Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (2): 211-216.doi: 10.3724/SP.J.1006.2010.00211

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Molecular Mapping of Stripe Leaf Mutant st(t) in Rice (Oryza sativa L.)

SANG Xian-Chun,XU Fang-Fang,LING Ying-Hua,ZHAO Fang-Ming,YANG Zheng-Lin,TANG Yan-Qiang,TIAN Xiao-Qing,LI Yun-Feng,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Key Laboratory of Southwest Crop Genetic Improvement and Breeding, Ministry of Agriculture, Chongqing 400716, China
  • Received:2009-07-09 Revised:2009-10-08 Online:2010-02-10 Published:2009-12-21
  • Contact: HE Guang-hua,E-mail: hegh@swu.edu.cn E-mail:sangxianchun@163.com

PDF

2182

Cited

15

Abstract:

A stripe leaf mutant temporarily designated as st(t) was discovered in the progeny of an excellent indica restorer line Jinhui10 seed by treatment of EMS (ethyl methane sulfonate) in Rice Research Institute of Southwest University. The st(t) mutant displayed the variegated leaves at three-leaf stage, the variegation developed the white irregular line stripe from elongating stage to the maturation. Compared with the wild type, the mutant decreased the chlorophyll content and increased the carotenoid content significantly. Cells structure was detected by Transmission Electron Microscopic (TEM), and the results indicated that there was no difference between the wild type and the green parts of the st(t), both of them could develop normal chloroplast; in the white parts of the st(t), cells developed abnormally contained irregular stromal lamellae, aberrant thylakoids and more osmiophilic granules. Genetic analysis suggested that the mutational characters were controlled by one nuclear recessive gene. Xinong1A was crossed with the st(t) and 1 500 mutants from the F2 population were used for gene mapping. Finally, St(t) gene was mapped between SSR marker RM19745 and RM19762 on the chromosome 6, the genetic distances were 0.07 cM and 0.27 cM respectively and the physical distance was 345 kb according to indica rice 9311. This result provides a foundation of map-based cloning of St(t) gene as well as its molecular marker-assisted breeding.

Key words: Stripe leaf, Rice(Oryza sativa L.), Chloroplast, Gene mapping

[1]         Rüdiger W. Chlorophyll metabolism: from outer space down to the molecular level. Phytochemistry, 1997, 46: 1151–1167
[2]         BollivarDW.Recent advances in chlorophyll biosynthesis. Photosynth Res, 2006, 90: 173–194
[3]         Tanaka A, Tanaka R. Chlorophyll metabolism. Curr Opin Plant Biol, 2006, 9: 248–255
[4]         Kurata N, Miyoshi K, Nonomura K I, Yamazaki Y, Ito Y. Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiol, 2005, 46: 48–62
[5]         Wu Z M, Zhang X, He B, Diao L P, Sheng S L, Wang J L, Guo X P, Su N, Wang L F, Jiang L, Wang C M, Zhai H Q, Wan J M. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007,145: 29−40
[6]         Chen T, Zhang Y D, Zhao L, Zhu Z, Lin J, Zhang S B, Wang C L. Fine mapping and candidate gene analysis of a green-revertible albino gene gra(t) J Genet Genomics, 2009,36(2): 117−123in rice.
[7]         Liu W Z, FuY P, HuG C, Si H M, ZhuL, Wu C, SunZ X. Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.). Planta, 2007, 226: 785–795
[8]         Zhang H T, Li J J, YooJ H, Yoo S C,ChoS H,Koh H J, Seo H S,Paek N C. Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant Mol Biol, 2006, 62: 325–337
[9]         Kensuke K, Hisayo K, Hikaru S, Koh I. Characterization of a zebra mutant of rice with increased susceptibility to light stress. Plant Cell Physiol, 2000, 41(2): 158−164
[10]      Sanchez A C, Khush G S. Chromosomal location of some marker genes in rice using the primary trisomics. J Hered, 1994, 85: 297−300
[11]      Li H C, Qian Q, Wang Y, Li X B, Zhu L H, Xu J C. Characterization and mapping of a white panicle mutant gene in rice. Chin Sci Bull, 2003, 48(5): 457−459
[12]      Kensuke K, Akiko M, Mitsuo N, Koh I. A virescent gene V1 determines the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice. Plant J, 1997, 12: 1241−1250
[13]      Sugimoto H, Kusumi K, Tozawa Y, Yazaki J, Kishimoto N, Kikuchi S, Iba K. The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation. Plant Cell Physiol, 2004, 45: 985−996
[14]      Sugimoto H, Kusumi K, Noguchi K, Yano M, Yoshimura A, Iba K. The rice nuclear gene, VIRESCENT 2, is essential for chloroplast development and encodes a novel type of guanylate kinase targeted to plastids and mitochondria. Plant J, 2007, 52: 512−527
[15]      Li N(李娜), Chu H-W(储黄伟), Wen T-Q(文铁桥), Zhang D-B(张大兵). Genetic analysis and mapping of the rice white midrib mutantOswm. Acta Agric Shanghai (上海农业学报), 2007, 23(1): 1−4 (in Chinese with English abstract)
[16]      Wellburn A R. The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol, 1994, 144: 307–313
[17]      He R-F(何瑞锋), Ding Y(丁毅), Yu J-H(余金洪), Zu M-S(祖明生). Study on leaf ultrastructure of the thermo-sensitive chlorophyll deficient mutant in rice. J Wuhan Bot Res (武汉植物学研究), 2001, 19(1): 1–5 (in Chinese with English abstract)
[18]      Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832
[19]      Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325
[20]      Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas (Beijing) (遗传), 2003, 25(6): 705–707 (in Chinese with English abstract)
[21]      Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 259: 297–607
[22]      Liu M-M(刘梦梦), Sang X-C(桑贤春), Ling Y-H(凌英华), Du P(杜鹏), Zhao F-M(赵芳明), Yang Z-L(杨正林), He G-H(何光华). Genetic analysis and molecular mapping of a yellow-green leaf gene (YGL4)in rice (Oryza sativa L.). Acta Agron Sin (作物学报),2009, 35(8): 1405–1409 (in Chinese with English abstract)
[23]      Chen D-X (陈德西), Ma B-T (马炳田), Wang Y-P (王玉平), Li S-G (李仕贵), Hao M (郝铭). Morphological and anatomical characterization of a stripe mutant with abnormal floral organs in rice. J Mol Cell Biol (分子细胞生物学报), 2006, 39(4): 339–349 (in Chinese with English abstract)
[24]      Yu F, Fu A, Aluru M, Park S, Xu Y, Liu H, Liu X, Foudree A. Nambogga M, Rodermel S. Variegation mutants and mechanisms of chloroplast biogenesis. Plant Cell Environ, 2007, 30: 350–365
[25]      Aluru M R, Zola J, Foudree A, Rodermel S R. Chloroplast photooxidation-induced transcriptome reprogramming in Arabidopsis immutans white leaf sectors. Plant Physiol, 2009, 150: 904–923
[26]      Kato Y, Miura E, Matsushima R, Sakamoto W. White leaf sectors in yellow variegated2 are formed by viable cells with undifferentiated plastids. Plant Physiol, 2007, 144: 952–960
[27]      López-Juez E. Plastid biogenesis, between light and shadows. J Exp Bot, 2007, 58: 11–26
[28]      Gothandam K M, Kim E S, Cho H J, Chung Y Y. OsPPR1, a pentatricopeptide repeat protein of rice is essential for the chloroplast biogenesis. Plant Mol Biol, 2005, 58: 421–433
[29]      Tsugane K, Maekawa M, Takagi K, Takahara H, Qian Q, Eun C H, Iida S. An active DNA transposon nDart causing leaf variegation and mutable dwarfism and its related elements in rice. Plant J, 2006, 45: 46–57
[30]      Hu J-T(胡景涛), Zhang J(张甲), Li Y-Y(李园园), Fu C-Y(付崇允), Zheng J(郑静), Chen J-B(陈家彬), Hu Y(胡燕), Li S-G(李仕贵). Genetic analysis and mapping of a rice white midrib mutant Oswm2. Hereditas (Beijing) (遗传), 2008, 30(9): 1201–1206 (in Chinese with English abstract)
[31]      Yoo S C, Cho S H,Sugimoto H,Li J, Kusumi K, Koh H J, Iba K, Paek N C. Rice Virescent-3 and Stripe-1 encoding the large and small subunits of Ribonucleotide Reductase are required for chloroplast biogenesis during early leaf development. Plant Physiol, 2009, 150: 388–401
[1] YANG Si-Jie, DU Qi-Di, CHAI Shou-Xi, XIONG Hong-Chun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, GUO Hui-Jun, LIU Lu-Xiang. Genetic mapping of mutant genes on flag leaf length and width in wheat [J]. Acta Agronomica Sinica, 2025, 51(6): 1548-1557.
[2] YUAN Xin, ZHAO Zhuo-Fan, ZHAO Rui-Qing, LIU Xiao-Wei, ZHENG Ming-Min, LIU Yu-Sheng, DONG Hao-Sheng, DENG Li-Juan, CAO Mo-Ju, HUANG Qiang. Genetic analysis and molecular identification of a small kernel mutant mn-like1 in maize [J]. Acta Agronomica Sinica, 2025, 51(6): 1569-1581.
[3] WANG Rui, SUN Bo, ZHANG Yun-Long, ZHANG Ming-Qi, FAN Ya-Ming, TIAN Hong-Li, ZHAO Yi-Kun, YI Hong-Mei, KUANG Meng, WANG Feng-Ge. Application analysis of chloroplast markers on rapid classification in maize germplasm [J]. Acta Agronomica Sinica, 2024, 50(7): 1867-1876.
[4] SU Shuai, LIU Xiao-Wei, NIU Qun-Kai, SHI Zi-Wen, HOU Yu-Wei, FENG Kai-Jie, RONG Ting-Zhao, CAO Mo-Ju. Identification and gene cloning of leafy dwarf mutant lyd1 in maize [J]. Acta Agronomica Sinica, 2024, 50(5): 1124-1135.
[5] YU Yao, WANG Zi-Yao, ZHOU Si-Rui, LIU Peng-Cheng, YE Ya-Feng, MA Bo-Jun, LIU Bin-Mei, CHEN Xi-Feng. Phenotypic identification and disease resistance mechanism analysis of rice lesion mutant lms1 [J]. Acta Agronomica Sinica, 2024, 50(4): 857-870.
[6] JIA Lu-Qi, SUN You, TIAN Ran, ZHANG Xue-Fei, DAI Yong-Dong, CUI Zhi-Bo, LI Yang-Yang, FENG Xin-Yu, SANG Xian-Chun, and WANG Xiao-Wen. Identification of the rgs1 mutant with rapid germination of seed and isolation of the regulated gene in rice [J]. Acta Agronomica Sinica, 2023, 49(8): 2288-2295.
[7] TANG Jie, LONG Tuan, WU Chun-Yu, LI Xin-Peng, ZENG Xiang, WU Yong-Zhong, HUANG Pei-Jin. Identification of OsGMS2 and construction of seed production system for genic male sterile line in rice [J]. Acta Agronomica Sinica, 2023, 49(8): 2025-2038.
[8] WANG Xing-Rong, ZHANG Yan-Jun, TU Qi-Qi, GONG Dian-Ming, QIU Fa-Zhan. Identification and gene localization of a novel maize nuclear male sterility mutant ms6 [J]. Acta Agronomica Sinica, 2023, 49(8): 2077-2087.
[9] LIN Xiao-Xin, HUANG Ming-Jiang, WEI Yi, ZHU Hong-Hui, WANG Zi-Yi, LI Zhong-Cheng, ZHUANG Hui, LI Yan-Xi, LI Yun-Feng, CHEN Rui. Identification and gene mapping of long grain and degenerated palea (lgdp) in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2023, 49(6): 1699-1707.
[10] DAI Wen-Hui, ZHU Qi, ZHANG Xiao-Fang, LYU Shen-Yang, XIANG Xian-Bo, MA Tao, CHEN Yu-Jie, ZHU Shi-Hua, DING Wo-Na. Identification and gene mapping of brittle culm mutant bc21 in rice [J]. Acta Agronomica Sinica, 2023, 49(5): 1426-1431.
[11] CHEN Xiao-Han, WANG Li-Qin, WANG Hua-Dong, XIAO Qing, TAO Bao-Long, ZHAO Lun, WEN Jing, YI Bin, TU Jin-Xing, FU Ting-Dong, SHEN Jin-Xiong. BnABCI8 affects chloroplast development of Brassica napus [J]. Acta Agronomica Sinica, 2023, 49(4): 893-905.
[12] YAN Xin, XIANG Chao, LIU Rong, LI Guan, LI Meng-Wei, LI Zheng-Li, ZONG Xu-Xiao, YANG Tao. Fine mapping of flower colour gene in pea (Pisum sativum L.) based on BSA-seq technique [J]. Acta Agronomica Sinica, 2023, 49(4): 1006-1015.
[13] LI Qiu-Ping, ZHANG Chun-Long, YANG Hong, WANG Tuo, LI Juan, JIN Shou-Lin, HUANG Da-Jun, LI Dan-Dan, WEN Jian-Cheng. Physiological characteristics analysis and gene mapping of a semi-sterility plant mutant sfp10 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2023, 49(3): 634-646.
[14] CAO Xiao-Xiong, LIU Yi-Fan, ZHOU Yu-Qiang, WANG Jing, WU Yu-Jin, WANG Hong-Wu, LI Kun, LIU Xiao-Gang, HUANG Chang-Ling, LIU Zhi-Fang, GUO Jin-Jie, HU Xiao-Jiao. Genetic analysis and molecular identification of a multiple allele mutant of ZmMs7 gene in maize [J]. Acta Agronomica Sinica, 2023, 49(11): 2913-2922.
[15] DING Meng-Li, WANG Ru-Yin, SHI Dong-Sheng, LI Ying-Bo, LEI Jie, CHEN Hong-Yu, SHEN Qing-Wen, WANG Gui-Feng. Map-based cloning and transcriptomic analysis of a maize miniature kernel mutant mn-Mu [J]. Acta Agronomica Sinica, 2023, 49(11): 3122-3130.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd