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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (03): 423-430.doi: 10.3724/SP.J.1006.2013.00423

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Fishing TaSC Interacting Protein in Wheat Using Split Ubiquitin Yeast Two Hybrid System

LIN Fang-Fang,YANG Xu,WU Xiao-Cui,LIU Xiao-Mei,GE Rong-Chao,ZHAO Bao-Cun*   

  1. College of Life Science, Hebei Normal University, Shijiazhuang 050024, China
  • Received:2012-07-09 Revised:2012-11-16 Online:2013-03-12 Published:2013-01-04
  • Contact: 赵宝存, E-mail: baocunzh@126.com, Tel: 0311-80787526

Abstract:

 The TaSC (Triticum asetivum L. salt-tolerance related gene, GenBank accession number AY956330) has been cloned from wheat salt tolerant mutant RH 8706-49, which is induced by high salinity.To fish interacting proteins of this gene, we used TaSC protein as bait to hybrid with the wheat cDNA expression library using the split ubiquitin yeast two hybrid system in this experiment. A novel wheat gene (GenBank accession number AK336035) encoding a protein with unknown function was isolated, which was designated TaSCIP1 (TaSC interaction protein 1).Bimolecular fluorescence complementation (BiFC) experiment confirmed the interaction between proteins TaSCIP1 and TaSC. The separation of TaSC-interacting protein is beneficial to disclose the mechanism of salt tolerance gene TaSC.

Key words: TaSC, Triticum asetivum L., cDNA expression library, Split ubiquitin yeast two-hybrid system, BiFC

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