利用表达分析和基因沉默方法研究硫代硫酸硫转移酶基因TaTST与小麦抗白粉病反应的关系

doi:10.3724/SP.J.1006.2012.00231

Abstract

Abstract: Plant thiosulfate sulfurtransferase (TST), which participates in sulfur metabolism, removal of cyanide, generation and removal of reactive oxygen species (ROS), is closely related to plant disease resistance. The wheat TST-encoding gene TaTST was induced by the powdery mildew pathogen fungus Blumeria graminis f.sp. tritici (Bgt) in both theresistant and the susceptible wheat near-isogenic lines. Two expression peaks of TaTST were found from 0 to 48 h after inoculation of Bgt, corresponding to the initial contact and recognition between the host cell and Bgt and the invasion attempt of appressoria and haustoria formation. The two expression peaks were also in agreement with the two oxygen burst reactions. The induced expression level of TaTST was significantly higher in the susceptible line than in the resistant line, which may result in excessive removal of ROS as a response to Bgt infection and so contribute to the process of diseasesusceptibility. TaTST also involved in the process of disease resistance. The method of virus-induced gene silencing (VIGS) was used to silence the TaTST gene of the resistant line. Although TaTST-silencing plants did not produce visible mildew spots or lesions, they showed reduction of resistance to powdery mildew with the increased successful penetration rate and limited elongation of secondary hypha. Decreased density of papilla and delayed H₂O₂ spreading in the Bgt-challenged host cells of the VIGS plants suggest that TaTST possibly affects the Bgt penetration process in resistance response through participating in the ROS accumulation and spread and the papilla formation at early stage of wheat-Bgt interaction.

Key words: Wheat, Blumeria graminis f.sp. tritici (Bgt), Thiosulfate sulfurtransferase (TST), VIGS

HE Yang, YUE Jie-Yu, WANG Hua-Zhong. Gene Expression Profiling and Silencing Reveal the Relationship between TaTST, a Wheat Thiosulfate Sulfurtransferase Gene, and the Resistance Response of Wheat to Powdery Mildew[J].Acta Agron Sin, 2012, 38(02): 231-239.

References

[1]Cipollone R, Ascenzi P, Visca P. Common themes and variations in the rhodanese superfamily. IUBMB Life, 2007, 59: 51–59

[2]Saidu Y. Physicochemical features of rhodanese: A review. Afr J Biotechnol, 2004, 3: 370–374

[3]Papenbrock J, Guretzki S, Henne M. Latest news about the sulfurtransferase protein family of higher plants. Amino Acids, 2011, 41: 43–57

[4]Bartels A, Mock H P, Papenbrock J. Differential expression of Arabidopsis sulfurtransferases under various growth conditions. Plant Physiol Biochem, 2007, 45: 178–187

[5]Papenbrock J, Schmidt A. Characterization of two sulfurtransferase isozymes from Arabidopsis thaliana. Eur J Biochem, 2000, 267: 5571–5579

[6]Mao G H, Wang R G, Guan Y F, Liu Y D, Zhang S Q. Sulfurtransferases 1 and 2 play essential roles in embryo and seed development in Arabidopsis thaliana. Biol Chem, 2011, 286: 7548–7557

[7]Niu J-S(牛吉山), Yu L(于玲), Ma Z-Q(马正强), Chen P-D(陈佩度), Liu D-J(刘大钧). Molecular cloning, characterization and mapping of a rhodanese like gene in wheat. Acta Genet Sin (遗传学报), 2002, 29(3): 266–272 (in Chinese with English abstract)

[8]Walz C, Giavalisco P, Schad M, Juenger M, Klose J, Kehr J. Proteomics of curcurbit phloem exudate reveals a network of defence proteins. Phytochemistry, 2004, 65: 1795–1804

[9]Dubuis P H, Marazzi C, Staedler E, Mauch F. Sulphur deficiency causes a reduction in anti- microbial potential and leads to increased disease susceptibility of oilseed rape. Phytopathology, 2005, 153: 27–36

[10]Bartels A. Functional charactersation of sulfurtransferase proteins in higher plants. PhD Dissertation of Leibniz University, Hannover, Germany, 2006

[11]Vennesland B, Castric P A, Conn E E, Solomonson L P, Volini M, Westley J. Cyanide metabolism. Fed Proc, 1982, 41: 2639–2648

[12]Grossmann K. A role for cyanide, derived from ethylene biosynthesis, in the development of stress symptoms. Physiol Plant, 1996, 97: 772–775

[13]Seo S, Mitsuhara I, Feng J, Iwai T, Hasegawa M, Ohashi Y. Cyanide, a coproduct of plant hormone ethylene biosynthesis, contributes to the resistance of rice to blast fungus. Plant Physiol, 2011, 155: 502–514

[14]Donadio S, Sha?ee A, Hutchinson R. Disruption of a rhodanese like gene results in cysteine auxotrophy in Saccharopolyspora erythraea. J Bacteriol, 1990, 172: 350–360

[15]Rausch T, Wachter A. Sulfur metabolism: a versatile platform for launching defence operations. Trends Plant Sci, 2005, 10: 504–509

[16]Papenbrock J, Bartels A, Hartmann F, Hartmann J, Triulzi T. Reduced sulfur in the plant cell: enzymatic formation and functional roles. In: Sirko A, De Kok L J, Haneklaus S, Hawkesford M J, Rennenberg H, Kaito S, Schnug E, Stulen I, eds. Sulfur metabolism in higher plants. Weikersheim: Markgraf Publishers, 2009. pp 217–219

[17]Nandi D L, Horowitz P M, Westley J. Rhodanese as a thioredoxin oxidase. Int J Biochem Cell Biol, 2000, 32: 465–473

[18]Petty I T, Hunter B G, Wei N, Jackson A O. Infectious barley stripe mosaic virus RNA transcribed in vitro from full-length genomic cDNA clones. Virology, 1989, 171: 342–349

[19]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods, 2001, 25: 402–408

[20]Hückelhoven R, Fodor J, Preis C, Kogel K H. Hypersensitive cell death and papilla formation in barley attacked by the powdery mildew fungus are associated with hydrogen peroxide but not with salicylic acid accumulation. Plant Physiol, 1999, 119: 1251–1260

[21]Caldo R A, Nettleton D, Wise R P. Interaction-dependent gene expression in Mla-specified response to barley powdery mildew. Plant Cell, 2004, 16: 2514–2528

[22]Hückelhoven R, Kogel K H. Reactive oxygen intermediates in plant-microbe interactions: Who is who in powdery mildew resistance? Planta, 2003, 216: 891–902

[23]Liu G S, Sheng X Y, Greenshields D L, Ogieglo A, Kaminskyj S, Selvaraj G, Wei Y D. Profiling of wheat class III peroxidase genes derived from powdery mildew-attacked epidermis reveals distinct sequence-associated expression patterns. Mol Plant Microbe Interact, 2005, 18: 730–741

[24]Harrach B D, Fodor J, Pogány M, Preuss J, Barna B. Antioxidant, ethylene and membrane leakage responses to powdery mildew infection of near-isogenic barley lines with various types of resistance. Eur J Plant Pathol, 2008, 121: 21–33

[25]Caplan J L, Mamillapalli P, Burch-Smith T M, Czymmek K, Dinesh-Kumar S P. Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector. Cell, 2008, 132: 449–462

[26]Feng H Q, Sun K, Li M Q, Li H Y, Li X, Li Y, Wang Y F. The expression, function and regulation of mitochondrial alternative oxidase under biotic stresses. Mol Plant Pathol, 2010, 11: 429–40

[27]Li Z-H(李政红), Gao D-S(高东升), Li X-L(李宪利). The relation between endormancy and changes in two main electron transport pathways of nectarine (Prunus persica var. nectariana) buds. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2006, 32(2): 156–162 (in Chinese with English abstract)

Related Articles 15

[1]	Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[2]	Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun. Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation [J]. Acta Agronomica Sinica, 2026, 52(6): 1830-1846.
[3]	Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[4]	Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[5]	Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[6]	Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[7]	Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei. Genome-wide association study and candidate gene prediction of grain copper content in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1604-1617.
[8]	Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[9]	He Wan-Long, Geng Hong-Wei, Zhang Fei-Fei, Mikereayi·Ababaikere , Luo Zi-Yang, Li Peng-Cheng, Zhou Zhao-Yu, Cheng Yu-Kun. Development of a deep learning-based image recognition system for major wheat diseases [J]. Acta Agronomica Sinica, 2026, 52(5): 1401-1417.
[10]	Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[11]	Hou Si-Yu, Wang Guo-Cui, Wei Jin-Gui, Xie Wei-Xin, Yin Wen, Fan Zhi-Long, Chai Qiang, Hu Fa-Long. Effects of green manure combined with chemical nitrogen fertilizer on dry matter accumulation and yield formation of wheat in arid irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2026, 52(4): 1208-1219.
[12]	Shang Yun-Qiu, Zhao Zhu, Chen Huan, Ding Yong-Gang, Qiao Yu-Qiang, Li Wei, Zhang Xiang-Qian, Cao Cheng-Fu, Du Shi-Zhou. Effects of long-term tillage practices on grain-filling and yield formation in rain-fed wheat [J]. Acta Agronomica Sinica, 2026, 52(4): 1236-1250.
[13]	Qiao Yu-Xin, Li Cheng-Yue, Kang Xiao-Yu, Zhang Xin-Qi, Jia Shao-Hui, Liu Qian, Cao Ya-Li, Shi Xin-Rui, Hao Xing-Yu, Li Ping. Study on the effects of long-term no-tillage straw mulching on wheat yield improvement in dryland areas based on the APSIM model [J]. Acta Agronomica Sinica, 2026, 52(4): 1181-1192.
[14]	Li Can, Zhang Xi-Wei, Zhu Bo-Tao, Zhang Pei-Pei. Functional characterization of wheat GSK kinase TaSK41 and screening for interacting proteins [J]. Acta Agronomica Sinica, 2026, 52(3): 677-687.
[15]	Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Gene Expression Profiling and Silencing Reveal the Relationship between TaTST, a Wheat Thiosulfate Sulfurtransferase Gene, and the Resistance Response of Wheat to Powdery Mildew

PDF

Cited