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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 1115-1120.doi: 10.3724/SP.J.1006.2012.01115

• RESEARCH NOTES • Previous Articles     Next Articles

Effect of Stresses on Leaf Cuticular Wax Accumulation and Its Relationship to Expression of OsGL1-Homologous Genes in Rice

ZHOU Ling-Yan1,2,JIANG Da-Gang2,LI Jing2,ZHOU Hai2,CAO Wei-Wei2,ZHUANG Chu-Xiong2,*   

  1. 1 College of Life Science, Zhongkai Agriculture and Engineering University, Guangzhou 510225, China; 2 College of Life Science, South China Agriculture University, Guangzhou 510642, China?
  • Received:2011-12-06 Revised:2012-02-22 Online:2012-06-12 Published:2012-04-06
  • Contact: 庄楚雄, E-mail: zhuangcx@scau.edu.cn, Tel: 020-85288399 E-mail:lingyanzh@163.com

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Abstract: The outermost surfaces of land plants are covered with a cuticular wax layer which plays a very critical role in defense against biotic and abiotic stresses. GLOSSY1 (GL1) is a wax biosynthesis related gene and OsGL1 is a homologous gene of GL1. In this study, we discussed the leaf cuticular wax accumulation and the relationship between the leaf cuticular wax accumulation and the expression of OsGL1 in rice treated with stresses, including 200 mmol L-1 NaCl, 12% PEG, 1.0% H2O2, high temperature and low temperature in their seedling stages. The scanning electron microscopy and the cuticular wax content analysis showed that the cuticular wax accumulation on leaves significantly increased under 12% PEG, 1.0% H2O2 and low temperature treatments. However, the cuticular wax accumulation on leaves declined slightly under NaCl and high temperature treatments. Results of RT-PCR analysis suggested that the expression of OsGL1 under stresses was associated with the accumulation of cuticular wax on leaves.

Key words: Oryza sativa L., Stress treatments, Cuticular wax, OsGL1, Expression

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