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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (3): 391-400.doi: 10.3724/SP.J.1006.2010.00391

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity and Relationship of Tea Germplasm in Yunnan Revealed by ISSR Analysis

LIU Ben-Ying1,2,LI You-Yong2,TANG Yi-Chun2,WANG Li-Yuan1,CHENG Hao1,*,WANG Ping-Sheng2   

  1. 1 Research Center for Tea Germplasm and Improvement, Tea Research Institute of Chinese Academy of Agricultural Science, National Center for Tea Improvement, Hangzhou 310008, China; 2 Tea Research Institute of Yunnan Academy of Agricultural Science, Menghai 666201, China
  • Received:2009-09-08 Revised:2009-12-08 Online:2010-03-12 Published:2010-01-22
  • Contact: CHENG Hao, E-mail: chenghao@mail.tricaas.com E-mail:liusuntao@126.com

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Abstract:

Tea is an important beverage crop in the world. Yunnan region in China is the origin center of tea plants. Our knowledge on genetic diversity and relationship of tea germplasm in Yunnan province is critical to guide tea breeding. In order to provide theoretical information for using tea germplasm in tea breeding, we investigated the relationship and genetic diversity of the tea germplasm in Yunnan province. Eight species, including 134 tea varieties were used to detect the genetic variation by inter-simple sequence repeats (ISSR) analysis. The result showed that 475 DNA fragments among all 134 tea accessions were amplified, using 18 reliable ISSR primers, among which 470 DNA bands were polymorphic (PPB=98.9%). This indicated that a great amount of genetic polymorphism exists among tea germplasm tested. The genetic similarity (GS) among the tested genotypes ranged from 0.445 to 0.819, with an average of 0.512, indicating a wide gene pool among tea varieties in Yunnan. The cluster analysis presented that these resources were divided into three main groups using the unweighted pair-group method with arithmetic average (UPGMA) based on ISSR molecular marker data, but the dendrogram did not indicate clear division among tested varieties based on their geographical origin. Principal Component analysis (PCA) for ISSR data showed that PCA supported UPGMA clustering result, but showed more explicit relationships among the test accessions with different lays, orientationsand positions. The GS among 8 populations ranged from 0.850 to 0.987, with an average of 0.92, indicating that there existed a small variation of genetic diversity among different population. The findings of this research would be favorable for the further practice, such as tea breeding, the molecular genetic linkage mapping and the DNA fingerprint building of tea germplasm.

Key words: Yunnan, Tea Germplasm, ISSR, Genetic diversity, Genetic Relationship


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