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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 603-611.doi: 10.3724/SP.J.1006.2011.00603

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Identification of Drought Tolerance to Soybean in Selection Population

LI Can-Dong1,2,3,JIANG Hong-Wei1,LIU Chun-Yan1,GUO Tai2,WANG Zhi-Xin2,WU Xiu-Hong2,ZHENG Wei2,QIU Peng-Cheng3,ZHANG Wen-Bo3,SONG Ying-Bo2,LUAN Yi-Na5,CHEN Qing-Shan3,*,HU Guo-Hua1,4,*   

  1. 1 Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin 150090, China; 2 Jiamusi branch institute, Heilongjiang Academy of Agricultural Sciences, Jiamusi 154007, China; 3 College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 4The National Research Center of Soybean Engineering and Technology, Harbin 150050, China; 5 Heilongjiang Agricultural Vocational and Technical College, Jiamusi 154007, China
  • Received:2010-09-20 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 陈庆山, E-mail: qshchen@126.com, 0451-55191945; 胡国华, E-mail: Hugh757@vip.163.com, 0451-55199475

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Abstract: A primary backcross introgression of soybean population was constructed by using Hongfeng 11 as recurrent parent and Clark as donor parent. After screening under drought stress, the genotypes of selective population were obtained with the whole genome SSR markers, and the frequency of donor genes segments were analyzed. QTLs of five drought-tolerance traits were mapped by Chi-testcombined GGT linkage group analysis. In total, 23 SSR excessive introgression loci on 10 chromosomes were detected with χ2test. The QTL identification was conducted by one-way ANOVA (for single marker analysis, P<0.01). Eight QTLs of RWC (relative water content) were located on A1, B1, C2, E, L, and N linkage groups, nine QTLs of RRL (relative root length) on C2, F, G, and I linkage groups, 11 QTLs of RRW (relative root dry weight) on A2, B1, B2, E, F, K, L, M, and O linkage groups, 12 QTLs of RGY (relative grain yield) on B1, D1a, E, F, G, I, L, M, and O linkage groups and seven QTLs of RMB (relative microbial biomass) on E, F, G, K, L, and N linkage groups. The QTL at Sat_136 on E linkage group was identical for RWC, RRW, RGY, and RMB, and QTL at GMRUBP on F linkage group for RRW and RMB, QTL at Satt586 on F linkage group for RRL, RRW, and RGY, QTL at Satt167 on K linkage group for RRW and RMB, QTL at SOYPRP1 on K linkage group for RRL and RMB, QTL at Satt398 on L linkage group for RRL and RGY, QTL at Satt694 on L linkage group for RWC and RMB, QTL at GMSL514 on L linkage group for RRW and RGY. All above QTLs were coincident with those detected by excessive introgression of χ2 test. Thirty-three QTLs were mapped by χ2 test or one-way ANOVA, and among them 17 QTLs were detected by both methods. So these QTLs should be essential for drought tolerance. The results provide a foundation for fine mapping, cloning and molecular breeding of favorable genes related with drought tolerance.

Key words: Soybean, Drought tolerance, QTL identification

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