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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (03): 484-488.doi: 10.3724/SP.J.1006.2011.00484


Transgenic Tobacco Plants Resistant to Two Viruses via RNA Silencing

NIU Yan-Bing,WANG De-Fu,YAO Min,YAN Zhao,YOU Wen-Xin   

  1. College of Life Science, Shanxi Agricultural University, Taigu 030801, China
  • Received:2010-07-30 Revised:2010-11-26 Online:2011-03-12 Published:2011-01-17

Abstract: RNA silencing is a phenomenon of homologous RNA degradation induced by dsRNA, which is an effective strategy to obtain virus resistant plants so far. By means of this strategy, higher bio-security immune transgenic plants were obtained, avoiding recombining and transcapsidating with other virus genes. In this study, the recombinant plant expression vector pBIN438-MP- Rep(i/r)consisting the inverted repeat of TMV-ΔMP and CMV-ΔRep fusion fragment was transformed into tobacco cultivar K326 via Agrobacterium-mediated. The transformants were selected in the culture medium with 100 mg L–1 Kan. One hundred and ninety-six transgenic plants were obtained, One hundred and twenty-eight of which were positive plants, and the resistance to CMV and TMV was tested at the degree of virus. PCR-Southern blot and RT-PCR analysis of the transgenic plants demonstrated that the exogenous DNA was integrated into the tobacco genomic DNA and was expressed in transcriptional level. Resistance assay indicated that about 20.3% transgenic plants were immune to the co- infection with TMV and CMV. This result will provide to crops an important reference for plant anti-viral breeding and for preventing the viral co-infection.

Key words: RNA silencing, Cucumber mosaic virus, Tobacco mosaic virus, Dual-virus resistance

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