Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1416-1424.doi: 10.3724/SP.J.1006.2012.01416

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic Analysis and Molecular Characterization of Two Allelic Mutants of the Dwarf18 Gene in Rice

HOU Lei1,2,YUAN Shou-Jiang3,YIN Liang3,ZHAO Jin-Feng2,WAN Guo-Feng1,ZHANG Wen-Hui1,*,LI Xue-Yong2,*   

  1. 1 School of Life Science, Liaocheng University, Liaocheng 252059, China; 2 National Key Facility for Crop Gene Resource and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Shandong Rice Research Institute, Jinan 250100, China
  • Received:2012-02-02 Revised:2012-04-20 Online:2012-08-12 Published:2012-06-04
  • Contact: 张文会, E-mail: whzhang@lcu.edu.cn, Tel: 135635

PDF

1837

Cited

3

Abstract: We identified two strong dwarf mutants from Nipponbare by EMS mutagenesis, designated as s2-9 and s1-146a. The plant height of these two mutants was 26.3% and 19.2% of that of Nipponbare at the mature stage. They also had wider and dark green leaf at seedling stage. The GA3 treatment of seedling and α-amylase activity analysis in endosperm showed that the mutated gene was involved in GA biosynthesis. Fine mapping showed that the mutant phenotype was tightly linked with the d18 locus. There was a single nucleotide substitution at the 3'-splicing site between the first intron and the 2nd exon in s2-9, whereas there was a single nucleotide substitution in the 2nd exon in s1-146a, which caused a premature stop codon. Semi quantitative RT-PCR revealed that transcript of the D18 gene was up-regulated in s1-146aas compared with WT, but was not detected in s2-9.

Key words: Rice, Dwarf mutant, Dwarf 18, GA, Fine mapping

[1]Davies P J. Plant Hormones: Physiology, Biochemistry and Molecular Biology. Netherlands: Kluwer Academic Publishers, 1995. pp 13–38

[2]Bleecker A B, Schuette J L, Kende H. Anatomical analysis of growth and developmental patterns in the internode of deepwater rice. Planta, 1986, 169: 490–497

[3]Cosgrove D J. Plant cell enlargement and the action of expansins. Bioessays, 1996, 18: 533–540

[4]Cho H T, Kende H. Expression of expansin genes is correlated with growth in deepwater rice. Plant Cell, 1997, 9: 1661–1667

[5]Potter L, Fry S C. Xyloglucan endotransglycosylase activity in pea internodes. Plant Physiol, 1993, 103: 235–241

[6]Uozu S, Tanaka_U M, Kitano H, Hattori K, Matsuoka M. Characterization of XET_related genes of rice. Plant Physiol, 2000, 122: 853–859

[7]Sauter M, Seagull R W, Kende H. Internodal elongation and orientation of cellulose microfibrils and microtubules in deep water rice. Planta, 1993, 190: 354–362

[8]Kinoshita T, Shinbashi N. Identification of dwarf genes and their character expression in the isogenic background. Jpn J Breed, 1982, 32: 219–231

[9]Winkler R G, Helentjaris T. The maize Dwarf3 gene encodes a cytochrome P450-mediated early step in gibberellin biosynthesis. Plant Cell, 1995, 7: 1307–1317

[10]Chiang H H, Hwang I, Goodman H M. Isolation of the Arabidopsis GA4 locus. Plant Cell, 1995, 7: 195–201

[11]Martin D N, Proebsting W M, Hedden P. The SLENDER gene of pea encodes a gibberellin 2-oxidase. Plant Physiol, 1999, 121: 775–781

[12]Thomas S G, Phillips A L, Hedden P. Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation. Proc Natl Acad Sci USA, 1999, 96: 4698–4703

[13]Fujioka S, Yamane H, Spray C R, GAkin P, MacMillan J, Phinney B O, Takahashi, N. Qualitative and quantitative analyses of gibberellins in vegetative shoots of normal, dwarf-1, dwarf-2, dwarf-3, and dwarf-5 seedlings of Zea mays L. Plant Physiol, 1988, 88: 1367–1372

[14]Martin D N, Proebsting W M, Hedden P. Mendel’s dwarfing gene: cDNAs from the Lealleles and function of the expressed proteins. Proc Natl Acad Sci USA, 1997, 94: 8907–8911

[15]Lester D R, Ross J J, Davies P J, Reid J B. Mendel’s stem length gene (Le) encodes a gibberellin 3 β-hydroxylase. Plant Cell, 1997, 9: 1435–1443

[16]Tomoaki S, Koutarou M, Hironori I, Tomoko T, Miyako U T, Kanako I, Masatomo K, Ganesh K A, Shin T, Kiyomi A, Akio M, Hirohiko H, Hidemi K Motoyuki A, Makoto M. An overview of gibberellin metabolism enzyme genes and their related mutants in rice. Plant Physiol, 2004, 134: 1642–1653

[17]Kobayashi M, Sakurai A, Saka H, Takahashi N. Quantitative analysis of endogenous gibberellins in normal and dwarf cultivars of rice. Plant Cell Physiol, 1989, 30: 963–969

[18]Kobayashi M, GAkin P, Spray C R, Phinney B O, MacMillan J. Metabolism of gibberellin A20 to gibberellin A1 by tall and dwarf mutants of Oryza sativa and Arabidopsis thaliana. Plant Physiol, 1994, 106: 1367–1372

[19]Kobayashi M, Kamiya Y, Sakurai A, Saka H, Takahashi N. Metabolism of gibberellins in cell-free extracts of anthers from normal and dwarf rice. Plant Cell Physiol, 1990, 31: 289–293

[20]Hironori I, Miyako U T, Naoki S, Hidemi K, Makoto M, Masatomo K. Cloning and functional analysis of two gibberellin 3β-hydroxylase genes that are differently expressed during the growth of rice. Proc Natl Acad Sci USA, 2001, 98: 8909–8914

[21]Wang H(王慧), Liu Y-Z(刘永柱), Zhang J-G(张建国), Chen Z-Q(陈志强). Genetic analysis of space induced rice dwarf mutant CHA-1 and its response to gibberellic acid (GA3). Chin J Rice Sci (中国水稻科学), 2004, 18(5): 391–395 (in Chinese with English abstract)

[22]Lanahan M B, Ho T H. Slender barley: A constitutive gibberellin-response mutant. Planta, 1988, 175: 107–114

[23]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325

[24]Takeda K. Internode elongation and dwarfism in some gramineous plants. Gamma Field Symp, 1977, 16: 1–18

[25]Miyako U T, Yukiko F, Masatomo K, Motoyuki A, Yukimoto I, Hidemi K, Makoto M. Rice dwarf mutant d1, which is defective in the a subunit of the heterotrimeric G protein, affects gibberellin signal transduction. Proc Natl Acad Sci USA, 2000, 21: 11638–11643

[26]Khoury G, Gruss P. Enhancer elements. Cell, 1983, 33: 313–314
[1] Cui Zhi-Yuan, Qin Chen-Zhan, Liu Xing-Yu, Zhang Hai, Zeng Kang, Huang Guo-Qiang, Xu Jing-Sheng. Interaction between the sugarcane tetraspanin-like protein ScTSPAN18 and 6K2 in response to SCMV infection [J]. Acta Agronomica Sinica, 2026, 52(6): 1618-1630.
[2] Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[3] Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[4] Ma Sheng-Qian, Wang Zhi-Ping, Chen Hao-Tian, Dou Shu-Xian, Zhang Yan, Deng Ai-Xing, Zhang Wei-Jian, Yuan Xiang-Yang, Song Zhen-Wei. Effects of tillage methods and nitrogen application rate on maize yield and soil aggregates in northeastern China under straw returning [J]. Acta Agronomica Sinica, 2026, 52(6): 1802-1816.
[5] Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping. Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment [J]. Acta Agronomica Sinica, 2026, 52(6): 1902-1912.
[6] Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng. Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1728-1742.
[7] Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[8] Zhang Hong-Rong, Wang Fei-Er, Li Pan, Qiu Hai-Long, Zhu Jing, Zhao Lian-Hao, Nan Yun-You, He Wei, Fan Zhi-Long, Hu Fa-Long, Chai Qiang, Yin Wen. Photosynthetic characteristics of 20% reduced irrigation combined with 25% organic substitution for chemical fertilizer in increasing silage maize yield [J]. Acta Agronomica Sinica, 2026, 52(5): 1487-1500.
[9] Han Ya-Xin, He Guan-Hua, Zhang Xiao-Qiong, Zhang Deng-Feng, Li Yong-Xiang, Liu Xu-Yang, Wang Tian-Yu, Li Yu, Zou Hua-Wen, Li Chun-Hui. Identification of maize lateral root density genes resources through integrated RNA-seq and BSA-seq analyses [J]. Acta Agronomica Sinica, 2026, 52(5): 1341-1352.
[10] Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[11] Gu Chun-Miao, Wang Run-Feng, Huang Lu, Liu Hao, Lu Qing, Li Hai-Fen, Li Shao-Xiong, He Shuang-Cheng, Hong Yan-Bin, Chen Xiao-Ping, Tan Bin, Yu Qian-Xia. Genome-wide analysis of the WOX gene family and identification of candidate genes for adventitious shoot regeneration in peanut [J]. Acta Agronomica Sinica, 2026, 52(5): 1326-1340.
[12] Yan An, Jiang Kun-Wei, Wang Rong-Yuan, Tian Lin, Zhang Lu, Wang Yun, Xu Jian-Long. Identification and cloning of SVN7 controlling small vascular bundle number in the rice flag leaf [J]. Acta Agronomica Sinica, 2026, 52(5): 1364-1372.
[13] Wang Yu-Cheng, Zhang Lu, Liu A-Kang, Huang Jian-Liang, Peng Shao-Bing, Yuan Shen. Strategies and prospects for large-scale crop yield improvement based on yield gap [J]. Acta Agronomica Sinica, 2026, 52(5): 1279-1290.
[14] Chen Wei, Wei Wan-Juan, Zhao Qi-Bing, Chang Dong-Wei, Yu Ling-Bo, Zhai Peng-Fei, Feng Zhi-Ming, Chen Zong-Xiang, Ren Yang-Tao, Yang Peng, Liu Hai-Lang, Li Zhen-Fu, Yang Yong-Le, Jin Yan-Gang, Zuo Shi-Min. Developing new germplasm of high-quality and early-maturing rice by editing Hd6 via CRISPR/Cas9 [J]. Acta Agronomica Sinica, 2026, 52(4): 1046-1056.
[15] Cai Hong-Wei, Yu Ai-Zhong, Jiang Ke-Qiang, Wang Peng-Fei, Wang Yu-Long, Huo Jian-Zhe, Pang Xiao-Neng, Yin Bo, Shang Yong-Pan. Key mechanisms underlying the enhancement of sweet maize yield through partial substitution of chemical fertilizers with organic manure in arid irrigation districts [J]. Acta Agronomica Sinica, 2026, 52(4): 1166-1180.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd