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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 784-792.doi: 10.3724/SP.J.1006.2011.00784

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of QTLs for Grain Traits in Rice Using Extreme Materials in Grain Size

ZHANG Qiang1,2,YAO Guo-Xin1,3,HU Guang-Long1,TANG Bo1,CHEN Chao1,LI Zi-Chao1,*   

  1. 1 Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture / Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; 2 Rice Research Institute, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China; 3 School of Life Science and Technology, Xiaogan University, Xiaogan 432100, China
  • Received:2010-10-19 Revised:2011-01-06 Online:2011-05-12 Published:2011-03-24

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Abstract: The grain length (GL), grain width (GW), grain thickness (GT), ratio of grain length to width (RLW), and 1000-grain weight (TGW) were evaluated in Beijing. Using a BC2F2 population including 216 lines derived from a backcross combination between GSL156 with large grain (71.9 g) and Chuanqi with small grain (12.1 g). The quantitative trait loci (QTLs) for above five grain traits were identified by composite interval mapping using SSR markers. The results showed that the five grain traits exhibited a normal continuous distribution in BC2F2 population, indicating that they were quantitative traits controlled by multiple genes. A total of 28 QTLs conferring the five grain traits were detected on chromosomes 1, 2, 3, 4, 5, 6, and 12, respectively. Fourteen QTLs, namely qGL3-2, qGL3-3, qGT12-1, qGT2-1, qGT5-1, qGW1-1, qGW12-1, qGW2-1, qGW5-1, qRLW3-1, qTGW12-1, qTGW2-1, qTGW3-3,and qTGW5-1, were main-effect QTLs and explained 13.70%, 52.51%, 21.13%, 18.79%, 20.92%, 14.59%, 18.33%, 30.03%, 20.05%, 24.53%, 13.47%, 11.43%, 21.30%, and 15.68% of the observed phenotypic variance, respectively. Among them, most QTLs were mapped on chromosome 3. Six QTLs had the alleles contributing to positive effect which were derived from small grain parent Chuanqi while the other 22 QTLs alleles from large grain parent GSL156. The modes of gene action were mainly additive or partial dominance. The marker interval RM7580–RM8208 on chromosome 3 was common to the three QTLs for GW, RLW, and TGW, respectively. The marker interval, RM7636–RM5812 on chromosome 2, RM3351–RM26 on chromosome 5, and RM1103–RM17 on chromosome 12, were common to the three major QTLs, which were associated with GW, GT and TGW, respectively.The eight SSR markers used in this study would be useful in molecular breeding in rice. The alleles from parent with larger grain were showed significant effects on GL, GW, GT, and TGW.

Key words: Rice, Grain trait, SSR marker, Quantitative trait locus

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