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Acta Agron Sin ›› 2006, Vol. 32 ›› Issue (05): 734-737.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Expression of Three Members of ACC Synthase Gene Family in Sugarcane Induced by Hormones and Environmental Stress

WANG Ai-Qin; YANG Li-Tao; WANG Zi-Zhang; WEI Yu-Tuo; HE Long-Fei and LI Yang-Rui   

  1. Agricultural College, Guangxi University, Nanning 530005, Guangxi
  • Received:2005-04-16 Revised:1900-01-01 Online:2006-05-12 Published:2006-05-12
  • Contact: LI Yang-Rui

Abstract:

1-aminocyclopropane-1-carboxylic acid synthase is one of the key rate-limiting enzymes for ethylene biosynthesis in higher plants. Based on the cloning of Sc-ACS1, Sc-ACS2 and Sc-ACS3 of ACS gene families, their expressions were tested in the roots and leaves of sugarcane with Sc-ACS1, Sc-ACS2 and Sc-ACS3 as the probes, respectively. The results of Northern blotting analysis showed that the expression of Sc-ACS1 was induced by ethephon in leaves, but not in roots; that of Sc-ACS2 in leaves and roots; and that of Sc-ACS3 in roots, while not in leaves. Under the environmental stresses(cold stress, dark-growing condition and LiCl stress)and hormone inducement (IAA), the mRNA of Sc-ACS1 showed a higher expression while that of Sc-ACS2 maintained low expression level in leaves.

Key words: Saccharum officinarum L. Hybrid, 1-aminocyclopropane-1- carboxylic acid synthase, Gene family, Expression

CLC Number: 

  • S566
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