绿豆遗传连锁图谱的整合

doi:10.3724/SP.J.1006.2010.00932

Abstract

Abstract:

A high-density genetic linkage map with informative markers is essential for plant genome analysis, including gene mapping, identification of quantitative trait locus (QTL), map-based cloning, and physical map construction. One genetic linkage map of mungbean (Vigna radiata, 2n=2x=22) for the recombinant inbred line population (RIL) derived from an intersubspecific cross between a highly bruchid-susceptible cultivar Berken and a highly bruchid-resistant wild type ACC41 (V. radiata subsp. sublobata) was established. A total of 103 polymorphic SSR markers were screened from 701 pairs markers for density-enhancement of the previous map. Along with other marker data, a new genetic linkage map was constructed by using Mapmaker/Exp 3.0b, with 178 markers, including 96 SSR among which 90 locus from mungbean closely related species, 76 RFLP, four RAPD and two STS, spanning 12 linkage groups, covering total length 1 822.9 cM of the mungbean genome, with an average marker interval distance of 10.30 cM. In the study, we determined the SSR markers transferability of close relatives of mungbean including adzuki bean (Vigna angularis L.), black gram (Vigna mungo L.), common bean(Phaseolus vulgaris), cowpea (V. unguiculata) into mungbean genome. A total of 597 pairs SSR primers were tested and about 65%, 72%, 42%, 30% SSR markers above species respectively were effectively amplified in mungbean, indicating a certain degree of homology between these five genomes, and 97 pairs of polymorphic SSR screened from closely related species can be effectively used in the molecular genetic study of mungbean. Then the new map was compared with the map published for Azuki bean (Vigna angularis,2n=2x=22, n=11) using 32 SSR markers. Most of markers’ order in the two linkage maps was found to be highly conserved. However, chromosome rearrangement was occurred in two genomes after they diverged. In the new map, the major bruchid resistant gene was still mapped on LG I(9) with distances of less than 8.0 cM to the flanking markers, among which, the distance to SSR marker C220 which came from the LG 9 of common bean was less than 3.0 cM. Compared with the previous map, the mew location of the resistance gene is more closely linked with adjacent markers.

Key words: Mungbean, SSR, Genetic linkage map, Comparative mapping, Bruchid resistant gene

DIAO Dan, CHENG Xu-Zhen, WANG Li-Xia, WANG Su-Hua, MA Yan-Ling. Integration of Mungbean(Vigna radiata) Genetic Linkage Map[J].Acta Agron Sin, 2010, 36(06): 932-939.

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Integration of Mungbean(Vigna radiata) Genetic Linkage Map

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