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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1407-1415.doi: 10.3724/SP.J.1006.2012.01407

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of Quantitative Trait Loci Underlying Six Agronomic Traits in Flue-Cured Tobacco (Nicotiana tabacum L.)

TONG Zhi-Jun1,2,JIAO Fang-Chan2,WU Xing-Fu2,WANG Feng-Qing1,CHEN Xue-Jun2,LI Xu-Ying2,3,GAO Yu-Long2,ZHANG Yi-Han2,XIAO Bing-Guang2,*,WU Wei-Ren1,4,*   

  1. 1 Department of Agronomy, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 Yunnan Academy of Tobacco Agricultural Sciences, Yuxi 653100, China; 3 College of Agriculture & Biotechnology, Yunnan Agricultural University, Kunming 650201, China; 4 College of Life Sciences, Fujian Agriculture & Forestry University, Fuzhou 350002, China
  • Received:2012-02-07 Revised:2012-04-20 Online:2012-08-12 Published:2012-06-04

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Abstract: Studies of genetic mapping of quantitative trait loci (QTLs) in tobacco have still been very limited up to date due to the difficulty of molecular marker development and genetic map construction in this species. In this study, with a doubled haploid (DH) population of flue-cured tobacco and a genetic map consisting of 611 SSR markers on 24 linkage groups (LGs) and spanning a total length of 1 882.1 cM constructed based on this population, QTL mapping was performed using the method of composite interval mapping for six agronomic traits related to leaf yield, including plant height (PH), stem girth (SG), internode length (IL), leaf number (LN), length of the largest waist leaf (LWL) and width of the largest waist leaf (WWL). A total of 69 QTLs were detected. Most of the QTLs had small effects, but there were four with relatively large effects, explaining 15–20% of the phenotypic variation in the DH population. The six traits were largely correlated with each other. Consistent with this, many small regions harboring two or more closely linked QTLs of different traits were found in the genome.

Key words: Flue-cured tobacco (Nicotiana tabacum L.), Quantitative trait locus (QTL), Gene

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