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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (12): 2306-2311.doi: 10.3724/SP.J.1006.2012.02306

• RESEARCH NOTES • Previous Articles     Next Articles

Cloning and Characterization of ACC Synthase Gene (BnACS1) from Ramie (Boehmeria nivea)

ZHOU Jing-Hua1, YU Wei-Lin1, XING Hu-Cheng1,2,*, JIE Yu-Cheng1,2, ZHONG Ying-Li1,3,JING Li-Heng1   

  1. 1 Institute of Ramie, Hunan Agricultural University, Changsha 410128, China; 2 Hunan Provincial Key Laboratory of Corp Germplasm Innovation and Utilization, Changsha 410128, China; 3 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
  • Received:2012-04-27 Revised:2012-08-15 Online:2012-12-12 Published:2012-10-08
  • Contact: 邢虎成, E-mail: xhcsoldier@163.com, Tel: 15274806570

Abstract:

According to the ACS gene sequence from ramie transcriptome, we designed primers and cloned a full-length sequence of ACS gene cDNA from Xiangzhu 3 by RT-PCR and RACE methods, named as BnACS1, with the accession number of JQ970520 in GenBank. The full length sequence and the ORF of the BnACS1 gene were 1 674 bp and 1 470 bp, respectively, which encoded 489 amino acids. The molecule weight was 54.55 kD and the pI was 6.37. The similarity comparison revealed that the gene nucleotide sequence shared 74%, 74%, 72%, 71%, 70%, and 70% of homology with the Malus × domestica (AB034993), Eriobotrya japonica (GQ370520), Momordica charantia (AF248734), Paeonia suffruticosa (DQ337250), Nicotiana attenuate (AY426755), and Populus euphratica (AB033502) ACS gene, and the similarity of the amino acid sequences with that of those species was 75%, 74%, 71%, 71%, 70%, and 74%, respectively. The results of semi-quantitative RT-PCR showed that the BnACS1 expressed in root, stem, shoot tip, blade, female flower andmale flower, with the higher expression level in root and male flower, while the lowest expression level in stem. The results of real-time PCR showed that the BnACS1 was induced by dehydration and ABA, but not by high salt.

Key words: Ramie, Ethylene, ACC Synthase, ACS, Cloning and expression

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