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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (09): 1551-1558.doi: 10.3724/SP.J.1006.2011.01551

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Salt-tolerance Analysis of Gene Plastid Transcriptionally Active (GhPTAC) from Gossypium hirsutum L.

ZHOU Kai,YE Wu-Wei*,WANG Jun-Juan,WANG De-Long,FAN Bao-Xiang,WANG Shuai   

  1. Cotton Research Institute, Chinese Academy of Agricultural Science / State Key Laboratory of Cotton Biology / Key Laboratory of Cotton Genetic Improvement of Agriculture Ministry, Anyang 455000, China
  • Received:2011-02-28 Revised:2011-05-20 Online:2011-09-12 Published:2011-06-28
  • Contact: 叶武威, E-mail: yeww@cricaas.com.cn

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Abstract: To develop novel salt-tolerance genes and adjustment pathway, we screened out gene Plastid Transcriptionally Active named as GhPTAC based on salt-tolerance gene chips and salt resistance related SSH library.The result of RACE and RT-PCR showed that the cDNA full length was 1 564 bp, and ORF was 1 038 bp, which encoded 345 amino acid residues. Bioinformatics analysis showed that GhPTAC shared the identity of 60.6% with the homologous gene PTAC13 from Arabidopsis thaliana. As one part of Transcriptionally active chromosome (TAC), GhPTAC plays a part role in regulation of transcription termination/ antitermination of chloroplastid genome. The GhPTAC expression was up-regulated under salt stress induction and the expression level of GhPTAC of Zhong 9806 (salt-resistant material) was obviously higher than that Zhong S9612 (salt-sensitive material) which was measured by Real-time PCR and in accord with the arrays results.

Key words: Gossypium hirsutum, Salt stress, PTAC, Real-time PCR

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