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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1416-1424.doi: 10.3724/SP.J.1006.2012.01416

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic Analysis and Molecular Characterization of Two Allelic Mutants of the Dwarf18 Gene in Rice

HOU Lei1,2,YUAN Shou-Jiang3,YIN Liang3,ZHAO Jin-Feng2,WAN Guo-Feng1,ZHANG Wen-Hui1,*,LI Xue-Yong2,*   

  1. 1 School of Life Science, Liaocheng University, Liaocheng 252059, China; 2 National Key Facility for Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Shandong Rice Research Institute, Jinan 250100, China
  • Received:2012-02-02 Revised:2012-04-20 Online:2012-08-12 Published:2012-06-04
  • Contact: 张文会, E-mail: whzhang@lcu.edu.cn, Tel: 135635

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Abstract: We identified two strong dwarf mutants from Nipponbare by EMS mutagenesis, designated as s2-9 and s1-146a. The plant height of these two mutants was 26.3% and 19.2% of that of Nipponbare at the mature stage. They also had wider and dark green leaf at seedling stage. The GA3 treatment of seedling and α-amylase activity analysis in endosperm showed that the mutated gene was involved in GA biosynthesis. Fine mapping showed that the mutant phenotype was tightly linked with the d18 locus. There was a single nucleotide substitution at the 3'-splicing site between the first intron and the 2nd exon in s2-9, whereas there was a single nucleotide substitution in the 2nd exon in s1-146a, which caused a premature stop codon. Semi quantitative RT-PCR revealed that transcript of the D18 gene was up-regulated in s1-146aas compared with WT, but was not detected in s2-9.

Key words: Rice, Dwarf mutant, Dwarf 18, GA, Fine mapping

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