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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (2): 202-210.doi: 10.3724/SP.J.1006.2010.00202

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Establishment of DNA Fingerprint ID in Sugarcane Cultivars in Yunnan, China

LIU Xin-Long1,2,MA Li1,2,CHEN Xue-Kuan1,2,YING Xiong-Mei1,2,CAI Qing1,3,LIU Jia-Yong1,2,WU Cai-Wen1,2,*   

  1. 1 Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661600, China; 2 Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661600, China; 3 Biotechnology & Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China
  • Received:2009-07-03 Revised:2009-09-08 Online:2010-02-10 Published:2009-12-21
  • Contact: WU Cai-Wen, E-mail: gksky_wcw@163.com E-mail:lxlgood868@163.com

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Abstract:

To well evaluate and use the cultivars, we should identify them scientifically and accurately by DNA molecular markers. In this paper, 27 cultivars developed by two breeding institutes in Yunnan province were analyzed with SSR marker. Eight pairs of core SSR primers selected from about 120 pairs of SSR primers offered by the International Sugarcane Microsatellite Consortium made up the core primers for DNA fingerprint. A total of 129 bands were acquired by PAGE with the core primers, 123 of which were polymorphic bands, accounting for percentage of polymorphic band (PPB) was 95.35%, and the mean value of polymorphism information content(PIC) reached 0.9445; the genetic similarity coefficient of the cultivars was 0.269–0.767. SMC1047HA and MSSCIR21 with high PIC value could be used to distinguish all cultivars, which were the most efficient single primers. The result of evaluating different primer combinations from eight core primers indicated that MSSCIR36/MSSCIR21, MSSCIR16/MSSCIR36, and MSSCIR36/SMC336BS were very efficient in identifying these Yunnan cultivars, and their similar coefficients were lower than those of other primer combinations. At the same time, three primer combinations were validated with ten main released cultivars. The result showed MSSCIR16/MSSCIR36 was the optimum primer combination, which can be used in constructing DNA fingerprint ID of cultivars. The DNA fingerprint ID was set up, including serial number of National Nursery of Sugarcane Germplasm Resources (NNSGR), country& region code, breeding institute, core primer name and SSR marker data, which not only consists of the important information of cultivars, but also helps researchers to identify cultivars efficiently. At the same time, it can provide reliable scientific evidence for the protection of intellectual property right for these cultivars.

Key words: Yunnan sugarcane culticar, SSR, DNA, Fingerprint, ID

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