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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 240-244.doi: 10.3724/SP.J.1006.2012.00240

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Gene Mapping of a Novel Short Root Hair Mutant in Rice

DING Wo-Na1,HUANG Wei2,NING Yong-Qiang1,ZHU Shi-Hua1   

  1. 1 Laboratory of Plant Molecular Biology, Ningbo University, Ningbo 315211, China; 2 State Key Laboratory of Plant Physiology and Biochemistry, Zhejiang University, Hangzhou 310058, China?
  • Received:2011-06-08 Revised:2011-09-13 Online:2012-02-12 Published:2011-12-01

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Abstract: In plants, root hairs are important organs for the uptake of nutrients and water.A rice (Oryza sativa L.) mutant with short root hairs (ossrh3) was isolated from a T-DNA insertion mutant library of rice with Zhonghua 11 background. The elongation of root hairs in the mutant was severely impaired, and other traits were also affected including the plant height, primary root length, lateral root length and number of lateral roots. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene. For mapping the OsSRH3 gene, an F2 population was generated by crossing the mutant ossrh3 with Kasalath wild type. Ultimately by using the published SSR (simple sequence repeat) markers and some newly designed STS (sequence-tagged site) markers, the OsSRH3 was mapped to a 37.7 kb region between the markers S38978 and S39016 on chromosome 1. Within this region, there were eight predicted genes. The study will be helpful for the cloning of OsSRH3 and characterization of the molecular genetic mechanisms of the root hair development in gramineous plants.

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