利用表达分析和基因沉默方法研究硫代硫酸硫转移酶基因TaTST与小麦抗白粉病反应的关系

doi:10.3724/SP.J.1006.2012.00231

Abstract

Abstract: Plant thiosulfate sulfurtransferase (TST), which participates in sulfur metabolism, removal of cyanide, generation and removal of reactive oxygen species (ROS), is closely related to plant disease resistance. The wheat TST-encoding gene TaTST was induced by the powdery mildew pathogen fungus Blumeria graminis f.sp. tritici (Bgt) in both theresistant and the susceptible wheat near-isogenic lines. Two expression peaks of TaTST were found from 0 to 48 h after inoculation of Bgt, corresponding to the initial contact and recognition between the host cell and Bgt and the invasion attempt of appressoria and haustoria formation. The two expression peaks were also in agreement with the two oxygen burst reactions. The induced expression level of TaTST was significantly higher in the susceptible line than in the resistant line, which may result in excessive removal of ROS as a response to Bgt infection and so contribute to the process of diseasesusceptibility. TaTST also involved in the process of disease resistance. The method of virus-induced gene silencing (VIGS) was used to silence the TaTST gene of the resistant line. Although TaTST-silencing plants did not produce visible mildew spots or lesions, they showed reduction of resistance to powdery mildew with the increased successful penetration rate and limited elongation of secondary hypha. Decreased density of papilla and delayed H₂O₂ spreading in the Bgt-challenged host cells of the VIGS plants suggest that TaTST possibly affects the Bgt penetration process in resistance response through participating in the ROS accumulation and spread and the papilla formation at early stage of wheat-Bgt interaction.

Key words: Wheat, Blumeria graminis f.sp. tritici (Bgt), Thiosulfate sulfurtransferase (TST), VIGS

HE Yang, YUE Jie-Yu, WANG Hua-Zhong. Gene Expression Profiling and Silencing Reveal the Relationship between TaTST, a Wheat Thiosulfate Sulfurtransferase Gene, and the Resistance Response of Wheat to Powdery Mildew[J].Acta Agron Sin, 2012, 38(02): 231-239.

References

[1]Cipollone R, Ascenzi P, Visca P. Common themes and variations in the rhodanese superfamily. IUBMB Life, 2007, 59: 51–59

[2]Saidu Y. Physicochemical features of rhodanese: A review. Afr J Biotechnol, 2004, 3: 370–374

[3]Papenbrock J, Guretzki S, Henne M. Latest news about the sulfurtransferase protein family of higher plants. Amino Acids, 2011, 41: 43–57

[4]Bartels A, Mock H P, Papenbrock J. Differential expression of Arabidopsis sulfurtransferases under various growth conditions. Plant Physiol Biochem, 2007, 45: 178–187

[5]Papenbrock J, Schmidt A. Characterization of two sulfurtransferase isozymes from Arabidopsis thaliana. Eur J Biochem, 2000, 267: 5571–5579

[6]Mao G H, Wang R G, Guan Y F, Liu Y D, Zhang S Q. Sulfurtransferases 1 and 2 play essential roles in embryo and seed development in Arabidopsis thaliana. Biol Chem, 2011, 286: 7548–7557

[7]Niu J-S(牛吉山), Yu L(于玲), Ma Z-Q(马正强), Chen P-D(陈佩度), Liu D-J(刘大钧). Molecular cloning, characterization and mapping of a rhodanese like gene in wheat. Acta Genet Sin (遗传学报), 2002, 29(3): 266–272 (in Chinese with English abstract)

[8]Walz C, Giavalisco P, Schad M, Juenger M, Klose J, Kehr J. Proteomics of curcurbit phloem exudate reveals a network of defence proteins. Phytochemistry, 2004, 65: 1795–1804

[9]Dubuis P H, Marazzi C, Staedler E, Mauch F. Sulphur deficiency causes a reduction in anti- microbial potential and leads to increased disease susceptibility of oilseed rape. Phytopathology, 2005, 153: 27–36

[10]Bartels A. Functional charactersation of sulfurtransferase proteins in higher plants. PhD Dissertation of Leibniz University, Hannover, Germany, 2006

[11]Vennesland B, Castric P A, Conn E E, Solomonson L P, Volini M, Westley J. Cyanide metabolism. Fed Proc, 1982, 41: 2639–2648

[12]Grossmann K. A role for cyanide, derived from ethylene biosynthesis, in the development of stress symptoms. Physiol Plant, 1996, 97: 772–775

[13]Seo S, Mitsuhara I, Feng J, Iwai T, Hasegawa M, Ohashi Y. Cyanide, a coproduct of plant hormone ethylene biosynthesis, contributes to the resistance of rice to blast fungus. Plant Physiol, 2011, 155: 502–514

[14]Donadio S, Sha?ee A, Hutchinson R. Disruption of a rhodanese like gene results in cysteine auxotrophy in Saccharopolyspora erythraea. J Bacteriol, 1990, 172: 350–360

[15]Rausch T, Wachter A. Sulfur metabolism: a versatile platform for launching defence operations. Trends Plant Sci, 2005, 10: 504–509

[16]Papenbrock J, Bartels A, Hartmann F, Hartmann J, Triulzi T. Reduced sulfur in the plant cell: enzymatic formation and functional roles. In: Sirko A, De Kok L J, Haneklaus S, Hawkesford M J, Rennenberg H, Kaito S, Schnug E, Stulen I, eds. Sulfur metabolism in higher plants. Weikersheim: Markgraf Publishers, 2009. pp 217–219

[17]Nandi D L, Horowitz P M, Westley J. Rhodanese as a thioredoxin oxidase. Int J Biochem Cell Biol, 2000, 32: 465–473

[18]Petty I T, Hunter B G, Wei N, Jackson A O. Infectious barley stripe mosaic virus RNA transcribed in vitro from full-length genomic cDNA clones. Virology, 1989, 171: 342–349

[19]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods, 2001, 25: 402–408

[20]Hückelhoven R, Fodor J, Preis C, Kogel K H. Hypersensitive cell death and papilla formation in barley attacked by the powdery mildew fungus are associated with hydrogen peroxide but not with salicylic acid accumulation. Plant Physiol, 1999, 119: 1251–1260

[21]Caldo R A, Nettleton D, Wise R P. Interaction-dependent gene expression in Mla-specified response to barley powdery mildew. Plant Cell, 2004, 16: 2514–2528

[22]Hückelhoven R, Kogel K H. Reactive oxygen intermediates in plant-microbe interactions: Who is who in powdery mildew resistance? Planta, 2003, 216: 891–902

[23]Liu G S, Sheng X Y, Greenshields D L, Ogieglo A, Kaminskyj S, Selvaraj G, Wei Y D. Profiling of wheat class III peroxidase genes derived from powdery mildew-attacked epidermis reveals distinct sequence-associated expression patterns. Mol Plant Microbe Interact, 2005, 18: 730–741

[24]Harrach B D, Fodor J, Pogány M, Preuss J, Barna B. Antioxidant, ethylene and membrane leakage responses to powdery mildew infection of near-isogenic barley lines with various types of resistance. Eur J Plant Pathol, 2008, 121: 21–33

[25]Caplan J L, Mamillapalli P, Burch-Smith T M, Czymmek K, Dinesh-Kumar S P. Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector. Cell, 2008, 132: 449–462

[26]Feng H Q, Sun K, Li M Q, Li H Y, Li X, Li Y, Wang Y F. The expression, function and regulation of mitochondrial alternative oxidase under biotic stresses. Mol Plant Pathol, 2010, 11: 429–40

[27]Li Z-H(李政红), Gao D-S(高东升), Li X-L(李宪利). The relation between endormancy and changes in two main electron transport pathways of nectarine (Prunus persica var. nectariana) buds. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2006, 32(2): 156–162 (in Chinese with English abstract)

Related Articles 15

[1]	HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2]	GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[3]	LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[4]	FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[5]	FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[6]	LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[7]	XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[8]	YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[9]	WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[10]	CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[11]	MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
[12]	MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
[13]	WEI Yi-Hao, YU Mei-Qin, ZHANG Xiao-Jiao, WANG Lu-Lu, ZHANG Zhi-Yong, MA Xin-Ming, LI Hui-Qing, WANG Xiao-Chun. Alternative splicing analysis of wheat glutamine synthase genes [J]. Acta Agronomica Sinica, 2022, 48(1): 40-47.
[14]	LI Ling-Hong, ZHANG Zhe, CHEN Yong-Ming, YOU Ming-Shan, NI Zhong-Fu, XING Jie-Wen. Transcriptome profiling of glossy1 mutant with glossy glume in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2022, 48(1): 48-62.
[15]	LUO Jiang-Tao, ZHENG Jian-Min, PU Zong-Jun, FAN Chao-Lan, LIU Deng-Cai, HAO Ming. Chromosome transmission in hybrids between tetraploid and hexaploid wheat [J]. Acta Agronomica Sinica, 2021, 47(8): 1427-1436.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Gene Expression Profiling and Silencing Reveal the Relationship between TaTST, a Wheat Thiosulfate Sulfurtransferase Gene, and the Resistance Response of Wheat to Powdery Mildew

PDF

Cited