(傅冰), Liu D-Q(刘迪秋), Li W-Z(李文正), Chen L-M(陈丽梅), Li K-Z(李昆志). Genetic diversity analysis of 25 tobacco germplasm resources by ISSR molecular marker. J Anhui Agric Sci (安徽农业科学), 2008, 36(3): 898–901 (in Chinese with English abstract)

[9]Liang J-X(梁景霞), Qi J-M(祁建民), Fang P-P(方平平), Wang T(王涛), Chen S-H(陈顺辉), Zhou D-X(周东新), Tao A-F(陶爱芬), Liang K-J(梁康迳), Wu W-R(吴为人). Genetic diversity and genetic relative analysis of tobacco germplasm based on inter-simple sequence repeat (ISSR). Sci Agric Sin (中国农业科学), 2008, 41(1): 286–294 (in Chinese with English abstract)

[10]Ren N, Timko M P. AFLP analysis of genetic polymorphism and evolutionary relationships among cultivated and wild Nicotiana species. Genome, 2001, 44(4): 559–571

[11]Yang Y-C(杨友才), Zhou Q-M(周清明), Yin H-Q(尹晗琪), Zhu L-S(朱列书). Studies on genetic diversity and relationship in tobacco germplasms by AFLP analysis. Sci Agric Sin (中国农业科学), 2006, 39(11): 2194–2199 (in Chinese with English abstract)

[12]Li F-X(李凤霞), Wang W-F(王卫锋), Wang L(王鲁), Cui M-M(崔萌萌), Sun Y-H(孙玉合). Fluorescent AFLP analysis of genetic diversity and evolutionary relatives of Nicotiana genus. Sci Agric Sin (中国农业科学), 2010, 43(12): 2418–2427 (in Chinese with English abstract)

[13]Liu J-F(刘建丰), Wang Z-D(王志德), Liu Y-H(刘艳华), Mou J-M(牟建民). Application of SRAP on analysis of genetic diversity in tobacco germplasms. Chin Tob Sci (中国烟草科学), 2007, 28(5): 49–53 (in Chinese with English abstract)

[14]Liu Y-H(刘艳华), Wang Z-D(王志德), Mu J-M(牟建民), Liu J-F(刘建丰), Dai P-G(戴培刚). Genetic diversity analysis on different tobacco populations. Chin Tob Sci (中国烟草科学), 2009, 30: 19–24 (in Chinese with English abstract)

[15]Ye L-Q(叶兰钦), Xin M-M(辛明明), Du J-K(杜金昆), Xie C-J(解超杰), Li Y-P(李永平), Zhou Q-M(周清明). Genetic diversity revealed by SSR markers of the tobacco cultivars. Chin Agric Sci Bull (中国农学通报), 2009, 25(1): 56–62 (in Chinese with English abstract)

[16]Liu S-C(刘胜传), Liu R-X(刘仁祥), Lei H-M(雷红梅), Chen C-Y(陈春艳). Genetic diversity of some tobacco germplasms by using SSR technique. Guizhou Agric Sci (贵州农业科学), 2009, 37(7): 1–3 (in Chinese with English abstract)

[17]Yang Y-C(杨友才), Zhou Q-M(周清明), Yin H-Q(尹晗琪). Analysis of genetic diversity in tobacco germplasm by RAPDs and AFLPs. J Agric Biotechnol (农业生物技术学报), 2006, 14(4): 585–593 (in Chinese with English abstract)

[18]Tao A-F(陶爱芬), Liu Z-H(刘中华), Qi J-M(祁建民), Zhou D-X(周东新), Wang T(王涛), Chen S-H(陈顺辉). Comparison and analysis of IRAP and ISSR molecular methods used for assessment of genetic diversity in tobacco. J Wuhan Bot Res (武汉植物学研究), 2009, 27(6): 589–594 (in Chinese with English abstract)

[19]Nie Q(聂琼), Liu R-X(刘仁祥). Genetic diversity of 23tobacco germplasm resources by SSR and ISSR analyze. Southwest China J Agric Sci (西南农业学报), 24(1): 15–19 (in Chinese with English abstract)

[20]Liang J-X(梁景霞), Qi J-M(祁建民), Wu W-R(吴为人), Zhou D-X(周东新), Chen S-H(陈顺辉), Wang T(王涛), Tao A-F(陶爱芬). DNA extraction and establishment of SRAP reaction system in tobacco. Acta Tab Sin (中国烟草学报), 2005, 11: 33–38 (in Chinese with English abstract)

[21]Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA, 1979, 76: 5269–5273

[22]Miller M P. Tools for Population Genetic Analysis (TFPGA), version 1.3. Northern Arizona University, Flagstaff, 1997

[23]Mantel N. The detection of disease clustering and a generalized regression approach. Cancer Res, 1967, 27: 209–220

[24]Yeh F C, Yang R C, Boyle T, Ye Z H, Mao J. XPOPGENE, the user friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Canada, 1997

[25]Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA, 1973, 70: 3321–3323

[26]Nei M. Genetic distance between populations. Am Nat, 1972, 106: 283–292

[27]Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 1994, 20: 176–183

[28]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001, 103: 455–461

[29]Wang Y-Y(王元英), Zhou J(周健). Main tobacco assortment blood reasons of China and the United States analysis and tobacco breeding. Acta Tab Sin (中国烟草学报), 1995, (2): 11–22 (in Chinese with English abstract)

[30]Bogani P, Lio P, Intrieri M C, Buiatti M A. physiological and molecular analysis of the genus Nicotiana. Mol Phylogenet Evol, 1997, 7: 62–70

[31]Ren N, Timko M P. AFLP analysis of genetic polymorphism and evolutionary relationships among cultivated and wild Nicotiana species. Genome, 2001, 44: 559–571
[1] XIAO Ying-Ni, YU Yong-Tao, XIE Li-Hua, QI Xi-Tao, LI Chun-Yan, WEN Tian-Xiang, LI Gao-Ke, HU Jian-Guang. Genetic diversity analysis of Chinese fresh corn hybrids using SNP Chips [J]. Acta Agronomica Sinica, 2022, 48(6): 1301-1311.
[2] JIN Min-Shan, QU Rui-Fang, LI Hong-Ying, HAN Yan-Qing, MA Fang-Fang, HAN Yuan-Huai, XING Guo-Fang. Identification of sugar transporter gene family SiSTPs in foxtail millet and its participation in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 825-839.
[3] JIN Rong, JIANG Wei, LIU Ming, ZHAO Peng, ZHANG Qiang-Qiang, LI Tie-Xin, WANG Dan-Feng, FAN Wen-Jing, ZHANG Ai-Jun, TANG Zhong-Hou. Genome-wide characterization and expression analysis of Dof family genes in sweetpotato [J]. Acta Agronomica Sinica, 2022, 48(3): 608-623.
[4] WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[5] ZHAO Jia-Jia, QIAO Ling, WU Bang-Bang, GE Chuan, QIAO Lin-Yi, ZHANG Shu-Wei, YAN Su-Xian, ZHENG Xing-Wei, ZHENG Jun. Seedling root characteristics and drought resistance of wheat in Shanxi province [J]. Acta Agronomica Sinica, 2021, 47(4): 714-727.
[6] LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[7] SUN Qian, ZOU Mei-Ling, ZHANG Chen-Ji, JIANG Si-Rong, Eder Jorge de Oliveira, ZHANG Sheng-Kui, XIA Zhi-Qiang, WANG Wen-Quan, LI You-Zhi. Genetic diversity and population structure analysis by SNP and InDel markers of cassava in Brazil [J]. Acta Agronomica Sinica, 2021, 47(1): 42-49.
[8] Meng-Liang ZHAO,Li-Hui WANG,Yan-Jing REN,Xue-Mei SUN,Zhi-Qiang HOU,Shi-Peng YANG,Li LI,Qi-Wen ZHONG. Genetic diversity of phenotypic traits in 257 Jerusalem artichoke accessions [J]. Acta Agronomica Sinica, 2020, 46(5): 712-724.
[9] Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG. Assessment of genetic diversity by using EST-SSR markers in Lupinus [J]. Acta Agronomica Sinica, 2020, 46(3): 330-340.
[10] MA Yan-Ming, FENG Zhi-Yu, WANG Wei, ZHANG Sheng-Jun, GUO Ying, NI Zhong-Fu, LIU Jie. Genetic diversity analysis of winter wheat landraces and modern bred varieties in Xinjiang based on agronomic traits [J]. Acta Agronomica Sinica, 2020, 46(12): 1997-2007.
[11] MA Yan-Ming, LOU Hong-Yao, CHEN Zhao-Yan, XIAO Jing, XU Lin, NI Zhong-Fu, LIU Jie. Genetic diversity assessment of winter wheat landraces and cultivars in Xinjiang via SNP array analysis [J]. Acta Agronomica Sinica, 2020, 46(10): 1539-1556.
[12] LIU Yi-Ke,ZHU Zhan-Wang,CHEN Ling,ZOU Juan,TONG Han-Wen,ZHU Guang,HE Wei-Jie,ZHANG Yu-Qing,GAO Chun-Bao. Revealing the genetic diversity of wheat varieties (lines) in China based on SNP markers [J]. Acta Agronomica Sinica, 2020, 46(02): 307-314.
[13] YE Wei-Jun,CHEN Sheng-Nan,YANG Yong,ZHANG Li-Ya,TIAN Dong-Feng,ZHANG Lei,ZHOU Bin. Development of SSR markers and genetic diversity analysis in mung bean [J]. Acta Agronomica Sinica, 2019, 45(8): 1176-1188.
[14] WANG Yan-Hua,XIE Ling,YANG Bo,CAO Yan-Ru,LI Jia-Na. Flowering genes in oilseed rape: identification, characterization, evolutionary and expression analysis [J]. Acta Agronomica Sinica, 2019, 45(8): 1137-1145.
[15] Hong-Ju JIAN,Bo YANG,Yang-Yang LI,Hong YANG,Lie-Zhao LIU,Xin-Fu XU,Jia-Na LI. Identification and expression analysis of PEBP gene family in oilseed rape [J]. Acta Agronomica Sinica, 2019, 45(3): 354-364.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!