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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (1): 184-190.doi: 10.3724/SP.J.1006.2010.00184

• RESEARCH ACTIVITIES • Previous Articles    

Observation of Chloroplast Ultrastructure of Zebra Leaf in Rice Mutant b411

QIU Yi-Lan1,LI Hong1,2,PENG Ke-Qin3,LIU Zhu-Li1,CHEN Song1,LIU Ru-Shi1,LIANG Man-Zhong1,CHEN Liang-Bi1*   

  1. 1 College of Life Sciences, Hunan Normal University, Changsha 410081, China; 2 Ningxiang First Senior Middle School, Ningxiang 410600, China; 3.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
  • Received:2009-06-11 Revised:2009-09-04 Online:2010-01-12 Published:2009-11-17
  • Contact: CHEN Liang-Bi, E-mail: chenliangbi@126.com; Tel: 0731-8872617

Abstract:

A rice zebra leaf mutant B411 was examined using transmission electron microscopy technique to study the chloroplast ultrastructure during development of zebra leaf. The results showed that there was a distinct difference between green and yellow zones of the same leaf when the leaf was enwrapped in the sheath. Chloroplasts were spindle-shaped with thylakoid membranes neatly arranged along the longitudinal direction in the green zone, however, chloroplasts in the yellow zone were oval-shaped with thylakoid membranes ranked irregularly. Along with zebra leaves shooting out of the sheath, difference between yellow zone and green zone in the same leaf became more and more evident, in the green zone, thylakoid membranes became rich, and piled up to be the grana. Nevertheless, thylakoid membrane fragmented, internal structure of chloroplast disaggregated seriously and had vesicle structures with high electron density in the yellow zone. With regreening in the yellow zone of zebra leaf, the shape of chloroplast in the yellow zone became spindle, and thylakoid membrane system reconstructed and gradually became rich, orderly arranging along with the long axis of the chloroplast, and some starch granules formed in the chloroplast which indicated that chloroplasts resumed the capacity of photosynthesis.

Key words: Rice, Zebra leaf, Chloroplast, Ultrastructure

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