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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (09): 1454-1461.doi: 10.3724/SP.J.1006.2015.01454

• RESEARCH NOTES • Previous Articles    

Screening of Methyl Jasmonate-Insensitive Mutants in Maize

ZHA Xiang-Min1, WANG Hai1, LU Xiao-Duo2, ZHANG Chun-Yi1, HUANG Da-Fang1, *, LANG Zhi-Hong1, *   

  1. 1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 School of Life Sciences, Qilu Normal University, Zhangqiu 250200, China
  • Received:2015-02-09 Online:2015-09-12 Published:2015-09-12

Abstract: The phytohormone jasmonate plays a pivotal role in plant systematic resistance to herbivores attack as a long-distance mobile signal. To demonstrate the Jasmonic acid (JA) signal pathway and the insect-resistant mechanism in maize, we utilized an improved approach to screen the maize mutants associated with JA signal pathway, which will be available for further research. The 6118 lines’ seeds from EMS-mutagenic maize mutants library were cultivated in the medium containing the exogenous MeJA. The different tolerances of the mutants to MeJA were identified by observing the growth of maize roots. When the final concentration of MeJA was 50 µmol L-1, 61 MeJA-insensitive mutants with tolerance Grade I were obtained in the primary screening experiment. When the final concentration of MeJA was 100 µmol L-1 or 200 µmol L-1, 37 and 10 tolerant events were gained in the confirmation experiments, respectively. Then, we analyzed the heredity of M1 seeds tolerant to MeJA. The results showed that the tolerance of the mutants to MeJA was hereditable. The MeJA-insensitive mutants associated with JA regulation pathway are the potential materials for the further studies of JA signal pathway and insect resistant mechanism in maize.

Key words: MeJA/JA, Screening maize mutants library, MeJA-insensitive, Insect attack response

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