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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1425-1434.doi: 10.3724/SP.J.1006.2012.01425

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity and Evolutionary Analysis of Tobacco (Nicotiana tabacum L.) Germplasm Resources Based on ISSR and SRAP Markers

QI Jian-Min1,LIANG Jing-Xia2,CHEN Mei-Xia1,3,XU Jian-Tang1,NIU Xiao-Ping1,ZHOU Dong-Xin4,WANG Tao5,CHEN Shun-Hui6   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science of Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian; 2 Biochemistry Industrial Office of Jining, Jining 272000, China; 3 Department of Biological Engineering of Ningde Normal University, Ningde 352100, China; 4 Fujian Longyan Tobacco Science Institute, Longyan 364000, China; 5Nanping Tobacco Branch Company, Nanping 353000, China; 6 Fujian Tobacco Science Institute, Fuzhou 350003, China
  • Received:2011-12-15 Revised:2012-04-16 Online:2012-08-12 Published:2012-05-11

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Abstract: In order to explore on the genetic diversity and genetic evolution among cultivated types and wild species in the genus Nicotiana, 96 tobacco accessions from China and abroad were studied with ISSR and SRAP analysis. Both molecular markers revealed high genetic diversity among species of Nicotiana (genetic similarity = 0.28–0.58) and low genetic diversity among 91 cultivated tobacco lines (Genetic Similarity = 0.61–0.99).Genetic differentiation index (GST) among species of Nicotiana was 0.83. The order of genetic diversityindex within different cultivated types was cured tobacco>suncured tobacco>burley tobacco>aromatic tobacco. When the similarity coefficient was 0.67 as the cutting line, the cluster results of 96 tobacco accessions based on SRAP and ISSR were as follows: (1) UPGMA analysis showed that all 91 culture varieties clustered into one group separating from N. rustica, N. glutinosa, N. repanda, N. gossei, N. suaveolens. (2) The cured tobacco has close genetic relationship with suncured tobacco, and aromatic tobacco was far away from N. rustica. There was little genetic differentiation between cultivated tobacco lines from China and those from othercountries. (3) The correlation coefficient between genetic distance matrixes based on ISSR and SRAP was 0.68, indicating that the basic results of the two types of markers are consistent. In conclusion, ISSR and SRAP are efficient in generating more accurate information on genetic background, relationship and evolution of tobacco germplasm. The results of this research would be favorable for further practices in tobacco breeding.

Key words: Tobacco (Nicotiana tabacum L.), Sequence-related amplified polymorphism (SRAP), Inter-simple sequence repeat (ISSR), Genetic diversity, Evolution

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