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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (03): 477-483.doi: 10.3724/SP.J.1006.2011.00477

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Overlap of Drought-Tolerance Loci between Germination Stage and Seedling Stage Analyzed Using Introgression Lines in Soybean

QIU Peng-Cheng1,2,ZHANG Wen-Bo1,2,LI Can-Dong4,JIANG Hong-Wei2,LIU Chun-Yan2,FAN Dong-Mei1,ZENG Qing-Li1,HU Guo-Hua1,3,*,CHEN Qing-Shan1,*   

  1. 1 College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 2 Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin 150090, China; 3 The National Research Center of Soybean Engineering and Technology, Harbin 150050, China; 4 Heilongjiang Academy of Agricultural Sciences Jiamusi Branch, Jiamusi 150030, China
  • Received:2010-07-29 Revised:2010-10-09 Online:2011-03-12 Published:2010-12-12
  • Contact: 胡国华, E-mail: Hugh757@vip.com, Tel:0451-55199475; 陈庆山, E-mail: qshchen@126.com, Tel: 0451-55191945

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Abstract: Soybean grows worldwide in many regions under drought stress, so drought tolerance (DT) is a very important trait for the crop. Most researches have focused on the QTL mapping related with drought-tolerance, but there is no research on the genetic overlap in soybean. A primary backcross introgression lines (ILs) were constructed with Hongfeng 11 as recurrent parent and Clark as donor parent. Forty-four individuals from BC1F5 introgression populations were screened out under drought stress comparing with the Hongfeng11 in germination stage, and 46 individuals were obtained under drought stress comparing with the control population in seedling stage. The QTL identification at germination stage and seedling stage was conducted by one-way ANOVA (for single marker analysis, P<0.05) with the two introgression populations. Then 10 QTLs were mapped in germination stage, 21 QTLs were mapped in seedling stage laced on relative water content(RWC)，water holding capacity(WHC), and growth of plant height(GPH). Four QTLs including Satt449, Satt499, Satt440, and Sat_180 in A1, K, I, and H linkage groups were mapped both in germination stage and seedling stage, indicating a partial genetic overlap between these two stages in soybean. The above results would provide a basic for fine mapping and molecular breeding for favorable genes related to DT in soybean.

Key words: Soybean, Introgression lines, Drought tolerance, Genetic overlap

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