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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (06): 1050-1054.doi: 10.3724/SP.J.1006.2010.01050

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Genetic Analysis and Gene Mapping of a Novel Yellow-Green Leaf Mutant in Rice

LI Xiu-Lan1,2, SUN Xiao-Qiu2, WANG Ping-Rong2, ZHOU Hui2,DENG Xiao-Jian2,3,*
  

  1. 1College of Life Science,Qufu Normal University,Qufu 273165,china;2Rice Research Institute,Sichuan Agricultural University,Chengdu 611130,China;3Key Laboratory of Southwest Crop Genetic Resources and Improvement,Ministry of Education,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2010-01-06 Revised:2010-03-01 Online:2010-06-12 Published:2010-04-14
  • Contact: DENG Xiao-Jian, E-mail: xjdeng2006@yahoo.com.cn
  • About author:LI Xiu-Lan, E-mail: lxljewel@163.com

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Abstract:

An inherited stably yellow-green leaf mutant D83 was isolated by chemistry mutagenesis. Its whole plant exhibited yellow-green character at the seedling stage, and gradually changed into pea green from the tillering stage. Compared with its wild-type parent 10079, at the seedling stage, the contents of chlorophyll a, chlorophyll b and carotenoid decreased by 45.03%, 53.93% and 39.56%, at the maturation period, the number of spikelets per panicle reduced by 9.45%, the 1000-grain weight by decreased 10.76%, respectively. Genetic analysis of F1 and F2 generations of D83 crossed with green rice varieties showed that the yellow-green leaf character was controlled by one pair of recessive nuclear genes. Genetic mapping of the mutant gene was conducted by using molecular marker and F2 mapping population of D83/Zhefu802. The results suggested that the mutant gene of D83 was mapped on the short arm of rice chromosome 2, nearby SSR marker RM110, between InDel markers Ch2-27 and Ch2-32. The genetic distances from the target gene to two InDel markers were 1.2 cM and 2.3 cM, respectively. The mutant gene of D83 was considered to be a new yellow-green leaf gene in rice and designated tentatively as chl13(t).

Key words: Oryza sativa L., Yellow-green leaf mutant, Genetic analysis, Gene mapping

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