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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (02): 216-223.doi: 10.3724/SP.J.1006.2011.00216

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Characterization of GmAOS Gene and Its Promoter in Soybean (Glycine max)

WU Juan-Juan1,2,WU Qian1,YU De-Yue1,*   

  1. 1 National Center for Soybean Improvement/National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Department of Biochemistry and Molecular Biology, Medical School of Nantong University, Nantong 226001, China
  • Received:2010-07-02 Revised:2010-09-28 Online:2011-02-12 Published:2010-12-15
  • About author:喻德跃, E-mail: dyyu@njau.edu.cn, Tel/Fax: 025-84396410

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Abstract: Allene oxide synthase (AOS) is a major intermediate enzyme in octadecanoid pathway to JA biosynthesis affecting the synthesis and levels of all JA-related compounds in plants, and therefore plays a signi?cant role in plant defense.In this study, a full length cDNA of GmAOS and its promoter were cloned from the soybean (Glycine max) by RT-PCR, RACE, and LA PCR methods. GmAOS cDNA coding 519 amino acids (58.3 kD) with an isoelectric point of 8.97 and two genes copies in the soybean genome coding for GmAOS. Bioinformatics analysis indicated that the N-terminal region of GmAOS displayed features of a typical chloroplast targeting peptide including an enrichment of serine, threonine and tyrosine phosphorylation sites. The length of the promoter was 472 bp, containing several stress-induced elements: GA inducing elements (TAACAA), W-box element which was in response to elicitor-responsive transcription of defense genes, element responsive to salt and pathogen (GAAAAA) and G-box (CACGTG) induced by JA. Jasmonic acid showed a strong inducement of the GmAOS transcript level, expression patterns of GmAOS were explored in two soybean accessions with distinct resistance to cotton worm: XTDD was highly susceptible and HPXQD highly resistant, showing that GmAOS had higher transcript level in HPXQD(HR) than in XTDD(HS). GmAOS transcript level were correlated with soybean material resistance grades. These results suggest GmAOS is likely to be a useful tool for improving self-resistance abality of high plants.

Key words: Soybean, A11ene oxide synthase, Promoter, Clone, Bioinformatics analysis

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