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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (09): 1445-1453.doi: 10.3724/SP.J.1006.2015.01445

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Overexpression of Millet Transcription Factor Gene SiNAC45 to Response of Low Potassium Stress and ABA Treatment in Transgenic Arabidopsis

WANG Er-Hui1, 2, **, HU Li-Qin2, **, XUE Fei-Yang1, 2, LI Wei-Wei2, XU Zhao-Shi2, LI Lian-Cheng2, ZHOU Yong-Bin2, MA You-Zhi2, DIAO Xian-Min2, JIA Guan-Qing2, CHEN Ming2, *, MIN Dong-Hong1, *   

  1. 1 College of Agronomy, Northwest A＆F University / State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility For Crop Gene Resource and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crop, Ministry of Agriculture, Beijing 100081, China
  • Received:2015-03-27 Online:2015-09-12 Published:2015-09-12

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Abstract: NAC (nascent polypeptide-associated complex) like transcription factors play important role in plant growth and development, abiotic stress response, and other processes. Currently, few researches reported NAC like transcription factors involving in tolerance to low potassium stress. In this study, we found and researched a NAC like transcription factor gene SiNAC45 on the basis of transcriptome sequence of millet under low potassium stress which had been completed in previous work. The result show that the full-length of SiNAC45 is 1383 bp, encoding 461 amino acids, with molecular weight and isoelectric point of 50.7 kD and 6.92, respectively. There is a conserved NAM domain between 20-100 amino acids of SiNAC45. The phylogenetic tree showed that SiNAC45 belonged to the first subfamily of NAC gene family. The gene expression profile results indicated SiNAC45 mainly expressed in roots and was induced by ABA and low potassium treatment. The protein subcellular localization results of SiNAC45 revealed that it was localized in the nucleus. Gene functional analysis showed that under treatment with different concentrations of potassium, root length and fresh weight of SiNAC45 transgenic Arabidopsis significantly increased compared with those of wild-type Arabidopsis, and there was no significant difference in the number of lateral roots between transgenic and wild-type Arabidopsis, indicating that overexpressing of SiNAC45 in transgenic plants can enhance tolerance to low potassium stress. Expression analysis of downstream gene showed that expression of two important potassium transporter genes AKT1 and HAK1 increased significantly in SiNAC45 transgenic plants, indicating that SiNAC45 affects the tolerance to low potassium stress of plants by regulating the expression of potassium transporter gene. Seed germination test results showed that Arabidopsis carrying SiNAC45 decreased the sensitivity to ABA compared with wild-type Arabidopsis, indicating that SiNAC45 maybe negatively regulate ABA signal pathway.

Key words: Millet, NAC transcription factor, Low potassium stress, ABA response, Gene function analysis

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