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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 231-239.doi: 10.3724/SP.J.1006.2012.00231


Gene Expression Profiling and Silencing Reveal the Relationship between TaTST, a Wheat Thiosulfate Sulfurtransferase Gene, and the Resistance Response of Wheat to Powdery Mildew

HE Yang, YUE Jie-Yu, WANG Hua-Zhong   

  1. School of Life Sciences / Tianjin Key Laboratory of Cyto-Genetical & Molecular Regulation, Tianjin Normal University, Tianjin 300387, China
  • Received:2011-08-08 Revised:2011-10-13 Online:2012-02-12 Published:2011-12-01
  • Contact: 王华忠, E-mail: skywhz@mail.tjnu.edu.cn

Abstract: Plant thiosulfate sulfurtransferase (TST), which participates in sulfur metabolism, removal of cyanide, generation and removal of reactive oxygen species (ROS), is closely related to plant disease resistance. The wheat TST-encoding gene TaTST was induced by the powdery mildew pathogen fungus Blumeria graminis f.sp. tritici (Bgt) in both theresistant and the susceptible wheat near-isogenic lines. Two expression peaks of TaTST were found from 0 to 48 h after inoculation of Bgt, corresponding to the initial contact and recognition between the host cell and Bgt and the invasion attempt of appressoria and haustoria formation. The two expression peaks were also in agreement with the two oxygen burst reactions. The induced expression level of TaTST was significantly higher in the susceptible line than in the resistant line, which may result in excessive removal of ROS as a response to Bgt infection and so contribute to the process of diseasesusceptibility. TaTST also involved in the process of disease resistance. The method of virus-induced gene silencing (VIGS) was used to silence the TaTST gene of the resistant line. Although TaTST-silencing plants did not produce visible mildew spots or lesions, they showed reduction of resistance to powdery mildew with the increased successful penetration rate and limited elongation of secondary hypha. Decreased density of papilla and delayed H2O2 spreading in the Bgt-challenged host cells of the VIGS plants suggest that TaTST possibly affects the Bgt penetration process in resistance response through participating in the ROS accumulation and spread and the papilla formation at early stage of wheat-Bgt interaction.

Key words: Wheat, Blumeria graminis f.sp. tritici (Bgt), Thiosulfate sulfurtransferase (TST), VIGS

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