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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (09): 1445-1453.doi: 10.3724/SP.J.1006.2015.01445

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Overexpression of Millet Transcription Factor Gene SiNAC45 to Response of Low Potassium Stress and ABA Treatment in Transgenic Arabidopsis

WANG Er-Hui1, 2, **, HU Li-Qin2, **, XUE Fei-Yang1, 2, LI Wei-Wei2, XU Zhao-Shi2, LI Lian-Cheng2, ZHOU Yong-Bin2, MA You-Zhi2, DIAO Xian-Min2, JIA Guan-Qing2, CHEN Ming2, *, MIN Dong-Hong1, *   

  1. 1 College of Agronomy, Northwest A＆F University / State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility For Crop Gene Resource and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crop, Ministry of Agriculture, Beijing 100081, China
  • Received:2015-03-27 Online:2015-09-12 Published:2015-09-12

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Abstract: NAC (nascent polypeptide-associated complex) like transcription factors play important role in plant growth and development, abiotic stress response, and other processes. Currently, few researches reported NAC like transcription factors involving in tolerance to low potassium stress. In this study, we found and researched a NAC like transcription factor gene SiNAC45 on the basis of transcriptome sequence of millet under low potassium stress which had been completed in previous work. The result show that the full-length of SiNAC45 is 1383 bp, encoding 461 amino acids, with molecular weight and isoelectric point of 50.7 kD and 6.92, respectively. There is a conserved NAM domain between 20-100 amino acids of SiNAC45. The phylogenetic tree showed that SiNAC45 belonged to the first subfamily of NAC gene family. The gene expression profile results indicated SiNAC45 mainly expressed in roots and was induced by ABA and low potassium treatment. The protein subcellular localization results of SiNAC45 revealed that it was localized in the nucleus. Gene functional analysis showed that under treatment with different concentrations of potassium, root length and fresh weight of SiNAC45 transgenic Arabidopsis significantly increased compared with those of wild-type Arabidopsis, and there was no significant difference in the number of lateral roots between transgenic and wild-type Arabidopsis, indicating that overexpressing of SiNAC45 in transgenic plants can enhance tolerance to low potassium stress. Expression analysis of downstream gene showed that expression of two important potassium transporter genes AKT1 and HAK1 increased significantly in SiNAC45 transgenic plants, indicating that SiNAC45 affects the tolerance to low potassium stress of plants by regulating the expression of potassium transporter gene. Seed germination test results showed that Arabidopsis carrying SiNAC45 decreased the sensitivity to ABA compared with wild-type Arabidopsis, indicating that SiNAC45 maybe negatively regulate ABA signal pathway.

Key words: Millet, NAC transcription factor, Low potassium stress, ABA response, Gene function analysis

[1] Olsen A N, Ernst H A, Leggio L L, Skriver K. NAC transcription factors: structurally distinct, functionally diverse. Trends Plant Sci , 2005, 10: 79-87.
[2] Ooka H, Satoh K, Doi K, Nagata T, Otomo Y. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana . DNA Res , 2003, 10: 239-247
[3] Souer E, van Houwelingen A, Kloos D, Mol J, Koes R. The no apical meristem gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries. Cell , 1996, 85: 159-170
[4] Le D T, Nishiyama R, Watanabe Y, Mochida K, Yamaguchi- Shinozaki K. Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in soybean during development and dehydration stress. DNA Res , 2011, 18: 263-276
[5] Singh A K, Sharma V, Pal A K, Acharya V, Ahuja P S. Genome-wide organization and expression profiling of the NAC transcription factor familyin potato ( Solanum tuberosum L.). DNA Res , 2013, 20: 403-423
[6] Oliveira T M, Cidade L C, Gesteira A S, Coelho Filho M A, Soares Filho W S. Analysis of the NAC transcription factor gene family in citrus reveals a novel member involved in multiple abiotic stress responses. Tree Genet Genomics , 2011, 7: 1123-1134
[7] Puranik S, Sahu P P, Mandal S N, Parida S K, Prasad M. Comprehensive genome-wide survey, genomic constitution and expression profiling of the NAC transcription factor family in foxtail millet ( Setaria italica L . ) . PLoS One , 2013, 8: 645-694
[8] Su H, Zhang S, Yuan X, Chen C, Wang X. Genome-wide analysis and identification of stress-responsive genes of the NAM-ATAF1, 2-CUC2 transcription factor family in apple. Plant Physiol Biochem , 2013, 71: 11-21
[9] Wang N, Zheng Y, Xin H, Fang L, Li S. Comprehensive analysis of NAC domain transcription factor gene family in Vitis vinifera . Plant Cell Rep , 2013, 32: 61-75
[10] Riechmann J L, Heard J, Martin G, Reuber L, Jiang C Z, Keddie J, Adam L, Pineda O, Ratcliffe O J, Samaha R R, Creelman R, Pilgrim M, Broun P, Zhang J Z, Ghandehari D, Sherman B K, Yu G L. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science , 2000, 290: 2105-2110
[11] Ooka H, Satoh K, Doi K, Nagata T, Otomo Y, Murakami K, Matsubara K, Osato N, Kawai J, Carninci P, Hayashizaki Y, Suzuki K, Kojima K, Takahara Y, Yamamoto K, Kikuchi S. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana . DNA Res , 2003, 10: 239-247
[12] Puranik S, Sahu P P, Srivastava P S, Prasad M. NAC proteins: regulation and role in stress tolerance. Trends Plant Sci , 2012, 17: 1360-1385
[13] Fang Y, You J, Xie K, Xie W, Xiong L. Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice. Mol Genet Genomics , 2008, 280: 547-563
[14] Badis G, Berger M F, Philippakis A A, Talukder S, Gehrke A R. Diversity and complexity in DNA recognition by transcription factors. Science , 2009, 324: 1720-1723
[15] Luscombe N M, Thornton J M. Protein-DNA interactions: amino acid conservation and the effects of mutations on binding specificity. J Mol Biol , 2002, 320: 991-1009
[16] Jensen M K, Kjaersgaard T, Nielsen M M, Galberg P, Petersen K, O’Shea C, Skriver K. The Arabidopsis thaliana NAC transcription factor family: structure-function relationships and determinants of ANAC019 stress signaling. Biochem J , 2010, 426: 183-196
[17] Duval M, Hsieh T F, Kim S Y, Thomas T L. Molecular characterization of AtNAM: a member of the Arabidopsis NAC domain superfamily. Plant Mol Biol , 2002, 50: 237-248
[18] Tran L S, Nakashima K, Sakuma Y, Simpson S D, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis - element in the early responsive to dehydration stress 1 promoter. Plant Cell , 2004, 16: 2481-2498
[19] Hegedus D, Yu M, Baldwin D, Gruber M, Sharpe A, Parkin I, Whitwill S, Lydiate D. Molecular characterization of Brassica napus NAC domain transcriptional activators induced in response to biotic and abiotic stress. Plant Mol Biol , 2003, 53: 383-397
[20] Ashley M K, Grant M, Grabov A. Plant responses to potassium deficiencies: a role for potassium transport proteins. J Exp Bot , 2006, 57: 425-436
[21] Lopez-Bucio J, Cruz-Ramirez A, Herrera-Estrella L. The role of nutrient availability in regulating root architecture. Curr Opin Plant Biol , 2003, 6: 280-287
[22] Gierth M, Maser P, Schroeder J I. The potassium transporter AtHAK5 functions in K + deprivation-induced high-affinity K + uptake and AKT1 K + channel contribution to K + uptake kinetics in Arabidopsis roots. Plant Physiol , 2005, 137: 1105-1114
[23] Shin R, Schachtman D P. Hydrogen peroxide mediates plant root response to nutrient deprivation. Proc Natl Acad Sci USA , 2004, 101: 8827-8832
[24] Fu H H, Luan S. AtKUP1: a dual-affinity K + transporter from Arabidopsis . Plant Cell , 1998, 10: 63-73
[25] Anderson J A, Huprikar S S, Kochian L V, Lucas W J, Gaber R F. Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae. Proc Natl Acad Sci USA , 1992, 89: 3736-3740
[26] Hosy E, Vavasseur A, Mouline K, Dreyer I, Gaymard F, Porée F, Boucherez J, Lebaudy A, Bouchez D, Very A A, Simonneau T, Thibaud J B, Sentenac H. The Arabidopsis outward K + channel GORK is involved in regulation of stomatal movements and plant transpiration. Proc Natl Acad Sci USA , 2003, 100: 5549-5554
[27] Rigas S, Debrosses G, Haralampidis K, Vicente-Agullo F, Feldmann K A, Grabov A, Dolan L, Hatzopoulos P. TRH1 encodes a potassium transporter required for tip growth in Arabidopsis root hairs. Plant Cell , 2001, 13: 139-151
[28] Beehtold N, Ellis J, Pelletier G. In plant Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. Life Sci , 1993, 316: 1194-1199
[29] Yoo S D, Cho Y H, Sheen J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Prot , 2007, 2: 1565-1572
[30] Zhang Z L, Xie Z, Zou X, Casaretto J, Ho T H, Shen Q J. A rice WRKY gene encodes a trans-criptional repressor of the gibberellin signaling pathway in aleurone cells. Plant Physiol , 2004, 134: 1500-1513
[31] Yu D, Chen C, Chen Z. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell , 2001, 13: 1527-1540
[32] Baker S S, Wilhelm K S, Thomashow M F. The 5'-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold, drought and ABA regulated gene expression. Plant Mol Biol , 1994, 24: 701-713
[33] Urao T, Yamaguchi-Shinozaki K, Urao S, Shinozaki K. An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence. Plant Cell , 1993, 5: 1529-1539
[34] Simpson S D, Nakashima K, Narusaka Y, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Two different novel cis -acting elements of erd1, a clpA homologous Arabidopsis gene function in induction by dehydration stress and dark-induced senescence. Plant J , 2003, 33: 259-270
[35] Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi- Shinozaki K. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell , 2003, 15: 63-78
[36] Terzaghi W B, Cashmore A R. Light-regulated transcription. Annu Rev Plant Physiol Plant Mol Biol , 1995, 46: 445-474
[37] Zhang J Z, Creelman R A, Zhu J K. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops. Plant Physiol , 2004, 135: 615-621
[38] Umezawa T, Fujita M, Fujita Y, Yamaguchi-Shinozaki K, Shinozaki K. Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future. Curr Opin Biotechnol , 2006, 17: 113-122
[39] Collinge M, Boller T. Differential induction of two potato genes, Stprx2 and StNAC , in response to infection by Phytophthora infestans and to wounding. Plant Mol Biol , 2001, 46: 521-529
[40] Hegedus D, Yu M, Baldwin D, Gruber M, Sharpe A, Parkin I, Whitwill S, Lydiate D. Molecular characterization of Brassica napus NAC domain transcriptional activators induced in response to biotic and abiotic stress. Plant Mol Biol , 2003, 53: 383-397
[41] Xie Q, Sanz-Burgos H, Guo J A, García C, Gutiérrez. GRAB proteins, novel members of the NAC domain family, isolated by their interaction with a geminivirus protein. Plant Mol Biol , 1999, 39: 647-656
[42] Lu P L, Chen N Z, An R, Su Z, Qi B S, Ren F, Chen J, Wang X C. A novel drought-inducible gene, ATAF1 , encodes a NAC family protein that negatively regulates the expression of stress-responsive genes in Arabidopsis . Plant Mol Biol , 2007, 63: 289-305
[43] Fang Y, You J, Xie K, Xie W, Xiong L. Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice. Mol Genet Genomics , 2008, 280: 547-563
[44] Nakashima K, Tran L S, Van Nguyen D, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi-Shinozaki K. Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice. Plant J , 2007, 51: 617-630
[45] Hu H, You J, Fang Y, Zhu X, Qi Z, Xiong L. Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice. Plant Mol Biol , 2008, 67: 169-181
[46] Sentenac H, Bonneaud N, Minet M, Lacroute F, Salmon J M, Gaymard F, Grignon C. Cloning and expression in yeast of a plant potassium ion transport system. Science , 1992, 256: 663-665
[47] Kim E J, Kwak J M, Uozumi N, Schroeder J I. AtKUP1 : an Arabidopsis gene encoding high-affinity potassium transport activity. Plant Cell , 1998, 10: 51-62
[48] Yamaguchi-Shinozaki K, Shinozaki K. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol , 2006, 57: 781-803
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