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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (07): 1205-1211.doi: 10.3724/SP.J.1006.2011.01205

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Activity Analysis of Soybean SACPD-C Promote

ZHANG Qing-Lin1,ZHAO Yan2,**,LI Xiao-Wei1,ZHAI Ying1,ZHANG Yan1,WANG Ying1,LI Jing-Wen1,*,WANG Qing-Yu1,*   

  1. 1 College of Plant Science, Jilin University, Changchun 130062, China; 2 College of Life Science and Agro-Forestry, Qiqihaer University, Qiqihaer 161006, China
  • Received:2011-01-04 Revised:2011-03-27 Online:2011-07-12 Published:2011-05-11
  • Contact: 王庆钰, E-mail: wqy414cn@yahoo.com.cn; 李景文, E-mail: lljjww998@sohu.com

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Abstract: The SACPD-Cp promoter of soybean SACPD-C was isolated from the genomic DNA of soybean Jidou 2 by TAIL PCR. Promoter sequence analysis by PLACE showed that the cloned fragment contained a lot of the motifs that constituted the seed-specific cis-elements. Replacing CaMV35S promoter of pCAMBIA1301 with the SACPD-Cp fragment, the binary expression vector pCAM-SACPD-Cp was constructed. Transient expression by Agrobacterium tumefaciens mediated method, the histochemical GUS analysis and fluorometric GUS analysis were used for testing the expression of the GUS activity. The results indicated that GUS activity driven by SACPD-Cp fragment was 93.01% of that driven by CaMV35S promoter. The SACPD-Cp promoter did not have the homology compared with the reported promoters. GUS activity assays indicated that GUS was expressed only in seeds, but not in roots, stems and leaves, which suggests the SACPD-Cp is seed-specific promoter.

Key words: Soybean, SACPD-C gene, Sequence analysis, Seed-specific promoter, Transient expression

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