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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 240-244.doi: 10.3724/SP.J.1006.2012.00240


Genetic Analysis and Gene Mapping of a Novel Short Root Hair Mutant in Rice

DING Wo-Na1,HUANG Wei2,NING Yong-Qiang1,ZHU Shi-Hua1   

  1. 1 Laboratory of Plant Molecular Biology, Ningbo University, Ningbo 315211, China; 2 State Key Laboratory of Plant Physiology and Biochemistry, Zhejiang University, Hangzhou 310058, China?
  • Received:2011-06-08 Revised:2011-09-13 Online:2012-02-12 Published:2011-12-01

Abstract: In plants, root hairs are important organs for the uptake of nutrients and water.A rice (Oryza sativa L.) mutant with short root hairs (ossrh3) was isolated from a T-DNA insertion mutant library of rice with Zhonghua 11 background. The elongation of root hairs in the mutant was severely impaired, and other traits were also affected including the plant height, primary root length, lateral root length and number of lateral roots. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene. For mapping the OsSRH3 gene, an F2 population was generated by crossing the mutant ossrh3 with Kasalath wild type. Ultimately by using the published SSR (simple sequence repeat) markers and some newly designed STS (sequence-tagged site) markers, the OsSRH3 was mapped to a 37.7 kb region between the markers S38978 and S39016 on chromosome 1. Within this region, there were eight predicted genes. The study will be helpful for the cloning of OsSRH3 and characterization of the molecular genetic mechanisms of the root hair development in gramineous plants.

[1]Cho H T, Cosgrove D J. Regulation of root hair initiation and expansin gene expression in Arabidopsis. Plant Cell, 2002, 14: 3237–3253

[2]Gilroy S, Jones D L. Through form to function: root hair development and nutrient uptake. Trends Plant Sci, 2000, 5: 56–60

[3]Horn R, Yi K, Menand B, Pernas-Ochoa M, Takeda S, Walker T, Dolan L. Root epidermal development in Arabidopsis. Annu Plant Rev, 2009, 37: 64–82

[4]Libault M, Brechenmacher L, Cheng J, Xu D, Gary S. Root hair systems biology. Trends Plant Sci, 2010, 15: 641–650

[5]Ding W N, Yu Z M, Tong Y L, Huang W, Chen H M, Wu P. A transcription factor with a bHLH domain regulates root hair development in rice. Cell Res, 2009, 19: 1309–1311

[6]Kim C M, Park S H, Je B I, Park S H, Park S J, Piao H L, Eun M Y, Dolan L, Han C D. OsCSLD1, a cellulose synthase-like D1 gene, is required for root hair morphogenesis in rice. Plant Physiol, 2007, 143: 1220–1230

[7]You T, Toyota M, Ichii M, Taketa S. Molecular cloning of a root hairless gene rth1 in rice. Breed Sci, 2009, 59: 13–20

[8]Yu Z M, Kang B, He X W, Lv S L, Bai Y H, Ding W N, Chen M, Cho H, Wu P. Root hair-specific EXPANSINs modulate root hair elongation in rice. Plant J, 2011, 66: 725–734

[9]Won S K, Kumari S, Choi S B, Cho M, Lee S H, Cho H T. Root hair-specific EXPANSIN B genes have been selected for Graminaceae root hairs. Mol Cells, 2010, 30: 369–376

[10]Yoshida S, Forno D A, Cock J H, Gomez K A. Laboratory Manual for Physiological Studies of Rice, 3rd edn. Manila: International Rice Research Institute, 1976. pp 62

[11]Zhang X-Q(张向前), Zou J-S(邹金松), Zhu H-T(朱海涛), Li X-Y(李晓燕), Zeng R-Z(曾瑞珍). Genetic analysis and gene mapping of an early flowering and multi-ovary mutant in rice (Oryza sativa L.). Hereditas (遗传), 2008, 30(10): 1349–1355 (in Chinese with English abstract)

[12]Michelmore R W, Papan I, Kesseli R V. Identification of markers linked to disease resistance genes by bulked segregantanalysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832

[13]Vance C P, Uhde-Stone C, Allan D L. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytol, 2003, 157: 423–447

[14]Wen T J, Schnable P S. Analyses of mutants of three genes that influence root hair development in Zea mays (Gramineae) suggest that root hairs are dispensable. Am J Bot, 1994, 81: 833–842

[15]Wen T J, Hochholdinger F, Sauer M, Bruce W, Schnable P S. The roothairless1 gene of maize encodes a homolog of sec3, which is involved in polar exocytosis. Plant Physiol, 2005, 138: 1637–1643

[16]Hochholdinger F, Wen T J, Zimmermann R, Chimot-Marolle P, Silva O C, Bruce W, Lamkey K R, Wienand U, Schnable P S. The maize (Zea mays L.) roothairless3 gene encodes a putative GPI-anchored, monocot-specific, COBRA-like protein that significantly affects grain yield. Plant J, 2008, 54: 888–898

[17]Engvild K C, Rasmussen S K. Root hair mutants of barley. Barley Genet Newsl, 2004, 34: 13–15

[18]Gahoonia T S, Nielsen N E, Joshi P A, Jahoor A. A roothairless barley mutant for elucidating genetic of root hairs and phosphorus uptake. Plant Soil, 2001, 235: 211–219

[19]Kwasniewski M, Szarejko I. Molecular cloning and characterization of β-expansin gene related to root hair formation in barley. Plant Physiol, 2006, 141: 1149–1158

[20]Kwasniewski M, Janiaka A, Mueller-Roeberb B, Szarejkoa I. Global analysis of the root hair morphogenesis transcriptome reveals new candidate genes involved in root hair formation in barley. J Plant Physiol, 2010, 167: 1076–1083
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