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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (02): 222-229.doi: 10.3724/SP.J.1006.2016.00222

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

GhHMGR Gene Function in Ovule Development of Cotton (Gossypium hursutum L.)

MA Fu-Lei,LI De-Mou,LI Zhi,YANG Wei-Juan,ZHOU Xue,YOU Yu,LUO Xiao-Ying*   

  1. Biotechnology Research Center of Southwest University / Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture / Chongqing Key Laboratory of Agricultural Biotechnology, Chongqing 400716, Chin
  • Received:2015-04-10 Revised:2015-09-06 Online:2016-02-12 Published:2015-10-08
  • Contact: 罗小英, E-mail: luoxy@swu.edu.cn E-mail:stickler911@163.com
  • Supported by:

    This research was supported by the Joint Funds of the National Natural Science Foundation of China (31171596) and the Fundamental Research Funds for the Central Universities ( XDJK2014C067, XDJK2014D041).

Abstract:

The constitutive promoter CaMV35S was selected to drive the expression of GhHMGR gene in cotton by using the plant genetic engineering technology. The HMGR contents of ovule (10 DPA) of transgenic lines were detected, and the contents of total sugar, oil and protein as well. Meanwhile, we also performed ovule culture in vitro. The results showed that the expression level of GhHMGR was higher in part organ under light, such as petiole and boll shell than in root and ovule in dark. The GhHMGR gene could partly recover Arabidopsis (Arabidopsis thaliana) hmgr mutant characteristics. Compared with wild-typeWT, the HMGR contents of ovule (10 DPA) in overexpression transgenic lines increased, whereas decreased in suppression expression lines. While the contents of total oil and protein of overexpression transgenic lines increased, and that of total sugar decreased. Meanwhile, the suppression expression lines performed in an opposite trend. The investigation of ovule culture in vitro showed that ovules became deformity after treated with the inhibitor. All of these implied that expression of GhHMGR gene plays a key role in the development of cotton ovule.

Key words: cotton (Gossypium hursutum L.), GhHMGR, ovule, HMGR

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