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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1425-1433.doi: 10.3724/SP.J.1006.2013.01425

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Identification Method of Drought Tolerance and Association Mapping for Sesame (Sesamum indicum L.)

LI Dong-Hua1,LIU Wen-Ping2,ZHANG Yan-Xin1,WANG Lin-Hai1,WEI Wen-Liang1,GAO Yuan1,DING Xia1,WANG Lei1,ZHANG Xiu-Rong1   

  1. 1 Oil Crops Research Institute of Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; 2 Industrial Crop Institute, Shanxi Academy of Agricultural Sciences, Fenyang 032230, China
  • Received:2012-12-31 Revised:2013-04-22 Online:2013-08-12 Published:2013-05-20
  • Contact: 张秀荣, E-mail: zhangxr@oilcrops.cn, Tel: 027-86811836
  • About author:张秀荣, E-mail: zhangxr@oilcrops.cn, Tel: 027-86811836

Abstract:

This research aims at understanding the drought tolerance of sesame germplasm and getting related molecular markers. Ten sesame varieties with different seed coat colors from separate origins were treated with PEG 6000 with different concentrations, and the relative shoot length, relative fresh weight, relative seedling rate and the relative vigor index of the ten sesame varieties were investigated and analyses. The result showed 15% PEG6000 was proper for testing sesame drought tolerance. A comprehensive analysis based on principal component, regression equation and correlation showed that the relative seedling rate could be used as a key indicator to identify drought tolerance of sesame at germination stage. Using the method above, drought tolerance of 216 core collections during germination period was studied, the drought tolerance coefficients of sprout percentage were from 12.15% to 93.52%, the average was 60.74%, the coefficient of variation was 25.22, sprout abundant variation and accordant with normal distribution; together with marker screening, 30 loci were significantly (P<0.05) associated with sesame drought tolerance at germination stage, explaining the phenotypic variation from 1.99% to 4.96%, with an average of 2.84%. So the relative seedling rate is the most suitable and most convenient drought tolerance index, which is suitable for drought tolerance identification of sesame resources.

Key words: Sesame, Germination, PEG 6000, Drought tolerance identification, Association mapping

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