小麦籽粒蛋白质组分含量及其加工品质的关系

doi:10.3724/SP.J.1006.2009.01306

Abstract

Abstract:

Qualitative and quantitative analyses of glutenin and gliadin contents are important for quality improvement in wheat (Triticum aestivum L.). Although several studies have focused on the relationship between grain processing quality and protein components, there are no consistent conclusions, especially the effects of glutenin and gliadin components on processing quality need to be further studied. In this study, the contents of albumin + globulin, gliadin, HMW-GS, and LMW-GS of 12 wheat cultivars were measured using reversed-phase high-performance liquid chromatography (RP-HPLC). According to glutenin content, gluten protein content, and dough stability time, the 12 cultivars were classified into three groups, namely high-glutenin-content (I), medium -glutenin-content (II), and low-glutenin-content III) groups. Each group consisted of four cultivars. Content of each protein component varied with cultivars, and the content of gluten protein was the main factor to determine total protein content. The content of total protein was positively correlated (P < 0.05) with content of each protein component, and the contents of HMW-GS, LMW-GS, and glutenin had significantly positive correlations with dough development time, stability time, and sedimentation volume. Positive correlations were also observed between HMW/LMW and the development time and stability time of dough. However, the ratio of Gli/HMW-GS was negatively correlated with development time and stability time, and the ratio of Gli/Glu was negatively correlated with dough stability time. High contents of glutenin, HMW-GS, and LMW-GS as well as, large HMW/LMW ratio and small Gli/Glu ratio in wheat grains are favorable for high processing quality in strong-gluten wheat.

Key words: Wheat, Cultivar, Protein composition, Processing quality

SHI Yu,ZHANG Yong-Li,YU Zhen-Wen. Contents of Grain Protein Components and Their Relationships to Processing Quality in Wheat[J].Acta Agron Sin, 2009, 35(7): 1306-1312.

References

[1] Gianibelli M C, Larroque O R, MacRitchie F, Wrigley C W. Biochemical, genetic and molecular characterization of wheat glutenin and its component subunits. Cereal Chem, 2001, 78: 635-646

[2] Khatkar B S, Bell A E, Schofield J D. The dynamic rheological properties of glutens and gluten subfractions from wheats of good and poor bread making quality. J Cereal Sci, 1995, 22: 29-44

[3] Liu L(刘丽), Zhou Y(周阳), He Z-H(何中虎), Wang D-S(王德森), Zhang Y(张艳), Peña R J. Effect of allelic variation in HMW and LMW glutenin subunits on the processing quality in common wheat. Sci Agric Sin (中国农业科学), 2004, 37(1): 8-14 (in Chinese with English abstract)

[4] Song J-M(宋建民), Liu A-F(刘爱峰), Wu X-Y(吴祥云), Liu J-J(刘建军), Zhao Z-D(赵振东), Liu G-T(刘广田). Composition and content of high-molecular-weight glutenin subunits and their relations with wheat quality. Sci Agric Sin (中国农业科学),2003, 36(2): 128-133 (in Chinese with English abstract)

[5] Antes S, Wieser H. Effects of high and low molecular weight glutenin subunits on rheological dough properties and breadmaking quality of wheat. Cereal Chem, 2001, 78: 157-159

[6] Wang Y-F(王月福), Yu Z-W(于振文), Li S-X(李尚霞), Yu S-L(余松烈). Effects of nitrogen application amount on content of protein components and processing quality of wheat grain. Sci Agric Sin (中国农业科学), 2002, 35(9): 1071-1078 (in Chinese with English abstract)

[7] Zhu J-B(朱金宝), Liu G-T(刘广田), Zhang S-Z(张树榛), Sun H(孙辉). High and low molecular subunits of glutenin and their relationships with wheat quality. Sci Agric Sin (中国农业科学), 1996, 29(1): 34-39 (in Chinese with English abstract)

[8] Van Lonkhuijsen H J, Hamer R J, Schreuder C. Influence of specific gliadins on the breadmaking quality of wheat. Cereal Chem, 1992, 69: 174-177

[9] Zhang P-P(张平平), Chen D-S(陈东升), Zhang Y(张勇), Xia X-C(夏先春), He Z-H(何中虎). Gliadin composition and their effects on quality properties in spring wheat. Acta Agron Sin (作物学报), 2006, 32(12): 1796-1801 (in Chinese with English abstract)

[10] Yang X-J(杨学举), Lu S-Y(卢少源), Zhang R-Z(张荣芝). Relations between protein groups and bread-baking quality characters of wheat. J Chin Cereals Oils Assoc (中国粮油学报), 1999, 14(1): 1-5 (in Chinese with English abstract)

[11] Wieser H, Antes S, Seilmeier W. Quantitative determination of gluten protein types in wheat flour by reversed-phase high-performance liquid chromatography. Cereal Chem, 1998, 75: 644-650

[12] Zhang P-P(张平平), Zhang Y(张勇), Xia X-C(夏先春), He Z-H(何中虎).Protocol establishment of reversed-phase high-performance liquid chromatography (RP-HPLC) for analyzing wheat gluten protein. Sci Agric Sin (中国农业科学), 2007, 40(5): 1002-1009 (in Chinese with English abstract)

[13] Tang J-W(唐建卫), Liu J-J(刘建军), Zhang P-P(张平平), Zhang Y(张艳), Xiao Y-G(肖永贵), Qu Y-Y(曲延英), Zhang Y(张勇), He Z-H(何中虎). Effects of gluten protein fractions on dough property and products quality in common wheat. Sci Agric Sin (中国农业科学), 2008, 41(10): 2937-2946

[14] He Z-F(何照范). Analysis Techniques for Grain Quality of Cereals and Oils (粮油籽粒品质及分析技术). Beijing: Agriculture Press, 1985. pp 37-41 (in Chinese)

[15] Elpidio P, Angeles B, Consuelo S, Nicolás J. Relationship between common wheat (Triticum aestivum L.) gluten proteins and dough rheological properties. Euphytica, 2005, 143: 169-177

[16] Eckert R, Berghofer E, Ciclitira P J, Chirdo F, Denenry-Papini S, Ellis H J, Ferranti P, Goodwin P, Immer U, Mamone G, Méndez E, Mothes T, Novalin S, Osman A, Rumbo M, Stern M, Thorell L, Whim A, Wieser H. Towards a new gliadin reference material-isolation and characterization. J Cereal Sci, 2006, 43: 331-341

[17] Wieser H, Kieffer R. Correlations of the amount of gluten protein types to the technological properties of wheat flours determined on a micro-scale. J Cereal Sci, 2001, 34: 19-27

[18] Tang J-W(唐建卫), Liu J-J(刘建军), Zhang P-P(张平平), Zhang Y(张艳), Li H-S(李豪圣), Zhao Z-D(赵振东), Qu Y-Y(曲延英), He Z-H(何中虎). Dough properties and loaf quality stability in wheat cultivar Jimai 20 and their relationship with protein fractions. Acta Agron Sin (作物学报), 2007, 33(11): 1788-1793 (in Chinese with English abstract)

[19] Zhang P P, He Z H, Zhang Y, Xia X C, Chen D S, Zhang Y. Association between % SDS-unextractable polymeric protein (%UPP) and end-use quality in Chinese bread wheat cultivars. Cereal Chem, 2008, 85: 696-700

[20] Wieser H, Zimmermann G. Importance of amounts and proportions of high molecular weight subunits of glutenin for wheat quality. Eur Food Res Technol,2000, 210: 324-330

[21] Zhang P P, He Z H, Zhang Y, Xia X C, Liu J J, Yan J, Zhang Y. Pan bread and Chinese white salted noodle qualities of Chinese winter wheat cultivars and their relationship with gluten protein fractions, Cereal Chem, 2007, 84: 370-378

[22] Lukow O M, Forsyth S A, Payne P I. Over-production of HMW glutenin subunits coded on chromosome 1B in common wheat, Triticum aestivum. J Genet Breed, 1992, 46: 187-191

[23] Zhang P P, He Z H, Chen D S, Zhang Y, Oscar R L, Xia X C. Contribution of common wheat protein fractions to dough properties and quality of northern-style Chinese steamed bread. J Cereal Sci, 2007, 46: 1-10

[24] Scanlon M G, Ng P K W, Lawless D E, Bushuk W. Suitability of reversed-phase high- performance liquid chromatographic separation of wheat proteins for long-term statistical assessment of breadmaking quality. Cereal Chem, 1990, 67: 395-399
Weegels P L, Hamer R J, Schofield J D. Functional properties of wheat glutenin.J Cereal Sci, 1996, 23: 1-18

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