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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (2): 211-216.doi: 10.3724/SP.J.1006.2010.00211


Identification and Molecular Mapping of Stripe Leaf Mutant st(t) in Rice (Oryza sativa L.)

SANG Xian-Chun,XU Fang-Fang,LING Ying-Hua,ZHAO Fang-Ming,YANG Zheng-Lin,TANG Yan-Qiang,TIAN Xiao-Qing,LI Yun-Feng,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Key Laboratory of Southwest Crop Genetic Improvement and Breeding, Ministry of Agriculture, Chongqing 400716, China
  • Received:2009-07-09 Revised:2009-10-08 Online:2010-02-10 Published:2009-12-21
  • Contact: HE Guang-hua,E-mail: hegh@swu.edu.cn E-mail:sangxianchun@163.com


A stripe leaf mutant temporarily designated as st(t) was discovered in the progeny of an excellent indica restorer line Jinhui10 seed by treatment of EMS (ethyl methane sulfonate) in Rice Research Institute of Southwest University. The st(t) mutant displayed the variegated leaves at three-leaf stage, the variegation developed the white irregular line stripe from elongating stage to the maturation. Compared with the wild type, the mutant decreased the chlorophyll content and increased the carotenoid content significantly. Cells structure was detected by Transmission Electron Microscopic (TEM), and the results indicated that there was no difference between the wild type and the green parts of the st(t), both of them could develop normal chloroplast; in the white parts of the st(t), cells developed abnormally contained irregular stromal lamellae, aberrant thylakoids and more osmiophilic granules. Genetic analysis suggested that the mutational characters were controlled by one nuclear recessive gene. Xinong1A was crossed with the st(t) and 1 500 mutants from the F2 population were used for gene mapping. Finally, St(t) gene was mapped between SSR marker RM19745 and RM19762 on the chromosome 6, the genetic distances were 0.07 cM and 0.27 cM respectively and the physical distance was 345 kb according to indica rice 9311. This result provides a foundation of map-based cloning of St(t) gene as well as its molecular marker-assisted breeding.

Key words: Stripe leaf, Rice(Oryza sativa L.), Chloroplast, Gene mapping

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