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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 1009-1017.doi: 10.3724/SP.J.1006.2012.01009

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Characterization of Interaction between Wheat Roots with Different Resistance and Heterodera filipjevi

CUI Lei1,2,GAO Xiu2,4,WANG Xiao-Ming2,JIAN Heng3,TANG Wen-Hua3,LI Hong-Lian1,*,LI Hong-Jie2,*   

  1. 1 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China; 3 Department of Plant Pathology, China Agricultural University, Beijing 100193, China; 4 College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China
  • Received:2011-12-13 Revised:2012-02-22 Online:2012-06-12 Published:2012-03-29
  • Contact: 李洪杰, E-mail: hongjie@caas.net.cn; 李洪连, E-mail: honglianli@sina.com E-mail:cuileicomeon@yahoo.com.cn

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Abstract: Heterodera filipjevi is a newly identified pathogenic species of cereal cyst nematode that invades wheat (Triticum aestivum L.) in central China. A two-year field test (2009–2011) conducted in Xuchang County, Henan Province, China, where H. filipjevi has severely damaged wheat for several years, demonstrated that the wheat-rye (Secale cereale L.) chromosome substitution line 6R(6D) (carrying gene CreR for resistance to Heterodera spp.) was highly resistant (HR) to the nematode. The wheat cultivar Taikong 6 was moderately resistant (MR) and its parental cultivar Yumai 49 was highly susceptible (HS). Attraction of H. filipjevi juveniles to root tips of wheats differed in resistance to H. filipjevi was studied using Pluronic F-127 gel as a medium. Although root tips from wheat cultivars with different resistance were able to attract juveniles of nematode alone, the number of juveniles around the root tip from line 6R(6D) was obviously less than that from Taikong 6 (MR) and Yumai 49 (HS) when they were challenged together concurrently by H. filipjevi. The juveniles were capable to penetrate into wheat roots regardless of their resistance as revealed by acid fuschin-sodium hypochlorite staining of infected roots. However, significantly smaller number of nematodes in the roots of 6R(6D) and Taikong 6 were observed compared to those of Yumai 49 at later stage of wheat-H. filipjevi interaction. These findings demonstrate that some of the H. filipjevi juveniles are not able to continue to reproduce themselves although they can penetrate the roots of resistant wheat cultivars 6R(6D) and Taikong 6. The results from this study provide experimental evidence on mechanism of host resistance to H. filipjevi.

Key words: wheat, Heterodera filipjevi, resistance, infestation, attraction, wheat-nematode interaction

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